Membrane biofouling in a wastewater nitrification reactor: microbial succession from autotrophic colonization to heterotrophic domination

Handle URI:
http://hdl.handle.net/10754/581499
Title:
Membrane biofouling in a wastewater nitrification reactor: microbial succession from autotrophic colonization to heterotrophic domination
Authors:
Lu, Huijie; Xue, Zheng; Saikaly, Pascal ( 0000-0001-7678-3986 ) ; Nunes, Suzana Pereira ( 0000-0002-3669-138X ) ; Bluver, Ted R.; Liu, Wen-Tso
Abstract:
Membrane biofouling is a complex process that involves bacterial adhesion, extracellular polymeric substances (EPS) excretion and utilization, and species interactions. To obtain a better understanding of the microbial ecology of biofouling process, this study conducted rigorous, time-course analyses on the structure, EPS and microbial composition of the fouling layer developed on ultrafiltration membranes in a nitrification bioreactor. During a 14-day fouling event, three phases were determined according to the flux decline and microbial succession patterns. In Phase I (0-2 days), small sludge flocs in the bulk liquid were selectively attached on membrane surfaces, leading to the formation of similar EPS and microbial community composition as the early biofilms. Dominant populations in small flocs, e.g., Nitrosomonas, Nitrobacter, and Acinetobacter spp., were also the major initial colonizers on membranes. In Phase II (2-4 d), fouling layer structure, EPS composition, and bacterial community went through significant changes. Initial colonizers were replaced by fast-growing and metabolically versatile heterotrophs (e.g., unclassified Sphingobacteria). The declining EPS polysaccharide to protein (PS:PN) ratios could be correlated well with the increase in microbial community diversity. In Phase III (5-14 d), heterotrophs comprised over 90% of the community, whereas biofilm structure and EPS composition remained relatively stable. In all phases, AOB and NOB were constantly found within the top 40% of the fouling layer, with the maximum concentrations around 15% from the top. The overall microbial succession pattern from autotrophic colonization to heterotrophic domination implied that MBR biofouling could be alleviated by forming larger bacterial flocs in bioreactor suspension (reducing autotrophic colonization), and by designing more specific cleaning procedures targeting dominant heterotrophs during typical filtration cycles.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Membrane biofouling in a wastewater nitrification reactor: microbial succession from autotrophic colonization to heterotrophic domination 2015 Water Research
Publisher:
Elsevier BV
Journal:
Water Research
Issue Date:
22-Oct-2015
DOI:
10.1016/j.watres.2015.10.013
Type:
Article
ISSN:
00431354
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0043135415302785
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLu, Huijieen
dc.contributor.authorXue, Zhengen
dc.contributor.authorSaikaly, Pascalen
dc.contributor.authorNunes, Suzana Pereiraen
dc.contributor.authorBluver, Ted R.en
dc.contributor.authorLiu, Wen-Tsoen
dc.date.accessioned2015-11-01T11:04:25Zen
dc.date.available2015-11-01T11:04:25Zen
dc.date.issued2015-10-22en
dc.identifier.citationMembrane biofouling in a wastewater nitrification reactor: microbial succession from autotrophic colonization to heterotrophic domination 2015 Water Researchen
dc.identifier.issn00431354en
dc.identifier.doi10.1016/j.watres.2015.10.013en
dc.identifier.urihttp://hdl.handle.net/10754/581499en
dc.description.abstractMembrane biofouling is a complex process that involves bacterial adhesion, extracellular polymeric substances (EPS) excretion and utilization, and species interactions. To obtain a better understanding of the microbial ecology of biofouling process, this study conducted rigorous, time-course analyses on the structure, EPS and microbial composition of the fouling layer developed on ultrafiltration membranes in a nitrification bioreactor. During a 14-day fouling event, three phases were determined according to the flux decline and microbial succession patterns. In Phase I (0-2 days), small sludge flocs in the bulk liquid were selectively attached on membrane surfaces, leading to the formation of similar EPS and microbial community composition as the early biofilms. Dominant populations in small flocs, e.g., Nitrosomonas, Nitrobacter, and Acinetobacter spp., were also the major initial colonizers on membranes. In Phase II (2-4 d), fouling layer structure, EPS composition, and bacterial community went through significant changes. Initial colonizers were replaced by fast-growing and metabolically versatile heterotrophs (e.g., unclassified Sphingobacteria). The declining EPS polysaccharide to protein (PS:PN) ratios could be correlated well with the increase in microbial community diversity. In Phase III (5-14 d), heterotrophs comprised over 90% of the community, whereas biofilm structure and EPS composition remained relatively stable. In all phases, AOB and NOB were constantly found within the top 40% of the fouling layer, with the maximum concentrations around 15% from the top. The overall microbial succession pattern from autotrophic colonization to heterotrophic domination implied that MBR biofouling could be alleviated by forming larger bacterial flocs in bioreactor suspension (reducing autotrophic colonization), and by designing more specific cleaning procedures targeting dominant heterotrophs during typical filtration cycles.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0043135415302785en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Water Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Water Research, 22 October 2015. DOI: 10.1016/j.watres.2015.10.013en
dc.subjectnitrificationen
dc.subjectbiofoulingen
dc.subjectbiofilm structureen
dc.subjectbacterial successionen
dc.subjectsubmerged MBRen
dc.titleMembrane biofouling in a wastewater nitrification reactor: microbial succession from autotrophic colonization to heterotrophic dominationen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalWater Researchen
dc.eprint.versionPost-printen
dc.contributor.institutionDepartment of Civil and Environmental Engineering, University of Illinois at Urbana Champaign, Urbana, IL, United Statesen
dc.contributor.institutionDepartment of Civil and Environmental Engineering, University of Vermont, Burlington, VT, United Statesen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorSaikaly, Pascalen
kaust.authorNunes, Suzana Pereiraen
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