Hepatic Proteomic Responses in Marine Medaka ( Oryzias melastigma ) Chronically Exposed to Antifouling Compound Butenolide [5-octylfuran-2(5H)-one] or 4,5-Dichloro-2- N -Octyl-4-Isothiazolin-3-One (DCOIT)

Handle URI:
http://hdl.handle.net/10754/575639
Title:
Hepatic Proteomic Responses in Marine Medaka ( Oryzias melastigma ) Chronically Exposed to Antifouling Compound Butenolide [5-octylfuran-2(5H)-one] or 4,5-Dichloro-2- N -Octyl-4-Isothiazolin-3-One (DCOIT)
Authors:
Chen, Lianguo; Sun, Jin; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Au, Doris W. T.; Lam, Paul K. S.; Zhang, Weipeng; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Qiu, Jian-Wen; Qian, Pei-Yuan
Abstract:
The pollution of antifoulant SeaNine 211, with 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) as active ingredient, in coastal environment raises concerns on its adverse effects, including endocrine disruption and impairment of reproductive function in marine organisms. In the present study, we investigated the hepatic protein expression profiles of both male and female marine medaka (Oryzias melastigma) exposed to low concentrations of DCOIT at 2.55 mu g/L (0.009 mu M) or butenolide, a promising antifouling agent, at 2.31 mu g/L (0.012 mu M) for 28 days. The results showed that proteins involved in phase I (CYP450 enzyme) metabolism, phase II (UDPGT and GST) conjugation as well as mobilization of retinoid storage, an effective nonenzymatic antioxidant, were consistently up-regulated, possibly facilitating the accelerated detoxification of butenolide. Increased synthesis of bile acid would promote the immediate excretion of butenolide metabolites. Activation of fatty acid beta-oxidation and ATP synthesis were consistent with elevated energy consumption for butenolide degradation and excretion. However, DCOIT did not significantly affect the detoxification system of male medaka, but induced a marked increase of vitellogenin (VTG) by 2.3-fold in the liver of male medaka, suggesting that there is estrogenic activity of DCOIT in endocrine disruption. Overall, this study identified the molecular mechanisms and provided sensitive biomarkers characteristic of butenolide and DCOIT in the liver of marine medaka. The low concentrations of butenolide and DCOIT used in the exposure regimes highlight the needs for systematic evaluation of their environmental risk. In addition, the potent estrogenic activity of DCOIT should be considered in the continued applications of SeaNine 211.
KAUST Department:
Biosciences Core Lab; Core Labs; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC); Applied Mathematics and Computational Science Program
Publisher:
American Chemical Society (ACS)
Journal:
Environmental Science & Technology
Issue Date:
3-Feb-2015
DOI:
10.1021/es5046748
Type:
Article
ISSN:
0013-936X; 1520-5851
Sponsors:
This study was generously supported by a grant (DY125-15-T-02) from China Ocean Mineral Resources Research and Development Association, and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology to P.-Y.Q.
Appears in Collections:
Articles; Biosciences Core Lab; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Lianguoen
dc.contributor.authorSun, Jinen
dc.contributor.authorZhang, Huomingen
dc.contributor.authorAu, Doris W. T.en
dc.contributor.authorLam, Paul K. S.en
dc.contributor.authorZhang, Weipengen
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorQiu, Jian-Wenen
dc.contributor.authorQian, Pei-Yuanen
dc.date.accessioned2015-08-24T08:34:47Zen
dc.date.available2015-08-24T08:34:47Zen
dc.date.issued2015-02-03en
dc.identifier.issn0013-936Xen
dc.identifier.issn1520-5851en
dc.identifier.doi10.1021/es5046748en
dc.identifier.urihttp://hdl.handle.net/10754/575639en
dc.description.abstractThe pollution of antifoulant SeaNine 211, with 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) as active ingredient, in coastal environment raises concerns on its adverse effects, including endocrine disruption and impairment of reproductive function in marine organisms. In the present study, we investigated the hepatic protein expression profiles of both male and female marine medaka (Oryzias melastigma) exposed to low concentrations of DCOIT at 2.55 mu g/L (0.009 mu M) or butenolide, a promising antifouling agent, at 2.31 mu g/L (0.012 mu M) for 28 days. The results showed that proteins involved in phase I (CYP450 enzyme) metabolism, phase II (UDPGT and GST) conjugation as well as mobilization of retinoid storage, an effective nonenzymatic antioxidant, were consistently up-regulated, possibly facilitating the accelerated detoxification of butenolide. Increased synthesis of bile acid would promote the immediate excretion of butenolide metabolites. Activation of fatty acid beta-oxidation and ATP synthesis were consistent with elevated energy consumption for butenolide degradation and excretion. However, DCOIT did not significantly affect the detoxification system of male medaka, but induced a marked increase of vitellogenin (VTG) by 2.3-fold in the liver of male medaka, suggesting that there is estrogenic activity of DCOIT in endocrine disruption. Overall, this study identified the molecular mechanisms and provided sensitive biomarkers characteristic of butenolide and DCOIT in the liver of marine medaka. The low concentrations of butenolide and DCOIT used in the exposure regimes highlight the needs for systematic evaluation of their environmental risk. In addition, the potent estrogenic activity of DCOIT should be considered in the continued applications of SeaNine 211.en
dc.description.sponsorshipThis study was generously supported by a grant (DY125-15-T-02) from China Ocean Mineral Resources Research and Development Association, and an award (SA-C0040/UK-C0016) from the King Abdullah University of Science and Technology to P.-Y.Q.en
dc.publisherAmerican Chemical Society (ACS)en
dc.titleHepatic Proteomic Responses in Marine Medaka ( Oryzias melastigma ) Chronically Exposed to Antifouling Compound Butenolide [5-octylfuran-2(5H)-one] or 4,5-Dichloro-2- N -Octyl-4-Isothiazolin-3-One (DCOIT)en
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentCore Labsen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.identifier.journalEnvironmental Science & Technologyen
dc.contributor.institutionHong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R Chinaen
dc.contributor.institutionHong Kong Univ Sci & Technol, Environm Sci Programs, Hong Kong, Hong Kong, Peoples R Chinaen
dc.contributor.institutionHong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R Chinaen
dc.contributor.institutionCity Univ Hong Kong, Dept Biol & Chem, State Key Lab Marine Pollut, Hong Kong, Hong Kong, Peoples R Chinaen
kaust.authorZhang, Huomingen
kaust.authorBajic, Vladimir B.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.