The structure of DNA by direct imaging

Handle URI:
http://hdl.handle.net/10754/576450
Title:
The structure of DNA by direct imaging
Authors:
Marini, Monica ( 0000-0001-8182-5239 ) ; Falqui, Andrea ( 0000-0002-1476-7742 ) ; Moretti, Manola; Limongi, Tania; Allione, Marco ( 0000-0003-0757-9791 ) ; Genovese, Alessandro; Lopatin, Sergei; Tirinato, Luca ( 0000-0001-9826-2129 ) ; Das, Gobind ( 0000-0003-0942-681X ) ; Torre, Bruno; Giugni, Andrea; Gentile, Francesco; Candeloro, Patrizio; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
The structure of DNA was determined in 1953 by x-ray fiber diffraction. Several attempts have been made to obtain a direct image of DNA with alternative techniques. The direct image is intended to allow a quantitative evaluation of all relevant characteristic lengths present in a molecule. A direct image of DNA, which is different from diffraction in the reciprocal space, is difficult to obtain for two main reasons: the intrinsic very low contrast of the elements that form the molecule and the difficulty of preparing the sample while preserving its pristine shape and size. We show that through a preparation procedure compatible with the DNA physiological conditions, a direct image of a single suspended DNA molecule can be obtained. In the image, all relevant lengths of A-form DNA are measurable. A high-resolution transmission electron microscope that operates at 80 keV with an ultimate resolution of 1.5 Å was used for this experiment. Direct imaging of a single molecule can be used as a method to address biological problems that require knowledge at the single-molecule level, given that the average information obtained by x-ray diffraction of crystals or fibers is not sufficient for detailed structure determination, or when crystals cannot be obtained from biological molecules or are not sufficient in understanding multiple protein configurations.
KAUST Department:
SMILEs Lab; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division; NABLA Lab; Imaging and Characterization Core Lab
Citation:
The structure of DNA by direct imaging 2015, 1 (7):e1500734 Science Advances
Publisher:
American Association for the Advancement of Science (AAAS)
Journal:
Science Advances
Issue Date:
28-Aug-2015
DOI:
10.1126/sciadv.1500734
Type:
Article
ISSN:
2375-2548
Additional Links:
http://advances.sciencemag.org/cgi/doi/10.1126/sciadv.1500734
Appears in Collections:
Articles; Advanced Nanofabrication, Imaging and Characterization Core Lab; Physical Sciences and Engineering (PSE) Division; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMarini, Monicaen
dc.contributor.authorFalqui, Andreaen
dc.contributor.authorMoretti, Manolaen
dc.contributor.authorLimongi, Taniaen
dc.contributor.authorAllione, Marcoen
dc.contributor.authorGenovese, Alessandroen
dc.contributor.authorLopatin, Sergeien
dc.contributor.authorTirinato, Lucaen
dc.contributor.authorDas, Gobinden
dc.contributor.authorTorre, Brunoen
dc.contributor.authorGiugni, Andreaen
dc.contributor.authorGentile, Francescoen
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-09-03T12:50:20Zen
dc.date.available2015-09-03T12:50:20Zen
dc.date.issued2015-08-28en
dc.identifier.citationThe structure of DNA by direct imaging 2015, 1 (7):e1500734 Science Advancesen
dc.identifier.issn2375-2548en
dc.identifier.doi10.1126/sciadv.1500734en
dc.identifier.urihttp://hdl.handle.net/10754/576450en
dc.description.abstractThe structure of DNA was determined in 1953 by x-ray fiber diffraction. Several attempts have been made to obtain a direct image of DNA with alternative techniques. The direct image is intended to allow a quantitative evaluation of all relevant characteristic lengths present in a molecule. A direct image of DNA, which is different from diffraction in the reciprocal space, is difficult to obtain for two main reasons: the intrinsic very low contrast of the elements that form the molecule and the difficulty of preparing the sample while preserving its pristine shape and size. We show that through a preparation procedure compatible with the DNA physiological conditions, a direct image of a single suspended DNA molecule can be obtained. In the image, all relevant lengths of A-form DNA are measurable. A high-resolution transmission electron microscope that operates at 80 keV with an ultimate resolution of 1.5 Å was used for this experiment. Direct imaging of a single molecule can be used as a method to address biological problems that require knowledge at the single-molecule level, given that the average information obtained by x-ray diffraction of crystals or fibers is not sufficient for detailed structure determination, or when crystals cannot be obtained from biological molecules or are not sufficient in understanding multiple protein configurations.en
dc.language.isoenen
dc.publisherAmerican Association for the Advancement of Science (AAAS)en
dc.relation.urlhttp://advances.sciencemag.org/cgi/doi/10.1126/sciadv.1500734en
dc.rights2015 © The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). 10.1126/sciadv.1500734en
dc.titleThe structure of DNA by direct imagingen
dc.typeArticleen
dc.contributor.departmentSMILEs Laben
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentNABLA Laben
dc.contributor.departmentImaging and Characterization Core Laben
dc.identifier.journalScience Advancesen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionBio Nanotechnology and Engineering for Medicine (BIONEM), Department of Experimental and Clinical Medicine, University of Magna Graecia, Viale Europa, Germaneto, 88100 Catanzaro, Italyen
dc.contributor.institutionDepartment of Electrical Engineering and Information Technology, University of Naples, 80125 Naples, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorMarini, Monicaen
kaust.authorFalqui, Andreaen
kaust.authorMoretti, Manolaen
kaust.authorLimongi, Taniaen
kaust.authorAllione, Marcoen
kaust.authorGenovese, Alessandroen
kaust.authorLopatin, Sergeien
kaust.authorTirinato, Lucaen
kaust.authorDas, Gobinden
kaust.authorTorre, Brunoen
kaust.authorGiugni, Andreaen
kaust.authorDi Fabrizio, Enzo M.en
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