In vitro regeneration from petiole explants of non-toxic Jatropha curcas

Handle URI:
http://hdl.handle.net/10754/577058
Title:
In vitro regeneration from petiole explants of non-toxic Jatropha curcas
Authors:
Kumar, Nitish; Vijay Anand, K.G.; Reddy, Muppala P.
Abstract:
Jatropha curcas, a multipurpose shrub has acquired significant economic potential as biodiesel plant. The seeds or pressed cake is toxic due to the presence of toxic substances and is not useful as food/fodder despite having the best protein composition. A simple, efficient, and reproducible method for plant regeneration through direct organogenesis from petiole explants of non-toxic J. curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ). The best induction of shoot buds (57.61%), and number of shoot buds (4.98) per explant were obtained when in vitro petiole explants were placed horizontally on MS medium supplemented with 2.27 mu M TDZ. The Induced shoot buds were transferred to MS medium containing 10 mu M kinetin (Kn), 4.5 mu M 6-benzyl aminopurine (BA), and 5.5 mu M alpha-naphthaleneacetic acid (NAA) for shoot proliferation and subsequent elongation was achieved on MS medium supplemented with 2.25 mu M BA and 8.5 mu M IAA. The elongated shoots could be rooted on half-strength MS medium with 15 mu M IBA, 11.4 mu M IAA and 5.5 mu M NAA with more than 90% survival rate. (C) 2010 Elsevier B.V. All rights reserved.
KAUST Department:
Center for Desert Agriculture
Publisher:
Elsevier BV
Journal:
Industrial Crops and Products
Issue Date:
Jan-2011
DOI:
10.1016/j.indcrop.2010.09.013
Type:
Article
ISSN:
0926-6690
Sponsors:
The authors gratefully acknowledge Council of Scientific and Industrial Research, New Delhi for financial assistance and Prof. K. Becker, Department of Aquaculture Systems and Animal Nutrition, University of Hohenheim, Stuttgart, Germany for providing Mexican non-toxic J. curcas seeds.
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorKumar, Nitishen
dc.contributor.authorVijay Anand, K.G.en
dc.contributor.authorReddy, Muppala P.en
dc.date.accessioned2015-09-10T09:28:02Zen
dc.date.available2015-09-10T09:28:02Zen
dc.date.issued2011-01en
dc.identifier.issn0926-6690en
dc.identifier.doi10.1016/j.indcrop.2010.09.013en
dc.identifier.urihttp://hdl.handle.net/10754/577058en
dc.description.abstractJatropha curcas, a multipurpose shrub has acquired significant economic potential as biodiesel plant. The seeds or pressed cake is toxic due to the presence of toxic substances and is not useful as food/fodder despite having the best protein composition. A simple, efficient, and reproducible method for plant regeneration through direct organogenesis from petiole explants of non-toxic J. curcas was developed using Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ). The best induction of shoot buds (57.61%), and number of shoot buds (4.98) per explant were obtained when in vitro petiole explants were placed horizontally on MS medium supplemented with 2.27 mu M TDZ. The Induced shoot buds were transferred to MS medium containing 10 mu M kinetin (Kn), 4.5 mu M 6-benzyl aminopurine (BA), and 5.5 mu M alpha-naphthaleneacetic acid (NAA) for shoot proliferation and subsequent elongation was achieved on MS medium supplemented with 2.25 mu M BA and 8.5 mu M IAA. The elongated shoots could be rooted on half-strength MS medium with 15 mu M IBA, 11.4 mu M IAA and 5.5 mu M NAA with more than 90% survival rate. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipThe authors gratefully acknowledge Council of Scientific and Industrial Research, New Delhi for financial assistance and Prof. K. Becker, Department of Aquaculture Systems and Animal Nutrition, University of Hohenheim, Stuttgart, Germany for providing Mexican non-toxic J. curcas seeds.en
dc.publisherElsevier BVen
dc.titleIn vitro regeneration from petiole explants of non-toxic Jatropha curcasen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalIndustrial Crops and Productsen
dc.contributor.institutionCSIR, Cent Salt & Marine Chem Res Inst, Discipline Wasteland Res, Bhavnagar 364002, Gujarat, Indiaen
kaust.authorReddy, Muppala P.en
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