Characterization and expression of calmodulin gene during larval settlement and metamorphosis of the polychaete Hydroides elegans

Handle URI:
http://hdl.handle.net/10754/575689
Title:
Characterization and expression of calmodulin gene during larval settlement and metamorphosis of the polychaete Hydroides elegans
Authors:
Chen, Zhangfan; Wang, Hao; Qian, Peiyuan
Abstract:
The polychaete . Hydroides elegans (Serpulidae, Lophotrochozoa) is a problematic marine fouling organism in most tropical and subtropical coastal environment. Competent larvae of . H. elegans undergo the transition from the swimming larval stage to the sessile juvenile stage with substantial morphological, physiological, and behavior changes. This transition is often referred to as larval settlement and metamorphosis. In this study, we examined the possible involvement of calmodulin (CaM) - a multifunctional calcium metabolism regulator, in the larval settlement and metamorphosis of . H. elegans. A full-length . CaM cDNA was successfully cloned from . H. elegans (. He-CaM) and it contained an open reading frame of 450. bp, encoding 149 amino acid residues. It was highly expressed in 12. h post-metamorphic juveniles, and remained high in adults. . In situ hybridization conducted in competent larvae and juveniles revealed that . He-CaM gene was continuously expressed in the putative growth zones, branchial rudiments, and collar region, suggesting that . He-CaM might be involved in tissue differentiation and development. Our subsequent bioassay revealed that the CaM inhibitor W7 could effectively inhibit larval settlement and metamorphosis, and cause some morphological defects of unsettled larvae. In conclusion, our results revealed that CaM has important functions in the larval settlement and metamorphosis of . H. elegans. © 2012 Elsevier Inc..
KAUST Department:
KAUST Global Collaborative Research Program; Materials Science and Engineering Program
Publisher:
Elsevier BV
Journal:
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Issue Date:
Aug-2012
DOI:
10.1016/j.cbpb.2012.04.001
PubMed ID:
22507549
Type:
Article
ISSN:
10964959
Sponsors:
We thank Dr. Yue Him Wong and Dr. Yu Zhang for their help with the sample collection; Dr. Kai He for technical assistance; Prof. Louis A Gosselin and Dr. Kiyokata Matsumura for assistance with revising the paper; and Fan Zhang, Dr. Shawn Arellano, and other lab members for their technical advice and constructive discussions. This study was supported by an award SA-C0040/UK-C0016 from the King Abdullah University of Science and Technology and grants (N_HKUST602/09 and AoE/P-04/04-2-II) from the Research Grants Council (RGC) of the Hong Kong SAR to PY Qian.
Appears in Collections:
Articles; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Zhangfanen
dc.contributor.authorWang, Haoen
dc.contributor.authorQian, Peiyuanen
dc.date.accessioned2015-08-24T08:35:54Zen
dc.date.available2015-08-24T08:35:54Zen
dc.date.issued2012-08en
dc.identifier.issn10964959en
dc.identifier.pmid22507549en
dc.identifier.doi10.1016/j.cbpb.2012.04.001en
dc.identifier.urihttp://hdl.handle.net/10754/575689en
dc.description.abstractThe polychaete . Hydroides elegans (Serpulidae, Lophotrochozoa) is a problematic marine fouling organism in most tropical and subtropical coastal environment. Competent larvae of . H. elegans undergo the transition from the swimming larval stage to the sessile juvenile stage with substantial morphological, physiological, and behavior changes. This transition is often referred to as larval settlement and metamorphosis. In this study, we examined the possible involvement of calmodulin (CaM) - a multifunctional calcium metabolism regulator, in the larval settlement and metamorphosis of . H. elegans. A full-length . CaM cDNA was successfully cloned from . H. elegans (. He-CaM) and it contained an open reading frame of 450. bp, encoding 149 amino acid residues. It was highly expressed in 12. h post-metamorphic juveniles, and remained high in adults. . In situ hybridization conducted in competent larvae and juveniles revealed that . He-CaM gene was continuously expressed in the putative growth zones, branchial rudiments, and collar region, suggesting that . He-CaM might be involved in tissue differentiation and development. Our subsequent bioassay revealed that the CaM inhibitor W7 could effectively inhibit larval settlement and metamorphosis, and cause some morphological defects of unsettled larvae. In conclusion, our results revealed that CaM has important functions in the larval settlement and metamorphosis of . H. elegans. © 2012 Elsevier Inc..en
dc.description.sponsorshipWe thank Dr. Yue Him Wong and Dr. Yu Zhang for their help with the sample collection; Dr. Kai He for technical assistance; Prof. Louis A Gosselin and Dr. Kiyokata Matsumura for assistance with revising the paper; and Fan Zhang, Dr. Shawn Arellano, and other lab members for their technical advice and constructive discussions. This study was supported by an award SA-C0040/UK-C0016 from the King Abdullah University of Science and Technology and grants (N_HKUST602/09 and AoE/P-04/04-2-II) from the Research Grants Council (RGC) of the Hong Kong SAR to PY Qian.en
dc.publisherElsevier BVen
dc.subjectCalmodulinen
dc.subjectGene expression patternen
dc.subjectLarval settlementen
dc.subjectPolychaeteen
dc.titleCharacterization and expression of calmodulin gene during larval settlement and metamorphosis of the polychaete Hydroides elegansen
dc.typeArticleen
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalComparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biologyen
kaust.authorWang, Haoen
kaust.authorChen, Zhangfanen
kaust.authorQian, Peiyuanen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.