Single-Molecule Imaging Reveals Topology Dependent Mutual Relaxation of Polymer Chains

Handle URI:
http://hdl.handle.net/10754/576074
Title:
Single-Molecule Imaging Reveals Topology Dependent Mutual Relaxation of Polymer Chains
Authors:
Abadi, Maram ( 0000-0003-3802-8669 ) ; Serag, Maged F. ( 0000-0002-6153-1089 ) ; Habuchi, Satoshi ( 0000-0002-6663-2807 )
Abstract:
The motion and relaxation of linear and cyclic polymers under entangled conditions are investigated by means of a newly developed single-molecule tracking technique, cumulative-area (CA) tracking. CA tracking enables simultaneous quantitative characterization of the diffusion mode, diffusion rate, and relaxation time that have been impossible with a widely used conventional single-molecule localization and tracking method, by analyzing cumulative areas occupied by the moving molecule. Using the novel approach, we investigate the motion and relaxation of entangled cyclic polymers, which have been an important but poorly understood question. Fluorescently labeled 42 kbp linear or cyclic tracer dsDNAs in concentrated solutions of unlabeled linear or cyclic DNAs are used as model systems. We show that CA tracking can explicitly distinguish topology-dependent diffusion mode, rate, and relaxation time, demonstrating that the method provides an invaluable tool for characterizing topological interaction between the entangled chains. We further demonstrate that the current models proposed for the entanglement between cyclic polymers which are based on cyclic chains moving through an array of fixed obstacles cannot correctly describe the motion of the cyclic chain under the entangled conditions. Our results rather suggest the mutual relaxation of the cyclic chains, which underscore the necessity of developing a new model to describe the motion of cyclic polymer under the entangled conditions based on the mutual interaction of the chains.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Single-Molecule Imaging Reveals Topology Dependent Mutual Relaxation of Polymer Chains 2015:150824084240002 Macromolecules
Publisher:
American Chemical Society (ACS)
Journal:
Macromolecules
Issue Date:
24-Aug-2015
DOI:
10.1021/acs.macromol.5b01388
Type:
Article
ISSN:
0024-9297; 1520-5835
Additional Links:
http://pubs.acs.org/doi/10.1021/acs.macromol.5b01388
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAbadi, Maramen
dc.contributor.authorSerag, Maged F.en
dc.contributor.authorHabuchi, Satoshien
dc.date.accessioned2015-08-30T11:10:48Zen
dc.date.available2015-08-30T11:10:48Zen
dc.date.issued2015-08-24en
dc.identifier.citationSingle-Molecule Imaging Reveals Topology Dependent Mutual Relaxation of Polymer Chains 2015:150824084240002 Macromoleculesen
dc.identifier.issn0024-9297en
dc.identifier.issn1520-5835en
dc.identifier.doi10.1021/acs.macromol.5b01388en
dc.identifier.urihttp://hdl.handle.net/10754/576074en
dc.description.abstractThe motion and relaxation of linear and cyclic polymers under entangled conditions are investigated by means of a newly developed single-molecule tracking technique, cumulative-area (CA) tracking. CA tracking enables simultaneous quantitative characterization of the diffusion mode, diffusion rate, and relaxation time that have been impossible with a widely used conventional single-molecule localization and tracking method, by analyzing cumulative areas occupied by the moving molecule. Using the novel approach, we investigate the motion and relaxation of entangled cyclic polymers, which have been an important but poorly understood question. Fluorescently labeled 42 kbp linear or cyclic tracer dsDNAs in concentrated solutions of unlabeled linear or cyclic DNAs are used as model systems. We show that CA tracking can explicitly distinguish topology-dependent diffusion mode, rate, and relaxation time, demonstrating that the method provides an invaluable tool for characterizing topological interaction between the entangled chains. We further demonstrate that the current models proposed for the entanglement between cyclic polymers which are based on cyclic chains moving through an array of fixed obstacles cannot correctly describe the motion of the cyclic chain under the entangled conditions. Our results rather suggest the mutual relaxation of the cyclic chains, which underscore the necessity of developing a new model to describe the motion of cyclic polymer under the entangled conditions based on the mutual interaction of the chains.en
dc.language.isoenen
dc.publisherAmerican Chemical Society (ACS)en
dc.relation.urlhttp://pubs.acs.org/doi/10.1021/acs.macromol.5b01388en
dc.rightsArchived with thanks to Macromoleculesen
dc.titleSingle-Molecule Imaging Reveals Topology Dependent Mutual Relaxation of Polymer Chainsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMacromoleculesen
dc.eprint.versionPost-printen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorSerag, Maged F.en
kaust.authorHabuchi, Satoshien
kaust.authorAbadi, Maramen
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