Poly-ethers from Winogradskyella poriferorum: Antifouling potential, time-course study of production and natural abundance

Handle URI:
http://hdl.handle.net/10754/575681
Title:
Poly-ethers from Winogradskyella poriferorum: Antifouling potential, time-course study of production and natural abundance
Authors:
Dash, Swagatika; Nogata, Yasuyuki; Zhou, Xiaojian; Zhang, Yifan; Xü, Ying; Guo, Xian Rong; Zhang, Xixiang ( 0000-0002-3478-6414 ) ; Qian, Peiyuan
Abstract:
A sponge-associated bacterium, Winogradskyella poriferorum strain UST030701-295T was cultured up to 100l for extraction of antifouling bioactive compounds. Five poly-ethers were isolated and partially characterized based on nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS); two of them showed inhibitory effects on biofilm formation of marine bacteria and larval settlement of macro-foulers but did not produce any adverse effects on the phenotypes of zebra fish embryos at a concentration of 5μgml -1. The effect of culture duration on the production of the poly-ethers and the bioactivity of the relevant extracts was monitored over a period of 12days. The total crude poly-ether production increased from day 2 to day 5 and the highest bioactivity was observed on day 3. The poly-ethers were found to be localized in the cellular fraction of the extracts, implying their natural occurrence. The potent bioactivity of these poly-ethers together with their high natural abundance in bacteria makes them promising candidates as ingredients in antifouling applications. © 2011 Elsevier Ltd.
KAUST Department:
KAUST Global Collaborative Research Program; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program
Publisher:
Elsevier BV
Journal:
Bioresource Technology
Issue Date:
Aug-2011
DOI:
10.1016/j.biortech.2011.05.034
PubMed ID:
21664125
Type:
Article
ISSN:
09608524
Sponsors:
The authors thank Dr. Shawn Arellano and Ms. Cherry Kwan for proof reading and providing their valuable comments during the manuscript preparation. We also acknowledge Li Jie Sun (HKUST) and Yu Liang Yang (SIO, USA) for their technical assistance in NMR and MS data acquisition, Dr. Zilong Wen (HKUST) for providing the zebra fish embryo and Dr. Liu Xin for all the helpful discussions. This work was supported by the CAS/SAFEA International Partnership Program for Creative Research Teams KZCX2-YW-T001) and an award SA-C0040/UK-C0016 from the King Abdullah University of Science and Technology to Pei-Yuan Qian.
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorDash, Swagatikaen
dc.contributor.authorNogata, Yasuyukien
dc.contributor.authorZhou, Xiaojianen
dc.contributor.authorZhang, Yifanen
dc.contributor.authorXü, Yingen
dc.contributor.authorGuo, Xian Rongen
dc.contributor.authorZhang, Xixiangen
dc.contributor.authorQian, Peiyuanen
dc.date.accessioned2015-08-24T08:35:45Zen
dc.date.available2015-08-24T08:35:45Zen
dc.date.issued2011-08en
dc.identifier.issn09608524en
dc.identifier.pmid21664125en
dc.identifier.doi10.1016/j.biortech.2011.05.034en
dc.identifier.urihttp://hdl.handle.net/10754/575681en
dc.description.abstractA sponge-associated bacterium, Winogradskyella poriferorum strain UST030701-295T was cultured up to 100l for extraction of antifouling bioactive compounds. Five poly-ethers were isolated and partially characterized based on nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS); two of them showed inhibitory effects on biofilm formation of marine bacteria and larval settlement of macro-foulers but did not produce any adverse effects on the phenotypes of zebra fish embryos at a concentration of 5μgml -1. The effect of culture duration on the production of the poly-ethers and the bioactivity of the relevant extracts was monitored over a period of 12days. The total crude poly-ether production increased from day 2 to day 5 and the highest bioactivity was observed on day 3. The poly-ethers were found to be localized in the cellular fraction of the extracts, implying their natural occurrence. The potent bioactivity of these poly-ethers together with their high natural abundance in bacteria makes them promising candidates as ingredients in antifouling applications. © 2011 Elsevier Ltd.en
dc.description.sponsorshipThe authors thank Dr. Shawn Arellano and Ms. Cherry Kwan for proof reading and providing their valuable comments during the manuscript preparation. We also acknowledge Li Jie Sun (HKUST) and Yu Liang Yang (SIO, USA) for their technical assistance in NMR and MS data acquisition, Dr. Zilong Wen (HKUST) for providing the zebra fish embryo and Dr. Liu Xin for all the helpful discussions. This work was supported by the CAS/SAFEA International Partnership Program for Creative Research Teams KZCX2-YW-T001) and an award SA-C0040/UK-C0016 from the King Abdullah University of Science and Technology to Pei-Yuan Qian.en
dc.publisherElsevier BVen
dc.subjectAntifoulingen
dc.subjectBioactivityen
dc.subjectPoly-ethersen
dc.subjectPPGsen
dc.subjectWinogradskyella poriferorumen
dc.titlePoly-ethers from Winogradskyella poriferorum: Antifouling potential, time-course study of production and natural abundanceen
dc.typeArticleen
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalBioresource Technologyen
dc.contributor.institutionBiology Sector, Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry, Japanen
dc.contributor.institutionEnvironmental Science and Engineering Institute, Yangzhou University, Yangzhou, Chinaen
dc.contributor.institutionDepartment of Physics, Hong Kong University of Science and Technology, HKSAR, Chinaen
kaust.authorZhang, Xixiangen
kaust.authorDash, Swagatikaen
kaust.authorNogata, Yasuyukien
kaust.authorZhou, Xiaojianen
kaust.authorZhang, Yifanen
kaust.authorXü, Yingen
kaust.authorQian, Peiyuanen
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