Exploring neural cell dynamics with digital holographic microscopy

Handle URI:
http://hdl.handle.net/10754/575578
Title:
Exploring neural cell dynamics with digital holographic microscopy
Authors:
Marquet, Pierre; Depeursinge, Christian D.; Magistretti, Pierre J. ( 0000-0002-6678-320X )
Abstract:
In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale particularly suitable for cell imaging. Particular emphasis is placed on the original biological ormation provided by the quantitative phase signal. We present the most relevant DH-QPM applications in the field of cell biology, including automated cell counts, recognition, classification, three-dimensional tracking, discrimination between physiological and pathophysiological states, and the study of cell membrane fluctuations at the nanoscale. In the last part, original results show how DH-QPM can address two important issues in the field of neurobiology, namely, multiple-site optical recording of neuronal activity and noninvasive visualization of dendritic spine dynamics resulting from a full digital holographic microscopy tomographic approach. Copyright © 2013 by Annual Reviews.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program
Publisher:
Annual Reviews
Journal:
Annual Review of Biomedical Engineering
Issue Date:
11-Jul-2013
DOI:
10.1146/annurev-bioeng-071812-152356
PubMed ID:
23662777
Type:
Article
ISSN:
15239829
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMarquet, Pierreen
dc.contributor.authorDepeursinge, Christian D.en
dc.contributor.authorMagistretti, Pierre J.en
dc.date.accessioned2015-08-24T08:33:21Zen
dc.date.available2015-08-24T08:33:21Zen
dc.date.issued2013-07-11en
dc.identifier.issn15239829en
dc.identifier.pmid23662777en
dc.identifier.doi10.1146/annurev-bioeng-071812-152356en
dc.identifier.urihttp://hdl.handle.net/10754/575578en
dc.description.abstractIn this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale particularly suitable for cell imaging. Particular emphasis is placed on the original biological ormation provided by the quantitative phase signal. We present the most relevant DH-QPM applications in the field of cell biology, including automated cell counts, recognition, classification, three-dimensional tracking, discrimination between physiological and pathophysiological states, and the study of cell membrane fluctuations at the nanoscale. In the last part, original results show how DH-QPM can address two important issues in the field of neurobiology, namely, multiple-site optical recording of neuronal activity and noninvasive visualization of dendritic spine dynamics resulting from a full digital holographic microscopy tomographic approach. Copyright © 2013 by Annual Reviews.en
dc.publisherAnnual Reviewsen
dc.subjectcell imagingen
dc.subjectdendritic spinesen
dc.subjectdiffraction tomographyen
dc.subjectdigital holographic microscopyen
dc.subjectneuronal activitiesen
dc.subjectquantitative phase microscopyen
dc.titleExploring neural cell dynamics with digital holographic microscopyen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.identifier.journalAnnual Review of Biomedical Engineeringen
dc.contributor.institutionCentre de Neurosciences Psychiatriques, Centre Hospitalier Universitaire Vaudois (CHUV), Département de Psychiatrie, Site de Cery, CH-1008 Prilly/Lausanne, Switzerlanden
dc.contributor.institutionBrain Mind Institute, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerlanden
dc.contributor.institutionInstitute of Microengineering, École Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerlanden
kaust.authorMagistretti, Pierre J.en

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