Contemporary molecular tools in microbial ecology and their application to advancing biotechnology

Handle URI:
http://hdl.handle.net/10754/578848
Title:
Contemporary molecular tools in microbial ecology and their application to advancing biotechnology
Authors:
Rashid, Mamoon ( 0000-0003-4851-4994 ) ; Stingl, Ulrich ( 0000-0002-0684-2597 )
Abstract:
Novel methods in microbial ecology are revolutionizing our understanding of the structure and function of microbes in the environment, but concomitant advances in applications of these tools to biotechnology are mostly lagging behind. After more than a century of efforts to improve microbial culturing techniques, about 70–80% of microbial diversity – recently called the “microbial dark matter” – remains uncultured. In early attempts to identify and sample these so far uncultured taxonomic lineages, methods that amplify and sequence ribosomal RNA genes were extensively used. Recent developments in cell separation techniques, DNA amplification, and high-throughput DNA sequencing platforms have now made the discovery of genes/genomes of uncultured microorganisms from different environments possible through the use of metagenomic techniques and single-cell genomics. When used synergistically, these metagenomic and single-cell techniques create a powerful tool to study microbial diversity. These genomics techniques have already been successfully exploited to identify sources for i) novel enzymes or natural products for biotechnology applications, ii) novel genes from extremophiles, and iii) whole genomes or operons from uncultured microbes. More can be done to utilize these tools more efficiently in biotechnology.
KAUST Department:
Red Sea Research Center (RSRC)
Citation:
Contemporary molecular tools in microbial ecology and their application to advancing biotechnology 2015 Biotechnology Advances
Publisher:
Elsevier BV
Journal:
Biotechnology Advances
Issue Date:
25-Sep-2015
DOI:
10.1016/j.biotechadv.2015.09.005
Type:
Article
ISSN:
07349750
Additional Links:
http://linkinghub.elsevier.com/retrieve/pii/S0734975015300380
Appears in Collections:
Articles; Red Sea Research Center (RSRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorRashid, Mamoonen
dc.contributor.authorStingl, Ulrichen
dc.date.accessioned2015-09-29T06:52:42Zen
dc.date.available2015-09-29T06:52:42Zen
dc.date.issued2015-09-25en
dc.identifier.citationContemporary molecular tools in microbial ecology and their application to advancing biotechnology 2015 Biotechnology Advancesen
dc.identifier.issn07349750en
dc.identifier.doi10.1016/j.biotechadv.2015.09.005en
dc.identifier.urihttp://hdl.handle.net/10754/578848en
dc.description.abstractNovel methods in microbial ecology are revolutionizing our understanding of the structure and function of microbes in the environment, but concomitant advances in applications of these tools to biotechnology are mostly lagging behind. After more than a century of efforts to improve microbial culturing techniques, about 70–80% of microbial diversity – recently called the “microbial dark matter” – remains uncultured. In early attempts to identify and sample these so far uncultured taxonomic lineages, methods that amplify and sequence ribosomal RNA genes were extensively used. Recent developments in cell separation techniques, DNA amplification, and high-throughput DNA sequencing platforms have now made the discovery of genes/genomes of uncultured microorganisms from different environments possible through the use of metagenomic techniques and single-cell genomics. When used synergistically, these metagenomic and single-cell techniques create a powerful tool to study microbial diversity. These genomics techniques have already been successfully exploited to identify sources for i) novel enzymes or natural products for biotechnology applications, ii) novel genes from extremophiles, and iii) whole genomes or operons from uncultured microbes. More can be done to utilize these tools more efficiently in biotechnology.en
dc.language.isoenen
dc.publisherElsevier BVen
dc.relation.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0734975015300380en
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Biotechnology Advances. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biotechnology Advances, 25 September 2015. DOI: 10.1016/j.biotechadv.2015.09.005en
dc.subjectEcologyen
dc.subjectBiotechnologyen
dc.subjectMetagenomicsen
dc.subjectSingle-cell genomicsen
dc.subjectNatural productsen
dc.subjectEnzymesen
dc.titleContemporary molecular tools in microbial ecology and their application to advancing biotechnologyen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalBiotechnology Advancesen
dc.eprint.versionPost-printen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorRashid, Mamoonen
kaust.authorStingl, Ulrichen
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