Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA

Handle URI:
http://hdl.handle.net/10754/577061
Title:
Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA
Authors:
Sugumar, Thennarasu; Harishankar, M.; Dhinakar Raj, G.
Abstract:
Full-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.
KAUST Department:
Biosciences Core Lab
Publisher:
Wiley-Blackwell
Journal:
International Journal of Immunogenetics
Issue Date:
12-Dec-2011
DOI:
10.1111/j.1744-313X.2011.01075.x
Type:
Article
ISSN:
1744-3121
Sponsors:
The authors thank the Department of Biotechnology (DBT), Government of India, for financial assistance to this Project. ST and MHS were SRFs who worked in this project.
Appears in Collections:
Articles; Biosciences Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorSugumar, Thennarasuen
dc.contributor.authorHarishankar, M.en
dc.contributor.authorDhinakar Raj, G.en
dc.date.accessioned2015-09-10T09:28:07Zen
dc.date.available2015-09-10T09:28:07Zen
dc.date.issued2011-12-12en
dc.identifier.issn1744-3121en
dc.identifier.doi10.1111/j.1744-313X.2011.01075.xen
dc.identifier.urihttp://hdl.handle.net/10754/577061en
dc.description.abstractFull-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.en
dc.description.sponsorshipThe authors thank the Department of Biotechnology (DBT), Government of India, for financial assistance to this Project. ST and MHS were SRFs who worked in this project.en
dc.publisherWiley-Blackwellen
dc.titleCloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNAen
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalInternational Journal of Immunogeneticsen
dc.contributor.institutionTamil Nadu Vet & Anim Sci Univ, Dept Anim Biotechnol, Madras Vet Coll, Madras, Tamil Nadu, Indiaen
kaust.authorSugumar, Thennarasuen
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