Study of monocyte membrane proteome perturbation during lipopolysaccharide-induced tolerance using iTRAQ-based quantitative proteomic approach

Handle URI:
http://hdl.handle.net/10754/575541
Title:
Study of monocyte membrane proteome perturbation during lipopolysaccharide-induced tolerance using iTRAQ-based quantitative proteomic approach
Authors:
Zhang, Huoming ( 0000-0001-5416-0358 ) ; Zhao, Changqing; Li, Xin; Zhu, Yi; Gan, Chee Sian; Wang, Yong; Ravasi, Timothy ( 0000-0002-9950-465X ) ; Qian, Pei-Yuan; Wong, Siew Cheng; Sze, Siu Kwan
Abstract:
Human monocytes' exposure to low-level lipopolysaccharide (LPS) induces temporary monocytic insensitivity to subsequent LPS challenge. The underlying mechanism of this phenomenon could have important clinical utilities in preventing and/or treating severe infections. In this study, we used an iTRAQ-based quantitative proteomic approach to comprehensively characterize the membrane proteomes of monocytes before and after LPS exposure. We identified a total of 1651 proteins, of which 53.6% were membrane proteins. Ninety-four percent of the proteins were quantified and 255 proteins were shown to be tightly regulated by LPS. Subcellular location analysis revealed organelle-specific response to LPS exposure: more than 90% of identified mitochondrial membrane proteins were significant downregulated, whereas the majority of proteins from other organelles such as ER, Golgi and ribosome were upregulated. Moreover, we found that the expression of most receptors potentially involved in LPS signal pathway (CD14, toll-like receptor 4, CD11/CD18 complex) were substantially decreased, while the expression of molecules involved in LPS neutralization were enhanced after LPS challenge. Together, these findings could be of significance in understanding the mechanism of LPS tolerance and provide values for designing new approaches for regulating monocytic responses in sepsis patients.
KAUST Department:
Biosciences Core Lab; Core Labs; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Computational Bioscience Research Center (CBRC); Bioscience Program; Integrative Systems Biology Lab
Publisher:
Wiley-Blackwell
Journal:
PROTEOMICS
Issue Date:
2-Jul-2010
DOI:
10.1002/pmic.201000066
Type:
Article
ISSN:
1615-9853
Sponsors:
This work was supported by grants from the Biomedical Research Council (BMRC: 07/1/22/19/531) and the Ministry of Education (ARC: T206B3211) of Singapore.
Appears in Collections:
Articles; Bioscience Program; Biosciences Core Lab; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZhang, Huomingen
dc.contributor.authorZhao, Changqingen
dc.contributor.authorLi, Xinen
dc.contributor.authorZhu, Yien
dc.contributor.authorGan, Chee Sianen
dc.contributor.authorWang, Yongen
dc.contributor.authorRavasi, Timothyen
dc.contributor.authorQian, Pei-Yuanen
dc.contributor.authorWong, Siew Chengen
dc.contributor.authorSze, Siu Kwanen
dc.date.accessioned2015-08-24T08:32:21Zen
dc.date.available2015-08-24T08:32:21Zen
dc.date.issued2010-07-02en
dc.identifier.issn1615-9853en
dc.identifier.doi10.1002/pmic.201000066en
dc.identifier.urihttp://hdl.handle.net/10754/575541en
dc.description.abstractHuman monocytes' exposure to low-level lipopolysaccharide (LPS) induces temporary monocytic insensitivity to subsequent LPS challenge. The underlying mechanism of this phenomenon could have important clinical utilities in preventing and/or treating severe infections. In this study, we used an iTRAQ-based quantitative proteomic approach to comprehensively characterize the membrane proteomes of monocytes before and after LPS exposure. We identified a total of 1651 proteins, of which 53.6% were membrane proteins. Ninety-four percent of the proteins were quantified and 255 proteins were shown to be tightly regulated by LPS. Subcellular location analysis revealed organelle-specific response to LPS exposure: more than 90% of identified mitochondrial membrane proteins were significant downregulated, whereas the majority of proteins from other organelles such as ER, Golgi and ribosome were upregulated. Moreover, we found that the expression of most receptors potentially involved in LPS signal pathway (CD14, toll-like receptor 4, CD11/CD18 complex) were substantially decreased, while the expression of molecules involved in LPS neutralization were enhanced after LPS challenge. Together, these findings could be of significance in understanding the mechanism of LPS tolerance and provide values for designing new approaches for regulating monocytic responses in sepsis patients.en
dc.description.sponsorshipThis work was supported by grants from the Biomedical Research Council (BMRC: 07/1/22/19/531) and the Ministry of Education (ARC: T206B3211) of Singapore.en
dc.publisherWiley-Blackwellen
dc.titleStudy of monocyte membrane proteome perturbation during lipopolysaccharide-induced tolerance using iTRAQ-based quantitative proteomic approachen
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentCore Labsen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentBioscience Programen
dc.contributor.departmentIntegrative Systems Biology Laben
dc.identifier.journalPROTEOMICSen
dc.contributor.institutionNanyang Technol Univ, Sch Biol Sci, Singapore, Singaporeen
dc.contributor.institutionHong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R Chinaen
dc.contributor.institutionAgcy Sci Technol & Res, Singapore Immunol Network, Biopolis, Singaporeen
kaust.authorZhang, Huomingen
kaust.authorWang, Yongen
kaust.authorRavasi, Timothyen
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.