Marinobacter xestospongiae sp. nov., isolated from the marine sponge Xestospongia testudinaria collected from the Red Sea

Handle URI:
http://hdl.handle.net/10754/575553
Title:
Marinobacter xestospongiae sp. nov., isolated from the marine sponge Xestospongia testudinaria collected from the Red Sea
Authors:
Lee, O. O.; Lai, P. Y.; Wu, H.-x.; Zhou, X.-j.; Miao, L.; Wang, H.; Qian, P.-Y.
Abstract:
A Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped and slightly halophilic bacterial strain, designated UST090418-1611(T), was isolated from the marina sponge Xestospongia testudinaria collected from the Red Sea coast of Saudi Arabia. Phylogenetic trees based on the 16S rRNA gene sequence placed strain UST090418-1611(T) in the family Alteromonadaceae with the closest relationship to the genus Marinobacter. The 16S rRNA gene sequence similarity between the strain and the type strains of recognized Marinobacter species ranged from 92.9 to 98.3%. Although strain UST090418-1611(T) shared high 16S rRNA gene sequence similarity with Marinobacter mobilis CN46(T), M. zhejiangensis CN74(T) and M. sediminum R65(T) (98.3, 97.4 and 97.3%, respectively), the relatedness of the strain to these three strains in DNA DNA hybridization was only 58, 56 and 33%, respectively, supporting the novelty of the strain. In contrast to most strains in the genus Marinobacter, strain UST090418-1611(T) tolerated only 6% (w/v) NaCl, and optimal growth occurred at 2.0% (w/v) NaCl, pH 7.0-8.0 and 28-36 degrees C. The predominant cellular fatty acids were C-12:0 3-OH, C-16:0, C-12:0 and summed feature 3 (C-16.1 omega 6c and/or C-16:1 omega 7c) The genomic DNA G+C content was 57.1 mol%. Based on the physiological, phylogenetic and chemotaxonomic characteristics presented in this study, we suggest that the strain represents a novel species in the genus Marinobacter, for which the name Marinobacter xestospongiae sp. nov. is proposed, with UST090418-1611(T) (=JCM 17469(T) =NRRL B-59512(T)) as the type strain.
KAUST Department:
Materials Science and Engineering Program
Publisher:
Microbiology Society
Journal:
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Issue Date:
14-Oct-2011
DOI:
10.1099/ijs.0.028811-0
Type:
Article
ISSN:
1466-5026; 1466-5034
Sponsors:
The authors thank H. C. Chung, Y. H. Wong, M. Li and J. P. Ren from the Hong Kong University of Science and Technology (HKUST), D. Lau from the Baptist University of Hong Kong and the technical team from King Abdullah University of Science and Technology (KAUST) for technical help during sample collection. We also thank Professor Rob von Soest, Zoological Museum, University of Amsterdam, for identification of sponges, and Dr J. P. Euzeby for advice on nomenclature. This study was supported by an award made by KAUST (SA-C0040/UK-C0016) to P.-Y. Q.
Appears in Collections:
Articles; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorLee, O. O.en
dc.contributor.authorLai, P. Y.en
dc.contributor.authorWu, H.-x.en
dc.contributor.authorZhou, X.-j.en
dc.contributor.authorMiao, L.en
dc.contributor.authorWang, H.en
dc.contributor.authorQian, P.-Y.en
dc.date.accessioned2015-08-24T08:32:41Zen
dc.date.available2015-08-24T08:32:41Zen
dc.date.issued2011-10-14en
dc.identifier.issn1466-5026en
dc.identifier.issn1466-5034en
dc.identifier.doi10.1099/ijs.0.028811-0en
dc.identifier.urihttp://hdl.handle.net/10754/575553en
dc.description.abstractA Gram-negative, catalase- and oxidase-positive, non-sporulating, rod-shaped and slightly halophilic bacterial strain, designated UST090418-1611(T), was isolated from the marina sponge Xestospongia testudinaria collected from the Red Sea coast of Saudi Arabia. Phylogenetic trees based on the 16S rRNA gene sequence placed strain UST090418-1611(T) in the family Alteromonadaceae with the closest relationship to the genus Marinobacter. The 16S rRNA gene sequence similarity between the strain and the type strains of recognized Marinobacter species ranged from 92.9 to 98.3%. Although strain UST090418-1611(T) shared high 16S rRNA gene sequence similarity with Marinobacter mobilis CN46(T), M. zhejiangensis CN74(T) and M. sediminum R65(T) (98.3, 97.4 and 97.3%, respectively), the relatedness of the strain to these three strains in DNA DNA hybridization was only 58, 56 and 33%, respectively, supporting the novelty of the strain. In contrast to most strains in the genus Marinobacter, strain UST090418-1611(T) tolerated only 6% (w/v) NaCl, and optimal growth occurred at 2.0% (w/v) NaCl, pH 7.0-8.0 and 28-36 degrees C. The predominant cellular fatty acids were C-12:0 3-OH, C-16:0, C-12:0 and summed feature 3 (C-16.1 omega 6c and/or C-16:1 omega 7c) The genomic DNA G+C content was 57.1 mol%. Based on the physiological, phylogenetic and chemotaxonomic characteristics presented in this study, we suggest that the strain represents a novel species in the genus Marinobacter, for which the name Marinobacter xestospongiae sp. nov. is proposed, with UST090418-1611(T) (=JCM 17469(T) =NRRL B-59512(T)) as the type strain.en
dc.description.sponsorshipThe authors thank H. C. Chung, Y. H. Wong, M. Li and J. P. Ren from the Hong Kong University of Science and Technology (HKUST), D. Lau from the Baptist University of Hong Kong and the technical team from King Abdullah University of Science and Technology (KAUST) for technical help during sample collection. We also thank Professor Rob von Soest, Zoological Museum, University of Amsterdam, for identification of sponges, and Dr J. P. Euzeby for advice on nomenclature. This study was supported by an award made by KAUST (SA-C0040/UK-C0016) to P.-Y. Q.en
dc.publisherMicrobiology Societyen
dc.titleMarinobacter xestospongiae sp. nov., isolated from the marine sponge Xestospongia testudinaria collected from the Red Seaen
dc.typeArticleen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalINTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGYen
dc.contributor.institutionShanghai Ocean Univ, Coll Fisheries & Life Sci, Lingang New City, Shanghai, Peoples R Chinaen
dc.contributor.institutionYangzhou Univ, Inst Marine Sci & Technol, Yangzhou City, Jiangsu, Peoples R Chinaen
kaust.authorWang, Haoen
kaust.authorLee, On Onen
kaust.authorLai, Pok Yuien
kaust.authorWu, Hui-xianen
kaust.authorQian, Pei-Yuanen
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