A System-Wide Approach to Identify the Mechanisms of Barnacle Attachment: Toward the Discovery of New Antifouling Compounds

Handle URI:
http://hdl.handle.net/10754/582250
Title:
A System-Wide Approach to Identify the Mechanisms of Barnacle Attachment: Toward the Discovery of New Antifouling Compounds
Authors:
Al-Aqeel, Sarah ( 0000-0002-4918-5849 )
Abstract:
Biofouling is a significant economic problem, particularly for marine and offshore oil industries. The acorn barnacle (Amphibalanus (Balanus) amphitrite) is the main biofouling organism in marine environments. Environmental conditions, the physiology of the biofouling organism, the surrounding microbial community, and the properties of the substratum can all influence the attachment of biofouling organisms to substrates. My dissertation investigated the biological processes involved in B. amphitrite development and attachment in the unique environment of the Red Sea, where the average water surface temperature is 34°C and the salinity reaches 41‰. I profiled the transcriptome and proteome of B. amphitrite at different life stages (nauplius II, nauplius VI, and cyprid) and identified 65,784 expressed contigs and 1387 expressed proteins by quantitative proteomics. During the planktonic stage, genes related to osmotic stress, salt stress, the hyperosmotic response, and the Wnt signaling pathway were strongly up-regulated, hereas genes related to the MAPK pathway, lipid metabolism, and cuticle development were down-regulated. In the transition from the nauplius VI to cyprid stages, there was up-regulation of genes involved in blood coagulation, cuticle development, and eggshell formation, and down-regulation of genes in the nitric oxide pathway, which stimulates the swimming and feeding responses of marine invertebrates. This system-wide integrated approach elucidated the development and attachment pathways important in B. amphitrite. Enzymes and metabolites in these pathways are potential molecular targets for the development of new antifouling compounds.
Advisors:
Ravasi, Timothy ( 0000-0002-9950-465X )
Committee Member:
Qian, Pei-Yuan ( 0000-0003-4074-9078 ) ; Pinnau, Ingo ( 0000-0003-3040-9088 ) ; Voolstra, Christian R. ( 0000-0003-4555-3795 )
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Chemical and Biological Engineering Program
Program:
Chemical and Biological Engineering
Issue Date:
Nov-2015
Type:
Dissertation
Appears in Collections:
Dissertations; Physical Sciences and Engineering (PSE) Division; Chemical and Biological Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.advisorRavasi, Timothyen
dc.contributor.authorAl-Aqeel, Sarahen
dc.date.accessioned2015-11-17T08:22:37Zen
dc.date.available2015-11-17T08:22:37Zen
dc.date.issued2015-11en
dc.identifier.urihttp://hdl.handle.net/10754/582250en
dc.description.abstractBiofouling is a significant economic problem, particularly for marine and offshore oil industries. The acorn barnacle (Amphibalanus (Balanus) amphitrite) is the main biofouling organism in marine environments. Environmental conditions, the physiology of the biofouling organism, the surrounding microbial community, and the properties of the substratum can all influence the attachment of biofouling organisms to substrates. My dissertation investigated the biological processes involved in B. amphitrite development and attachment in the unique environment of the Red Sea, where the average water surface temperature is 34°C and the salinity reaches 41‰. I profiled the transcriptome and proteome of B. amphitrite at different life stages (nauplius II, nauplius VI, and cyprid) and identified 65,784 expressed contigs and 1387 expressed proteins by quantitative proteomics. During the planktonic stage, genes related to osmotic stress, salt stress, the hyperosmotic response, and the Wnt signaling pathway were strongly up-regulated, hereas genes related to the MAPK pathway, lipid metabolism, and cuticle development were down-regulated. In the transition from the nauplius VI to cyprid stages, there was up-regulation of genes involved in blood coagulation, cuticle development, and eggshell formation, and down-regulation of genes in the nitric oxide pathway, which stimulates the swimming and feeding responses of marine invertebrates. This system-wide integrated approach elucidated the development and attachment pathways important in B. amphitrite. Enzymes and metabolites in these pathways are potential molecular targets for the development of new antifouling compounds.en
dc.language.isoenen
dc.subjectBarnacleen
dc.subjectBiofoulingen
dc.subjectTranscriptomeen
dc.subjectProteomeen
dc.subjectAttachmenten
dc.subjectAntifoulingen
dc.titleA System-Wide Approach to Identify the Mechanisms of Barnacle Attachment: Toward the Discovery of New Antifouling Compoundsen
dc.typeDissertationen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentChemical and Biological Engineering Programen
thesis.degree.grantorKing Abdullah University of Science and Technologyen_GB
dc.contributor.committeememberQian, Pei-Yuanen
dc.contributor.committeememberPinnau, Ingoen
dc.contributor.committeememberVoolstra, Christian R.en
thesis.degree.disciplineChemical and Biological Engineeringen
thesis.degree.nameDoctor of Philosophyen
dc.person.id102157en
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