Selective phosphorylation during early macrophage differentiation

Handle URI:
http://hdl.handle.net/10754/576076
Title:
Selective phosphorylation during early macrophage differentiation
Authors:
Zhang, Huoming ( 0000-0001-5416-0358 ) ; Qian, Pei-Yuan; Ravasi, Timothy ( 0000-0002-9950-465X )
Abstract:
The differentiation of macrophages from monocytes is a tightly controlled and complex biological process. Although numerous studies have been conducted using biochemical approaches or global gene/gene profiling, the mechanisms of the early stages of differentiation remain unclear. Here we used SILAC-based quantitative proteomics approach to perform temporal phosphoproteome profiling of early macrophage differentiation. We identified a large set of phosphoproteins and grouped them as PMA-regulated and non-regulated phosphoproteins in the early stages of differentiation. Further analysis of the PMA-regulated phosphoproteins revealed that transcriptional suppression, cytoskeletal reorganization and cell adhesion were among the most significantly activated pathways. Some key involved regulators of these pathways are mTOR, MYB, STAT1 and CTNNB. Moreover, we were able to classify the roles and activities of several transcriptional factors during different differentiation stages and found that E2F is likely to be an important regulator during the relatively late stages of differentiation. This study provides the first comprehensive picture of the dynamic phosphoproteome during myeloid cells differentiation, and identifies potential molecular targets in leukemic cells.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Applied Mathematics and Computational Science Program; Biosciences Core Lab
Citation:
Selective phosphorylation during early macrophage differentiation 2015:n/a PROTEOMICS
Publisher:
Wiley-Blackwell
Journal:
PROTEOMICS
Issue Date:
26-Aug-2015
DOI:
10.1002/pmic.201400511
Type:
Article
ISSN:
16159853
Additional Links:
http://doi.wiley.com/10.1002/pmic.201400511
Appears in Collections:
Articles; Biosciences Core Lab; Applied Mathematics and Computational Science Program; Biological and Environmental Sciences and Engineering (BESE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Huomingen
dc.contributor.authorQian, Pei-Yuanen
dc.contributor.authorRavasi, Timothyen
dc.date.accessioned2015-08-30T11:16:28Zen
dc.date.available2015-08-30T11:16:28Zen
dc.date.issued2015-08-26en
dc.identifier.citationSelective phosphorylation during early macrophage differentiation 2015:n/a PROTEOMICSen
dc.identifier.issn16159853en
dc.identifier.doi10.1002/pmic.201400511en
dc.identifier.urihttp://hdl.handle.net/10754/576076en
dc.description.abstractThe differentiation of macrophages from monocytes is a tightly controlled and complex biological process. Although numerous studies have been conducted using biochemical approaches or global gene/gene profiling, the mechanisms of the early stages of differentiation remain unclear. Here we used SILAC-based quantitative proteomics approach to perform temporal phosphoproteome profiling of early macrophage differentiation. We identified a large set of phosphoproteins and grouped them as PMA-regulated and non-regulated phosphoproteins in the early stages of differentiation. Further analysis of the PMA-regulated phosphoproteins revealed that transcriptional suppression, cytoskeletal reorganization and cell adhesion were among the most significantly activated pathways. Some key involved regulators of these pathways are mTOR, MYB, STAT1 and CTNNB. Moreover, we were able to classify the roles and activities of several transcriptional factors during different differentiation stages and found that E2F is likely to be an important regulator during the relatively late stages of differentiation. This study provides the first comprehensive picture of the dynamic phosphoproteome during myeloid cells differentiation, and identifies potential molecular targets in leukemic cells.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.relation.urlhttp://doi.wiley.com/10.1002/pmic.201400511en
dc.rightsThis is the peer reviewed version of the following article: Zhang, Huoming, Pei‐Yuan Qian, and Timothy Ravasi. "Selective phosphorylation during early macrophage differentiation." PROTEOMICS (2015)., which has been published in final form at http://doi.wiley.com/10.1002/pmic.201400511. This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.en
dc.subjectLeukemiaen
dc.subjectMonocyte-to-macrophage differentiationen
dc.subjectPhosphoproteomeen
dc.titleSelective phosphorylation during early macrophage differentiationen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalPROTEOMICSen
dc.eprint.versionPost-printen
dc.contributor.institutionSchool of Science; Hong Kong University of Science and Technology; Hong Kong Chinaen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorZhang, Huomingen
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