Multiple and high-throughput droplet reactions via combination of microsampling technique and microfluidic chip

Handle URI:
http://hdl.handle.net/10754/575567
Title:
Multiple and high-throughput droplet reactions via combination of microsampling technique and microfluidic chip
Authors:
Wu, Jinbo; Zhang, Mengying; Li, Xiaolin; Wen, Weijia
Abstract:
Microdroplets offer unique compartments for accommodating a large number of chemical and biological reactions in tiny volume with precise control. A major concern in droplet-based microfluidics is the difficulty to address droplets individually and achieve high throughput at the same time. Here, we have combined an improved cartridge sampling technique with a microfluidic chip to perform droplet screenings and aggressive reaction with minimal (nanoliter-scale) reagent consumption. The droplet composition, distance, volume (nanoliter to subnanoliter scale), number, and sequence could be precisely and digitally programmed through the improved sampling technique, while sample evaporation and cross-contamination are effectively eliminated. Our combined device provides a simple model to utilize multiple droplets for various reactions with low reagent consumption and high throughput. © 2012 American Chemical Society.
KAUST Department:
KAUST-HKUST Micro/Nanofluidic Joint Laboratory; Environmental Science and Engineering Program
Publisher:
American Chemical Society (ACS)
Journal:
Analytical Chemistry
Issue Date:
20-Nov-2012
DOI:
10.1021/ac302249h
Type:
Article
ISSN:
00032700
Sponsors:
This publication is based on work partially supported by Award No. SA-C0040/UK-C0016, made by King Abdullah University of Science and Technology (KAUST), Hong Kong RGC grants HKUST 604710 and 605411.
Appears in Collections:
Articles; Environmental Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorWu, Jinboen
dc.contributor.authorZhang, Mengyingen
dc.contributor.authorLi, Xiaolinen
dc.contributor.authorWen, Weijiaen
dc.date.accessioned2015-08-24T08:33:04Zen
dc.date.available2015-08-24T08:33:04Zen
dc.date.issued2012-11-20en
dc.identifier.issn00032700en
dc.identifier.doi10.1021/ac302249hen
dc.identifier.urihttp://hdl.handle.net/10754/575567en
dc.description.abstractMicrodroplets offer unique compartments for accommodating a large number of chemical and biological reactions in tiny volume with precise control. A major concern in droplet-based microfluidics is the difficulty to address droplets individually and achieve high throughput at the same time. Here, we have combined an improved cartridge sampling technique with a microfluidic chip to perform droplet screenings and aggressive reaction with minimal (nanoliter-scale) reagent consumption. The droplet composition, distance, volume (nanoliter to subnanoliter scale), number, and sequence could be precisely and digitally programmed through the improved sampling technique, while sample evaporation and cross-contamination are effectively eliminated. Our combined device provides a simple model to utilize multiple droplets for various reactions with low reagent consumption and high throughput. © 2012 American Chemical Society.en
dc.description.sponsorshipThis publication is based on work partially supported by Award No. SA-C0040/UK-C0016, made by King Abdullah University of Science and Technology (KAUST), Hong Kong RGC grants HKUST 604710 and 605411.en
dc.publisherAmerican Chemical Society (ACS)en
dc.titleMultiple and high-throughput droplet reactions via combination of microsampling technique and microfluidic chipen
dc.typeArticleen
dc.contributor.departmentKAUST-HKUST Micro/Nanofluidic Joint Laboratoryen
dc.contributor.departmentEnvironmental Science and Engineering Programen
dc.identifier.journalAnalytical Chemistryen
kaust.authorWu, Jinboen
kaust.authorZhang, Mengyingen
kaust.authorLi, Xiaolinen
kaust.authorWen, Weijiaen
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