Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes

Handle URI:
http://hdl.handle.net/10754/577333
Title:
Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes
Authors:
Sghaier, Haïtham; Hezbri, Karima ( 0000-0001-8693-2316 ) ; Ghodhbane-Gtari, Faten; Pujic, Petar; Sen, Arnab; Daffonchio, Daniele ( 0000-0003-0947-925X ) ; Boudabous, Abdellatif; Tisa, Louis S; Klenk, Hans-Peter; Armengaud, Jean; Normand, Philippe; Gtari, Maher ( 0000-0001-6946-6907 )
Abstract:
The Geodermatophilaceae are unique model systems to study the ability to thrive on or within stones and their proteogenomes (referring to the whole protein arsenal encoded by the genome) could provide important insight into their adaptation mechanisms. Here we report the detailed comparative genome analysis of Blastococcus saxobsidens (Bs), Modestobacter marinus (Mm) and Geodermatophilus obscurus (Go) isolated respectively from the interior and the surface of calcarenite stones and from desert sandy soils. The genome-scale analysis of Bs, Mm and Go illustrates how adaptation to these niches can be achieved through various strategies including ‘molecular tinkering/opportunism’ as shown by the high proportion of lost, duplicated or horizontally transferred genes and ORFans. Using high-throughput discovery proteomics, the three proteomes under unstressed conditions were analyzed, highlighting the most abundant biomarkers and the main protein factors. Proteomic data corroborated previously demonstrated stone-related ecological distribution. For instance, these data showed starvation-inducible, biofilm-related and DNA-protection proteins as signatures of the microbes associated with the interior, surface and outside of stones, respectively.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes 2015 The ISME Journal
Publisher:
Nature Publishing Group
Journal:
The ISME Journal
Issue Date:
30-Jun-2015
DOI:
10.1038/ismej.2015.108
Type:
Article
ISSN:
1751-7362; 1751-7370
Additional Links:
http://www.nature.com/doifinder/10.1038/ismej.2015.108
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorSghaier, Haïthamen
dc.contributor.authorHezbri, Karimaen
dc.contributor.authorGhodhbane-Gtari, Fatenen
dc.contributor.authorPujic, Petaren
dc.contributor.authorSen, Arnaben
dc.contributor.authorDaffonchio, Danieleen
dc.contributor.authorBoudabous, Abdellatifen
dc.contributor.authorTisa, Louis Sen
dc.contributor.authorKlenk, Hans-Peteren
dc.contributor.authorArmengaud, Jeanen
dc.contributor.authorNormand, Philippeen
dc.contributor.authorGtari, Maheren
dc.date.accessioned2015-09-16T07:18:36Zen
dc.date.available2015-09-16T07:18:36Zen
dc.date.issued2015-06-30en
dc.identifier.citationStone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes 2015 The ISME Journalen
dc.identifier.issn1751-7362en
dc.identifier.issn1751-7370en
dc.identifier.doi10.1038/ismej.2015.108en
dc.identifier.urihttp://hdl.handle.net/10754/577333en
dc.description.abstractThe Geodermatophilaceae are unique model systems to study the ability to thrive on or within stones and their proteogenomes (referring to the whole protein arsenal encoded by the genome) could provide important insight into their adaptation mechanisms. Here we report the detailed comparative genome analysis of Blastococcus saxobsidens (Bs), Modestobacter marinus (Mm) and Geodermatophilus obscurus (Go) isolated respectively from the interior and the surface of calcarenite stones and from desert sandy soils. The genome-scale analysis of Bs, Mm and Go illustrates how adaptation to these niches can be achieved through various strategies including ‘molecular tinkering/opportunism’ as shown by the high proportion of lost, duplicated or horizontally transferred genes and ORFans. Using high-throughput discovery proteomics, the three proteomes under unstressed conditions were analyzed, highlighting the most abundant biomarkers and the main protein factors. Proteomic data corroborated previously demonstrated stone-related ecological distribution. For instance, these data showed starvation-inducible, biofilm-related and DNA-protection proteins as signatures of the microbes associated with the interior, surface and outside of stones, respectively.en
dc.language.isoenen
dc.publisherNature Publishing Groupen
dc.relation.urlhttp://www.nature.com/doifinder/10.1038/ismej.2015.108en
dc.rightsArchived with thanks to The ISME Journalen
dc.titleStone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalThe ISME Journalen
dc.eprint.versionPost-printen
dc.contributor.institutionNational Center for Nuclear Sciences and Technology, Sidi Thabet Technopark, Ariana, Tunisiaen
dc.contributor.institutionLaboratoire Microorganismes et Biomolécules Actives, Université de Tunis Elmanar (FST) and Université de Carthage (INSAT), Tunis, Tunisiaen
dc.contributor.institutionUniversité Lyon 1, Université de Lyon, CNRS, Ecologie Microbienne, UMR 5557, Villeurbannes, Franceen
dc.contributor.institutionNBU Bioinformatics Facility, Department of Botany, University of North Bengal, Siliguri, Indiaen
dc.contributor.institutionDepartment of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Milan, Italyen
dc.contributor.institutionDepartment of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH, USAen
dc.contributor.institutionSchool of Biology, Newcastle University, Newcastle upon Tyne, UKen
dc.contributor.institutionCEA, DSV, IBITEC-S, Lab Innovative Technologies for Detection and Diagnostic, Bagnols-sur-Cèze, Franceen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDaffonchio, Danieleen
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