Metatranscriptome analysis of the reef-building coral Orbicella faveolata indicates holobiont response to coral disease

Handle URI:
http://hdl.handle.net/10754/577334
Title:
Metatranscriptome analysis of the reef-building coral Orbicella faveolata indicates holobiont response to coral disease
Authors:
Daniels, Camille Arian ( 0000-0002-5227-5394 ) ; Baumgarten, Sebastian ( 0000-0003-2646-7699 ) ; Yum, Lauren ( 0000-0003-2439-561X ) ; Michell, Craig; Bayer, Till; Arif, Chatchanit ( 0000-0001-5640-9970 ) ; Roder, Cornelia; Weil, Ernesto; Voolstra, Christian R. ( 0000-0003-4555-3795 )
Abstract:
White Plague Disease (WPD) is implicated in coral reef decline in the Caribbean and is characterized by microbial community shifts in coral mucus and tissue. Studies thus far have focused on assessing microbial communities or the identification of specific pathogens, yet few have addressed holobiont response across metaorganism compartments in coral disease. Here, we report on the first metatranscriptomic assessment of the coral host, algal symbiont, and microbial compartment in order to survey holobiont structure and function in healthy and diseased samples from Orbicella faveolata collected at reef sites off Puerto Rico. Our data indicate holobiont-wide as well as compartment-specific responses to WPD. Gene expression changes in the diseased coral host involved proteins playing a role in innate immunity, cytoskeletal integrity, cell adhesion, oxidative stress, chemical defense, and retroelements. In contrast, the algal symbiont showed comparatively few expression changes, but of large magnitude, of genes related to stress, photosynthesis, and metal transport. Concordant with the coral host response, the bacterial compartment showed increased abundance of heat shock proteins, genes related to oxidative stress, DNA repair, and potential retroelement activity. Importantly, analysis of the expressed bacterial gene functions establishes the participation of multiple bacterial families in WPD pathogenesis and also suggests a possible involvement of viruses and/or phages in structuring the bacterial assemblage. In this study, we implement an experimental approach to partition the coral holobiont and resolve compartment- and taxa-specific responses in order to understand metaorganism function in coral disease.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Red Sea Research Center (RSRC)
Citation:
Metatranscriptome analysis of the reef-building coral Orbicella faveolata indicates holobiont response to coral disease 2015, 2 Frontiers in Marine Science
Publisher:
Frontiers Media SA
Journal:
Frontiers in Marine Science
Issue Date:
11-Sep-2015
DOI:
10.3389/fmars.2015.00062
Type:
Article
ISSN:
2296-7745
Additional Links:
http://journal.frontiersin.org/article/10.3389/fmars.2015.00062
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDaniels, Camille Arianen
dc.contributor.authorBaumgarten, Sebastianen
dc.contributor.authorYum, Laurenen
dc.contributor.authorMichell, Craigen
dc.contributor.authorBayer, Tillen
dc.contributor.authorArif, Chatchaniten
dc.contributor.authorRoder, Corneliaen
dc.contributor.authorWeil, Ernestoen
dc.contributor.authorVoolstra, Christian R.en
dc.date.accessioned2015-09-15T12:39:48Zen
dc.date.available2015-09-15T12:39:48Zen
dc.date.issued2015-09-11en
dc.identifier.citationMetatranscriptome analysis of the reef-building coral Orbicella faveolata indicates holobiont response to coral disease 2015, 2 Frontiers in Marine Scienceen
dc.identifier.issn2296-7745en
dc.identifier.doi10.3389/fmars.2015.00062en
dc.identifier.urihttp://hdl.handle.net/10754/577334en
dc.description.abstractWhite Plague Disease (WPD) is implicated in coral reef decline in the Caribbean and is characterized by microbial community shifts in coral mucus and tissue. Studies thus far have focused on assessing microbial communities or the identification of specific pathogens, yet few have addressed holobiont response across metaorganism compartments in coral disease. Here, we report on the first metatranscriptomic assessment of the coral host, algal symbiont, and microbial compartment in order to survey holobiont structure and function in healthy and diseased samples from Orbicella faveolata collected at reef sites off Puerto Rico. Our data indicate holobiont-wide as well as compartment-specific responses to WPD. Gene expression changes in the diseased coral host involved proteins playing a role in innate immunity, cytoskeletal integrity, cell adhesion, oxidative stress, chemical defense, and retroelements. In contrast, the algal symbiont showed comparatively few expression changes, but of large magnitude, of genes related to stress, photosynthesis, and metal transport. Concordant with the coral host response, the bacterial compartment showed increased abundance of heat shock proteins, genes related to oxidative stress, DNA repair, and potential retroelement activity. Importantly, analysis of the expressed bacterial gene functions establishes the participation of multiple bacterial families in WPD pathogenesis and also suggests a possible involvement of viruses and/or phages in structuring the bacterial assemblage. In this study, we implement an experimental approach to partition the coral holobiont and resolve compartment- and taxa-specific responses in order to understand metaorganism function in coral disease.en
dc.language.isoenen
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fmars.2015.00062en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. http://creativecommons.org/licenses/by/4.0/en
dc.subjectcoral reefen
dc.subjectcoral diseaseen
dc.subjectmetatranscriptomicsen
dc.subjectSymbiodiniumen
dc.subjectmetaorganismen
dc.titleMetatranscriptome analysis of the reef-building coral Orbicella faveolata indicates holobiont response to coral diseaseen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.identifier.journalFrontiers in Marine Scienceen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionGEOMAR Department: Evolutionary Ecology of Marine Fishes, GEOMAR Helmholtz Centre for Ocean Research, Kiel, Germanyen
dc.contributor.institutionDepartment of Marine Sciences, University of Puerto Rico, Mayaguez, Puerto Ricoen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDaniels, Camille Arianen
kaust.authorBaumgarten, Sebastianen
kaust.authorYum, Laurenen
kaust.authorMichell, Craigen
kaust.authorBayer, Tillen
kaust.authorArif, Chatchaniten
kaust.authorRoder, Corneliaen
kaust.authorVoolstra, Christian R.en
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