Involvement of Arabidopsis Hexokinase1 in Cell Death Mediated by Myo -Inositol Accumulation

Handle URI:
http://hdl.handle.net/10754/575652
Title:
Involvement of Arabidopsis Hexokinase1 in Cell Death Mediated by Myo -Inositol Accumulation
Authors:
Bruggeman, Quentin; Prunier, Florence; Mazubert, Christelle; de Bont, Linda; Garmier, Marie; Lugan, Raphaël; Benhamed, Moussa ( 0000-0002-4181-1702 ) ; Bergounioux, Catherine; Raynaud, Cécile; Delarue, Marianne
Abstract:
Programmed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. We recently identified the mips1 mutant of Arabidopsis thaliana, which is deficient for the enzyme catalyzing the limiting step of myo-inositol (MI) synthesis. One of the most striking features of mips1 is the light-dependent formation of lesions on leaves due to salicylic acid (SA)-dependent PCD. Here, we identified a suppressor of PCD by screening for mutations that abolish the mips1 cell death phenotype. Our screen identified the hxk1 mutant, mutated in the gene encoding the hexokinase1 (HXK1) enzyme that catalyzes sugar phosphorylation and acts as a genuine glucose sensor. We show that HXK1 is required for lesion formation in mips1 due to alterations in MI content, via SA-dependant signaling. Using two catalytically inactive HXK1 mutants, we also show that hexokinase catalytic activity is necessary for the establishment of lesions in mips1. Gas chromatography-mass spectrometry analyses revealed a restoration of the MI content in mips1 hxk1 that it is due to the activity of the MIPS2 isoform, while MIPS3 is not involved. Our work defines a pathway of HXK1-mediated cell death in plants and demonstrates that two MIPS enzymes act cooperatively under a particular metabolic status, highlighting a novel checkpoint of MI homeostasis in plants. © 2015 American Society of Plant Biologists. All rights reserved.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture; Bioscience Program; Chromatin and development Research Group
Publisher:
American Society of Plant Biologists (ASPB)
Journal:
The Plant Cell
Issue Date:
5-Jun-2015
DOI:
10.1105/tpc.15.00068
Type:
Article
ISSN:
1040-4651; 1532-298X
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBruggeman, Quentinen
dc.contributor.authorPrunier, Florenceen
dc.contributor.authorMazubert, Christelleen
dc.contributor.authorde Bont, Lindaen
dc.contributor.authorGarmier, Marieen
dc.contributor.authorLugan, Raphaëlen
dc.contributor.authorBenhamed, Moussaen
dc.contributor.authorBergounioux, Catherineen
dc.contributor.authorRaynaud, Cécileen
dc.contributor.authorDelarue, Marianneen
dc.date.accessioned2015-08-24T08:35:04Zen
dc.date.available2015-08-24T08:35:04Zen
dc.date.issued2015-06-05en
dc.identifier.issn1040-4651en
dc.identifier.issn1532-298Xen
dc.identifier.doi10.1105/tpc.15.00068en
dc.identifier.urihttp://hdl.handle.net/10754/575652en
dc.description.abstractProgrammed cell death (PCD) is essential for several aspects of plant life, including development and stress responses. We recently identified the mips1 mutant of Arabidopsis thaliana, which is deficient for the enzyme catalyzing the limiting step of myo-inositol (MI) synthesis. One of the most striking features of mips1 is the light-dependent formation of lesions on leaves due to salicylic acid (SA)-dependent PCD. Here, we identified a suppressor of PCD by screening for mutations that abolish the mips1 cell death phenotype. Our screen identified the hxk1 mutant, mutated in the gene encoding the hexokinase1 (HXK1) enzyme that catalyzes sugar phosphorylation and acts as a genuine glucose sensor. We show that HXK1 is required for lesion formation in mips1 due to alterations in MI content, via SA-dependant signaling. Using two catalytically inactive HXK1 mutants, we also show that hexokinase catalytic activity is necessary for the establishment of lesions in mips1. Gas chromatography-mass spectrometry analyses revealed a restoration of the MI content in mips1 hxk1 that it is due to the activity of the MIPS2 isoform, while MIPS3 is not involved. Our work defines a pathway of HXK1-mediated cell death in plants and demonstrates that two MIPS enzymes act cooperatively under a particular metabolic status, highlighting a novel checkpoint of MI homeostasis in plants. © 2015 American Society of Plant Biologists. All rights reserved.en
dc.publisherAmerican Society of Plant Biologists (ASPB)en
dc.titleInvolvement of Arabidopsis Hexokinase1 in Cell Death Mediated by Myo -Inositol Accumulationen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentChromatin and development Research Groupen
dc.identifier.journalThe Plant Cellen
dc.contributor.institutionUniversité Paris-Sud, Institute of Plant Sciences Paris-Saclay IPS2 (Bâtiment 630), UMR CNRS-INRA 9213, Saclay Plant Sciences, 91405 Orsay, Franceen
dc.contributor.institutionInstitut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 67084 Strasbourg Cedex, Franceen
kaust.authorBenhamed, Moussaen
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