Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop

Handle URI:
http://hdl.handle.net/10754/575904
Title:
Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop
Authors:
Gedye, Kristene; Gonzalez-Hernandez, Jose; Ban, Yuguang; Ge, Xijin; Thimmapuram, Jyothi; Sun, Fengjie; Wright, Chris; Ali, Shahjahan; Boe, Arvid; Owens, Vance
Abstract:
Prairie cordgrass (Spartina pectinata Bosc ex Link) is being developed as a cellulosic biomass crop. Development of this species will require numerous steps, including breeding, agronomy, and characterization of the species genome. The research in this paper describes the first investigation of the transcriptome of prairie cordgrass via Next Generation Sequencing Technology, 454 GS FLX. A total of 556,198 expressed sequence tags (ESTs) were produced from four prairie cordgrass tissues: roots, rhizomes, immature inflorescence, and hooks. These ESTs were assembled into 26,302 contigs and 71,103 singletons. From these data were identified, EST-SSR (simple sequence repeat) regions and cell wall biosynthetic pathway genes suitable for the development of molecular markers which can aid the breeding process of prairie cordgrass by means of marker assisted selection.
KAUST Department:
Core Labs
Publisher:
Crop Science Society of America
Journal:
The Plant Genome Journal
Issue Date:
15-Sep-2010
DOI:
10.3835/plantgenome2010.06.0012
Type:
Article
ISSN:
1940-3372
Sponsors:
The authors acknowledge support from the Joint USDA-DOE Feedstock Genomics program (grant# 2007-35504-18236), the Center of Excellence in Drought Tolerance through the South Dakota 2010 Initiative, and the South Dakota Agri. Exp. Station.
Appears in Collections:
Articles; Biosciences Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorGedye, Kristeneen
dc.contributor.authorGonzalez-Hernandez, Joseen
dc.contributor.authorBan, Yuguangen
dc.contributor.authorGe, Xijinen
dc.contributor.authorThimmapuram, Jyothien
dc.contributor.authorSun, Fengjieen
dc.contributor.authorWright, Chrisen
dc.contributor.authorAli, Shahjahanen
dc.contributor.authorBoe, Arviden
dc.contributor.authorOwens, Vanceen
dc.date.accessioned2015-08-25T06:18:54Zen
dc.date.available2015-08-25T06:18:54Zen
dc.date.issued2010-09-15en
dc.identifier.issn1940-3372en
dc.identifier.doi10.3835/plantgenome2010.06.0012en
dc.identifier.urihttp://hdl.handle.net/10754/575904en
dc.description.abstractPrairie cordgrass (Spartina pectinata Bosc ex Link) is being developed as a cellulosic biomass crop. Development of this species will require numerous steps, including breeding, agronomy, and characterization of the species genome. The research in this paper describes the first investigation of the transcriptome of prairie cordgrass via Next Generation Sequencing Technology, 454 GS FLX. A total of 556,198 expressed sequence tags (ESTs) were produced from four prairie cordgrass tissues: roots, rhizomes, immature inflorescence, and hooks. These ESTs were assembled into 26,302 contigs and 71,103 singletons. From these data were identified, EST-SSR (simple sequence repeat) regions and cell wall biosynthetic pathway genes suitable for the development of molecular markers which can aid the breeding process of prairie cordgrass by means of marker assisted selection.en
dc.description.sponsorshipThe authors acknowledge support from the Joint USDA-DOE Feedstock Genomics program (grant# 2007-35504-18236), the Center of Excellence in Drought Tolerance through the South Dakota 2010 Initiative, and the South Dakota Agri. Exp. Station.en
dc.publisherCrop Science Society of Americaen
dc.titleInvestigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Cropen
dc.typeArticleen
dc.contributor.departmentCore Labsen
dc.identifier.journalThe Plant Genome Journalen
dc.contributor.institutionS Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USAen
dc.contributor.institutionS Dakota State Univ, Dep Math & Stat, Brookings, SD 57007 USAen
dc.contributor.institutionUniv Illinois, WM Keck Ctr Comparat & Funct Genom, Roy J Carver Biotechnol Ctr, Urbana, IL 61801 USAen
kaust.authorAli, Shahjahanen
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