Hierarchical hybrid peroxidase catalysts for remediation of phenol wastewater

Handle URI:
http://hdl.handle.net/10754/575593
Title:
Hierarchical hybrid peroxidase catalysts for remediation of phenol wastewater
Authors:
Duan, Xiaonan; Corgié, Stéphane C.; Aneshansley, Daniel J.; Wang, Peng ( 0000-0003-0856-0865 ) ; Walker, Larry P.; Giannelis, Emmanuel P.
Abstract:
We report a new family of hierarchical hybrid catalysts comprised of horseradish peroxidase (HRP)-magnetic nanoparticles for advanced oxidation processes and demonstrate their utility in the removal of phenol from water. The immobilized HRP catalyzes the oxidation of phenols in the presence of H2O2, producing free radicals. The phenoxy radicals react with each other in a non-enzymatic process to form polymers, which can be removed by precipitation with salts or condensation. The hybrid peroxidase catalysts exhibit three times higher activity than free HRP and are able to remove three times more phenol from water compared to free HRP under similar conditions. In addition, the hybrid catalysts reduce substrate inhibition and limit inactivation from reaction products, which are common problems with free or conventionally immobilized enzymes. Reusability is improved when the HRP-magnetic nanoparticle hybrids are supported on micron-scale magnetic particles, and can be retained with a specially designed magnetically driven reactor. The performance of the hybrid catalysts makes them attractive for several industrial and environmental applications and their development might pave the way for practical applications by eliminating most of the limitations that have prevented the use of free or conventionally immobilized enzymes. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Environmental Nanotechnology Lab
Publisher:
Wiley-Blackwell
Journal:
ChemPhysChem
Issue Date:
20-Feb-2014
DOI:
10.1002/cphc.201300808
PubMed ID:
24692298
Type:
Article
ISSN:
14394235
Sponsors:
This publication was based on work supported by Award No. KUS-C1-018-02, made by the King Abdullah University of Science and Technology (KAUST). We acknowledge use of the Biofuel Research Laboratory (BRL) at Cornell University and the Cornell Center for Materials Research Shared Facilities, which are supported through the National Science Foundation Materials Research Science and Engineering Centers (NSF MRSEC) (DMR-1120296). The magnetically driven reactor part was supported by the US Department of Transportation under contract to the Northeast Sun Grant Initiative at Cornell University #US DOT Assistance #DTOS59-07-G-00052. The authors thank Yan Kang for his help with the SEM images and Dr. Panagiotis Dallas for discussions.
Appears in Collections:
Articles; Environmental Science and Engineering Program; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDuan, Xiaonanen
dc.contributor.authorCorgié, Stéphane C.en
dc.contributor.authorAneshansley, Daniel J.en
dc.contributor.authorWang, Pengen
dc.contributor.authorWalker, Larry P.en
dc.contributor.authorGiannelis, Emmanuel P.en
dc.date.accessioned2015-08-24T08:33:40Zen
dc.date.available2015-08-24T08:33:40Zen
dc.date.issued2014-02-20en
dc.identifier.issn14394235en
dc.identifier.pmid24692298en
dc.identifier.doi10.1002/cphc.201300808en
dc.identifier.urihttp://hdl.handle.net/10754/575593en
dc.description.abstractWe report a new family of hierarchical hybrid catalysts comprised of horseradish peroxidase (HRP)-magnetic nanoparticles for advanced oxidation processes and demonstrate their utility in the removal of phenol from water. The immobilized HRP catalyzes the oxidation of phenols in the presence of H2O2, producing free radicals. The phenoxy radicals react with each other in a non-enzymatic process to form polymers, which can be removed by precipitation with salts or condensation. The hybrid peroxidase catalysts exhibit three times higher activity than free HRP and are able to remove three times more phenol from water compared to free HRP under similar conditions. In addition, the hybrid catalysts reduce substrate inhibition and limit inactivation from reaction products, which are common problems with free or conventionally immobilized enzymes. Reusability is improved when the HRP-magnetic nanoparticle hybrids are supported on micron-scale magnetic particles, and can be retained with a specially designed magnetically driven reactor. The performance of the hybrid catalysts makes them attractive for several industrial and environmental applications and their development might pave the way for practical applications by eliminating most of the limitations that have prevented the use of free or conventionally immobilized enzymes. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en
dc.description.sponsorshipThis publication was based on work supported by Award No. KUS-C1-018-02, made by the King Abdullah University of Science and Technology (KAUST). We acknowledge use of the Biofuel Research Laboratory (BRL) at Cornell University and the Cornell Center for Materials Research Shared Facilities, which are supported through the National Science Foundation Materials Research Science and Engineering Centers (NSF MRSEC) (DMR-1120296). The magnetically driven reactor part was supported by the US Department of Transportation under contract to the Northeast Sun Grant Initiative at Cornell University #US DOT Assistance #DTOS59-07-G-00052. The authors thank Yan Kang for his help with the SEM images and Dr. Panagiotis Dallas for discussions.en
dc.publisherWiley-Blackwellen
dc.subjectEnzyme catalysisen
dc.subjectIron oxideen
dc.subjectNanoparticlesen
dc.subjectPeroxidaseen
dc.subjectPhenol removalen
dc.titleHierarchical hybrid peroxidase catalysts for remediation of phenol wastewateren
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentEnvironmental Nanotechnology Laben
dc.identifier.journalChemPhysChemen
dc.contributor.institutionDepartment of Materials Science and Engineering, Cornell University, Ithaca, NY 14853, United Statesen
dc.contributor.institutionDepartment of Biological and Environmental Engineering, Cornell University, Ithaca, NY 14853, United Statesen
kaust.authorWang, Pengen

Related articles on PubMed

All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.