A simple system for in-droplet incubation and quantification of agglutination assays

Handle URI:
http://hdl.handle.net/10754/580854
Title:
A simple system for in-droplet incubation and quantification of agglutination assays
Authors:
Castro, David; Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Foulds, Ian G.
Abstract:
This work reports on a simple system for quantitative sensing of a target analyte based on agglutination in micro-channels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (~2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.
KAUST Department:
Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Publisher:
Royal Society of Chemistry
Journal:
MicroTAS conference proceedings
Conference/Event name:
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
Issue Date:
28-Oct-2013
Type:
Conference Paper
ISBN:
978-0-9798064-6-9
Additional Links:
http://www.microtas2013.org/
Appears in Collections:
Conference Papers; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorCastro, Daviden
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorFoulds, Ian G.en
dc.date.accessioned2015-10-25T06:06:58Zen
dc.date.available2015-10-25T06:06:58Zen
dc.date.issued2013-10-28en
dc.identifier.isbn978-0-9798064-6-9en
dc.identifier.urihttp://hdl.handle.net/10754/580854en
dc.description.abstractThis work reports on a simple system for quantitative sensing of a target analyte based on agglutination in micro-channels. Functionalized microbeads and analyte with no prior incubation are flowed in droplets (~2μL) through a thin silicone tube filled with mineral oil at a flow rate of 150 μL/min. Hydrodynamic forces alone produce a highly efficient mixing of the beads within the droplet, without the need of complex mixing structures or magnetic actuation. The setup allows rapid observation of agglutination (<2 min), which is quantified using image analysis, and has potential application to high-throughput analysis.en
dc.publisherRoyal Society of Chemistryen
dc.relation.urlhttp://www.microtas2013.org/en
dc.subjectAgglutinationen
dc.subjectDNAen
dc.subjectProteinen
dc.subjectMicrobeadsen
dc.titleA simple system for in-droplet incubation and quantification of agglutination assaysen
dc.typeConference Paperen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalMicroTAS conference proceedingsen
dc.conference.date2013.10.27 - 2013.10.31en
dc.conference.name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)en
dc.conference.locationFreiburg, Germanyen
dc.eprint.versionPublisher's Version/PDFen
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