CRISPR/Cas9-mediated viral interference in plants

Handle URI:
http://hdl.handle.net/10754/582543
Title:
CRISPR/Cas9-mediated viral interference in plants
Authors:
Ali, Zahir ( 0000-0002-7814-8908 ) ; Abulfaraj, Aala Abdulaziz Hussien; Idris, Ali; Ali, Shawkat ( 0000-0003-3282-3186 ) ; Tashkandi, Manal; Mahfouz, Magdy M. ( 0000-0002-0616-6365 )
Abstract:
Background The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.
KAUST Department:
Laboratory for Genome Engineering; Center for Desert Agriculture; Biological Sciences
Citation:
CRISPR/Cas9-mediated viral interference in plants 2015, 16 (1) Genome Biology
Publisher:
Springer Nature
Journal:
Genome Biology
Issue Date:
11-Nov-2015
DOI:
10.1186/s13059-015-0799-6
Type:
Article
ISSN:
1474-760X
Is Supplemented By:
Ali, Z., Aala Abulfaraj, Idris, A., Ali, S., Tashkandi, M., & Magdy Mahfouz. (2015). CRISPR/Cas9-mediated viral interference in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3612878; DOI:10.6084/m9.figshare.c.3612878; HANDLE:http://hdl.handle.net/10754/624131
Additional Links:
http://genomebiology.com/2015/16/1/238
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorAli, Zahiren
dc.contributor.authorAbulfaraj, Aala Abdulaziz Hussienen
dc.contributor.authorIdris, Alien
dc.contributor.authorAli, Shawkaten
dc.contributor.authorTashkandi, Manalen
dc.contributor.authorMahfouz, Magdy M.en
dc.date.accessioned2015-11-23T13:55:28Zen
dc.date.available2015-11-23T13:55:28Zen
dc.date.issued2015-11-11en
dc.identifier.citationCRISPR/Cas9-mediated viral interference in plants 2015, 16 (1) Genome Biologyen
dc.identifier.issn1474-760Xen
dc.identifier.doi10.1186/s13059-015-0799-6en
dc.identifier.urihttp://hdl.handle.net/10754/582543en
dc.description.abstractBackground The CRISPR/Cas9 system provides bacteria and archaea with molecular immunity against invading phages and conjugative plasmids. Recently, CRISPR/Cas9 has been used for targeted genome editing in diverse eukaryotic species. Results In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably overexpressing the Cas9 endonuclease, and subsequently challenge these plants with TYLCV. Our data demonstrate that the CRISPR/Cas9 system targeted TYLCV for degradation and introduced mutations at the target sequences. All tested sgRNAs exhibit interference activity, but those targeting the stem-loop sequence within the TYLCV origin of replication in the intergenic region (IR) are the most effective. N. benthamiana plants expressing CRISPR/Cas9 exhibit delayed or reduced accumulation of viral DNA, abolishing or significantly attenuating symptoms of infection. Moreover, this system could simultaneously target multiple DNA viruses. Conclusions These data establish the efficacy of the CRISPR/Cas9 system for viral interference in plants, thereby extending the utility of this technology and opening the possibility of producing plants resistant to multiple viral infections.en
dc.language.isoenen
dc.publisherSpringer Natureen
dc.relation.urlhttp://genomebiology.com/2015/16/1/238en
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.subjectPlant genome engineeringen
dc.subjectCRISPR/Cas9 systemen
dc.subjectSynthetic site-specific nucleasesen
dc.subjectViral-mediated genome editingen
dc.subjectVirus resistanceen
dc.subjectTomato yellow leaf curl virusen
dc.subjectTobacco rattle virusen
dc.titleCRISPR/Cas9-mediated viral interference in plantsen
dc.typeArticleen
dc.contributor.departmentLaboratory for Genome Engineeringen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological Sciencesen
dc.identifier.journalGenome Biologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAli, Zahiren
kaust.authorAbulfaraj, Aala Abdulaziz Hussienen
kaust.authorIdris, Alien
kaust.authorAli, Shawkaten
kaust.authorTashkandi, Manalen
kaust.authorMahfouz, Magdy M.en
dc.relation.isSupplementedByAli, Z., Aala Abulfaraj, Idris, A., Ali, S., Tashkandi, M., & Magdy Mahfouz. (2015). CRISPR/Cas9-mediated viral interference in plants. Figshare. https://doi.org/10.6084/m9.figshare.c.3612878en
dc.relation.isSupplementedByDOI:10.6084/m9.figshare.c.3612878en
dc.relation.isSupplementedByHANDLE:http://hdl.handle.net/10754/624131en
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