Diversity and dynamics of dominant and rare bacterial taxa in replicate sequencing batch reactors operated under different solids retention time

Handle URI:
http://hdl.handle.net/10754/575620
Title:
Diversity and dynamics of dominant and rare bacterial taxa in replicate sequencing batch reactors operated under different solids retention time
Authors:
Bagchi, Samik; Garcia Tellez, Berenice; Rao, Hari Ananda ( 0000-0002-8916-1809 ) ; Lamendella, Regina; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
In this study, 16S rRNA gene pyrosequencing was applied in order to provide a better insight on the diversity and dynamics of total, dominant, and rare bacterial taxa in replicate lab-scale sequencing batch reactors (SBRs) operated at different solids retention time (SRT). Rank-abundance curves showed few dominant operational taxonomic units (OTUs) and a long tail of rare OTUs in all reactors. Results revealed that there was no detectable effect of SRT (2 vs. 10 days) on Shannon diversity index and OTU richness of both dominant and rare taxa. Nonmetric multidimensional scaling analysis showed that the total, dominant, and rare bacterial taxa were highly dynamic during the entire period of stable reactor performance. Also, the rare taxa were more dynamic than the dominant taxa despite expected low invasion rates because of the use of sterile synthetic media.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC); Environmental Science and Engineering Program
Publisher:
Springer Science + Business Media
Journal:
Applied Microbiology and Biotechnology
Issue Date:
19-Oct-2014
DOI:
10.1007/s00253-014-6134-4
Type:
Article
ISSN:
01757598
Sponsors:
This research was supported by discretionary investigator funds (P.E.S.) at King Abdullah University of Science and Technology (KAUST). Special thanks are extended to Mohammed Alarawi at the Bioscience Core Laboratory at KAUST for their assistance in 16S rRNA gene pyrosequencing.
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBagchi, Samiken
dc.contributor.authorGarcia Tellez, Bereniceen
dc.contributor.authorRao, Hari Anandaen
dc.contributor.authorLamendella, Reginaen
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2015-08-24T08:34:22Zen
dc.date.available2015-08-24T08:34:22Zen
dc.date.issued2014-10-19en
dc.identifier.issn01757598en
dc.identifier.doi10.1007/s00253-014-6134-4en
dc.identifier.urihttp://hdl.handle.net/10754/575620en
dc.description.abstractIn this study, 16S rRNA gene pyrosequencing was applied in order to provide a better insight on the diversity and dynamics of total, dominant, and rare bacterial taxa in replicate lab-scale sequencing batch reactors (SBRs) operated at different solids retention time (SRT). Rank-abundance curves showed few dominant operational taxonomic units (OTUs) and a long tail of rare OTUs in all reactors. Results revealed that there was no detectable effect of SRT (2 vs. 10 days) on Shannon diversity index and OTU richness of both dominant and rare taxa. Nonmetric multidimensional scaling analysis showed that the total, dominant, and rare bacterial taxa were highly dynamic during the entire period of stable reactor performance. Also, the rare taxa were more dynamic than the dominant taxa despite expected low invasion rates because of the use of sterile synthetic media.en
dc.description.sponsorshipThis research was supported by discretionary investigator funds (P.E.S.) at King Abdullah University of Science and Technology (KAUST). Special thanks are extended to Mohammed Alarawi at the Bioscience Core Laboratory at KAUST for their assistance in 16S rRNA gene pyrosequencing.en
dc.publisherSpringer Science + Business Mediaen
dc.subject16S rRNA gene pyrosequencingen
dc.subjectActivated sludgeen
dc.subjectBacterial community dynamicsen
dc.subjectBacterial diversityen
dc.subjectRare taxaen
dc.titleDiversity and dynamics of dominant and rare bacterial taxa in replicate sequencing batch reactors operated under different solids retention timeen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.identifier.journalApplied Microbiology and Biotechnologyen
dc.contributor.institutionVon Liebig Center for Science, Juniata College, 1700 Moore StreetHuntingdon, PA, United Statesen
kaust.authorBagchi, Samiken
kaust.authorGarcia Tellez, Bereniceen
kaust.authorRao, Hari Anandaen
kaust.authorSaikaly, Pascalen
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