Sample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans

Handle URI:
http://hdl.handle.net/10754/582473
Title:
Sample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans
Authors:
Teira, Eva; Hernando-Morales, Víctor; Cornejo-Castillo, Francisco M.; Alonso-Sáez, Laura; Sarmento, Hugo; Valencia-Vila, Joaquín; Serrano Catalá, Teresa; Hernández-Ruiz, Marta; Varela, Marta M.; Ferrera, Isabel; Moran, Xose Anxelu G. ( 0000-0002-9823-5339 ) ; Gasol, Josep M.
Abstract:
The transformation of leucine incorporation rates to prokaryotic carbon production rates requires the use of either theoretical or empirically determined conversion factors. Empirical leucine-to-carbon conversion factors (eCFs) vary widely across environments, and little is known about their potential controlling factors. We conducted 10 surface seawater manipulation experiments across the world's oceans, where the growth of the natural prokaryotic assemblages was promoted by filtration (i.e., removal of grazers [F treatment]) or filtration combined with dilution (i.e., also relieving resource competition [FD treatment]). The impact of sunlight exposure was also evaluated in the FD treatments, and we did not find a significant effect on the eCFs. The eCFs varied from 0.09 to 1.47 kg C mol Leu−1 and were significantly lower in the FD than in the F samples. Also, changes in bacterial community composition during the incubations, as assessed by automated ribosomal intergenic spacer analysis (ARISA), were more pronounced in the FD than in the F treatments, compared to unmanipulated controls. Thus, we discourage the common procedure of diluting samples (in addition to filtration) for eCF determination. The eCFs in the filtered treatment were negatively correlated with the initial chlorophyll a concentration, picocyanobacterial abundance (mostly Prochlorococcus), and the percentage of heterotrophic prokaryotes with high nucleic acid content (%HNA). The latter two variables explained 80% of the eCF variability in the F treatment, supporting the view that both Prochlorococcus and HNA prokaryotes incorporate leucine in substantial amounts, although this results in relatively low carbon production rates in the oligotrophic ocean.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Sample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans 2015, 81 (23):8224 Applied and Environmental Microbiology
Publisher:
American Society for Microbiology
Journal:
Applied and Environmental Microbiology
Issue Date:
25-Sep-2015
DOI:
10.1128/AEM.02454-15
Type:
Article
ISSN:
0099-2240; 1098-5336
Additional Links:
http://aem.asm.org/lookup/doi/10.1128/AEM.02454-15
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorTeira, Evaen
dc.contributor.authorHernando-Morales, Víctoren
dc.contributor.authorCornejo-Castillo, Francisco M.en
dc.contributor.authorAlonso-Sáez, Lauraen
dc.contributor.authorSarmento, Hugoen
dc.contributor.authorValencia-Vila, Joaquínen
dc.contributor.authorSerrano Catalá, Teresaen
dc.contributor.authorHernández-Ruiz, Martaen
dc.contributor.authorVarela, Marta M.en
dc.contributor.authorFerrera, Isabelen
dc.contributor.authorMoran, Xose Anxelu G.en
dc.contributor.authorGasol, Josep M.en
dc.date.accessioned2015-11-22T12:09:02Zen
dc.date.available2015-11-22T12:09:02Zen
dc.date.issued2015-09-25en
dc.identifier.citationSample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceans 2015, 81 (23):8224 Applied and Environmental Microbiologyen
dc.identifier.issn0099-2240en
dc.identifier.issn1098-5336en
dc.identifier.doi10.1128/AEM.02454-15en
dc.identifier.urihttp://hdl.handle.net/10754/582473en
dc.description.abstractThe transformation of leucine incorporation rates to prokaryotic carbon production rates requires the use of either theoretical or empirically determined conversion factors. Empirical leucine-to-carbon conversion factors (eCFs) vary widely across environments, and little is known about their potential controlling factors. We conducted 10 surface seawater manipulation experiments across the world's oceans, where the growth of the natural prokaryotic assemblages was promoted by filtration (i.e., removal of grazers [F treatment]) or filtration combined with dilution (i.e., also relieving resource competition [FD treatment]). The impact of sunlight exposure was also evaluated in the FD treatments, and we did not find a significant effect on the eCFs. The eCFs varied from 0.09 to 1.47 kg C mol Leu−1 and were significantly lower in the FD than in the F samples. Also, changes in bacterial community composition during the incubations, as assessed by automated ribosomal intergenic spacer analysis (ARISA), were more pronounced in the FD than in the F treatments, compared to unmanipulated controls. Thus, we discourage the common procedure of diluting samples (in addition to filtration) for eCF determination. The eCFs in the filtered treatment were negatively correlated with the initial chlorophyll a concentration, picocyanobacterial abundance (mostly Prochlorococcus), and the percentage of heterotrophic prokaryotes with high nucleic acid content (%HNA). The latter two variables explained 80% of the eCF variability in the F treatment, supporting the view that both Prochlorococcus and HNA prokaryotes incorporate leucine in substantial amounts, although this results in relatively low carbon production rates in the oligotrophic ocean.en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.relation.urlhttp://aem.asm.org/lookup/doi/10.1128/AEM.02454-15en
dc.rightsArchived with thanks to Applied and Environmental Microbiologyen
dc.titleSample Dilution and Bacterial Community Composition Influence Empirical Leucine-to-Carbon Conversion Factors in Surface Waters of the World's Oceansen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalApplied and Environmental Microbiologyen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionUniversidade de Vigo, Vigo, Spainen
dc.contributor.institutionInstitut de Ciències del Mar, CSIC, Barcelona, Spainen
dc.contributor.institutionAZTI, Sukarrieta, Spainen
dc.contributor.institutionDepartment of Hydrobiology, Federal University of São Carlos, São Carlos, São Paulo, Brazilen
dc.contributor.institutionInstituto Español de Oceanografía, Centro Oceanográfico de A Coruña, A Coruña, Spainen
dc.contributor.institutionUniversidad de Granada, Granada, Spainen
dc.contributor.institutionInstituto Español de Oceanografía, Centro Oceanográfico de Xixón, Xixón, Spainen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
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