A SWI/SNF Chromatin Remodelling Protein Controls Cytokinin Production through the Regulation of Chromatin Architecture

Handle URI:
http://hdl.handle.net/10754/579847
Title:
A SWI/SNF Chromatin Remodelling Protein Controls Cytokinin Production through the Regulation of Chromatin Architecture
Authors:
Jégu, Teddy; Domenichini, Séverine; Blein, Thomas; Ariel, Federico; Christ, Aurélie; Kim, SoonKap; Crespi, Martin; Boutet-Mercey, Stéphanie; Mouille, Grégory; Bourge, Mickaël; Hirt, Heribert ( 0000-0003-3119-9633 ) ; Bergounioux, Catherine; Raynaud, Cécile; Benhamed, Moussa ( 0000-0002-4181-1702 )
Abstract:
Chromatin architecture determines transcriptional accessibility to DNA and consequently gene expression levels in response to developmental and environmental stimuli. Recently, chromatin remodelers such as SWI/SNF complexes have been recognized as key regulators of chromatin architecture. To gain insight into the function of these complexes during root development, we have analyzed Arabidopsis knock-down lines for one sub-unit of SWI/SNF complexes: BAF60. Here, we show that BAF60 is a positive regulator of root development and cell cycle progression in the root meristem via its ability to down-regulate cytokinin production. By opposing both the deposition of active histone marks and the formation of a chromatin regulatory loop, BAF60 negatively regulates two crucial target genes for cytokinin biosynthesis (IPT3 and IPT7) and one cell cycle inhibitor (KRP7). Our results demonstrate that SWI/SNF complexes containing BAF60 are key factors governing the equilibrium between formation and dissociation of a chromatin loop controlling phytohormone production and cell cycle progression.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
A SWI/SNF Chromatin Remodelling Protein Controls Cytokinin Production through the Regulation of Chromatin Architecture 2015, 10 (10):e0138276 PLOS ONE
Publisher:
Public Library of Science (PLoS)
Journal:
PLoS ONE
Issue Date:
12-Oct-2015
DOI:
10.1371/journal.pone.0138276
Type:
Article
ISSN:
1932-6203
Additional Links:
http://dx.plos.org/10.1371/journal.pone.0138276
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorJégu, Teddyen
dc.contributor.authorDomenichini, Séverineen
dc.contributor.authorBlein, Thomasen
dc.contributor.authorAriel, Federicoen
dc.contributor.authorChrist, Aurélieen
dc.contributor.authorKim, SoonKapen
dc.contributor.authorCrespi, Martinen
dc.contributor.authorBoutet-Mercey, Stéphanieen
dc.contributor.authorMouille, Grégoryen
dc.contributor.authorBourge, Mickaëlen
dc.contributor.authorHirt, Heriberten
dc.contributor.authorBergounioux, Catherineen
dc.contributor.authorRaynaud, Cécileen
dc.contributor.authorBenhamed, Moussaen
dc.date.accessioned2015-10-18T14:48:16Zen
dc.date.available2015-10-18T14:48:16Zen
dc.date.issued2015-10-12en
dc.identifier.citationA SWI/SNF Chromatin Remodelling Protein Controls Cytokinin Production through the Regulation of Chromatin Architecture 2015, 10 (10):e0138276 PLOS ONEen
dc.identifier.issn1932-6203en
dc.identifier.doi10.1371/journal.pone.0138276en
dc.identifier.urihttp://hdl.handle.net/10754/579847en
dc.description.abstractChromatin architecture determines transcriptional accessibility to DNA and consequently gene expression levels in response to developmental and environmental stimuli. Recently, chromatin remodelers such as SWI/SNF complexes have been recognized as key regulators of chromatin architecture. To gain insight into the function of these complexes during root development, we have analyzed Arabidopsis knock-down lines for one sub-unit of SWI/SNF complexes: BAF60. Here, we show that BAF60 is a positive regulator of root development and cell cycle progression in the root meristem via its ability to down-regulate cytokinin production. By opposing both the deposition of active histone marks and the formation of a chromatin regulatory loop, BAF60 negatively regulates two crucial target genes for cytokinin biosynthesis (IPT3 and IPT7) and one cell cycle inhibitor (KRP7). Our results demonstrate that SWI/SNF complexes containing BAF60 are key factors governing the equilibrium between formation and dissociation of a chromatin loop controlling phytohormone production and cell cycle progression.en
dc.language.isoenen
dc.publisherPublic Library of Science (PLoS)en
dc.relation.urlhttp://dx.plos.org/10.1371/journal.pone.0138276en
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/4.0/en
dc.titleA SWI/SNF Chromatin Remodelling Protein Controls Cytokinin Production through the Regulation of Chromatin Architectureen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalPLoS ONEen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionInstitut de Biologie des Plantes, UMR8618 CNRS-Université Paris-Sud XI, Saclay Plant Sciences, Orsay, Franceen
dc.contributor.institutionInstitut des Sciences du Végétal, UPR2355 CNRS, Saclay Plant Sciences, Gif-sur-Yvette, Franceen
dc.contributor.institutionInstitut Jean-Pierre Bourgin, UMR1318 INRA/AgroParisTech, Versailles, Franceen
dc.contributor.institutionPôle de Biologie Cellulaire, Imagif, Centre de Recherche de Gif, CNRS, IFR87, Gif-sur-Yvette, Franceen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorKim, SoonKapen
kaust.authorHirt, Heriberten
kaust.authorBenhamed, Moussaen
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