Involvement of a novel p38 mitogen-activated protein kinase in larval metamorphosis of the polychaete Hydroides elegans (Haswell)

Handle URI:
http://hdl.handle.net/10754/575540
Title:
Involvement of a novel p38 mitogen-activated protein kinase in larval metamorphosis of the polychaete Hydroides elegans (Haswell)
Authors:
Wang, Hao; Qian, Peiyuan
Abstract:
Hydroides elegans is a common marine fouling organism in most tropical and subtropical waters. The life cycle of H. elegans includes a planktonic larval stage in which swimming larvae normally take 5 days to attain competency to settle. Larval metamorphosis marks the beginning of its benthic life; however, the endogenous molecular mechanisms that regulate metamorphosis remain largely unknown. In this study, a PCR-based suppressive subtractive hybridization (SSH) library was constructed to screen the genes expressed in competent larvae but not in precompetent larvae. Among the transcripts isolated from the library, 21 significantly matched sequences in the GenBank. Many of these isolated transcripts have putative roles in the reactive oxygen species (ROS) signal transduction pathway or in response to ROS stress. A putative novel p38 mitogen-activated protein kinase (MAPK), which was also isolated with SSH screen, was then cloned and characterized. The MAPK inhibitors assay showed that both p38 MAPK inhibitors SB202190 and SB203580 effectively inhibited the biofilm-induced metamorphosis of H. elegans. A cell stressors assay showed that H2O2 effectively induced larval metamorphosis of H. elegans, but the inductivity of H2O2 was also inhibited by both SB inhibitors. The catalase assay showed that the catalase could effetely inhibit H. elegans larvae from responding to inductive biofilm. These results showed that the p38 MAPK-dependent pathway plays critical role in controlling larval metamorphosis of the marine polychaete H. elegans, and the reactive oxygen radicals produced by biofilm could be the cue inducing larval metamorphosis. © 2010 Wiley-Liss, Inc.
KAUST Department:
KAUST Global Collaborative Research Program; Materials Science and Engineering Program
Publisher:
Wiley-Blackwell
Journal:
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
Issue Date:
19-Apr-2010
DOI:
10.1002/jez.b.21344
PubMed ID:
20535771
Type:
Article
ISSN:
15525007
Sponsors:
Grant Sponsor: The Hong Kong Government; Grant number: HKUST6418/06M; Grant Sponsor: King Abdullah University of Science and Technology; Grant number: SA-C0040/UK-00016.
Appears in Collections:
Articles; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Haoen
dc.contributor.authorQian, Peiyuanen
dc.date.accessioned2015-08-24T08:32:19Zen
dc.date.available2015-08-24T08:32:19Zen
dc.date.issued2010-04-19en
dc.identifier.issn15525007en
dc.identifier.pmid20535771en
dc.identifier.doi10.1002/jez.b.21344en
dc.identifier.urihttp://hdl.handle.net/10754/575540en
dc.description.abstractHydroides elegans is a common marine fouling organism in most tropical and subtropical waters. The life cycle of H. elegans includes a planktonic larval stage in which swimming larvae normally take 5 days to attain competency to settle. Larval metamorphosis marks the beginning of its benthic life; however, the endogenous molecular mechanisms that regulate metamorphosis remain largely unknown. In this study, a PCR-based suppressive subtractive hybridization (SSH) library was constructed to screen the genes expressed in competent larvae but not in precompetent larvae. Among the transcripts isolated from the library, 21 significantly matched sequences in the GenBank. Many of these isolated transcripts have putative roles in the reactive oxygen species (ROS) signal transduction pathway or in response to ROS stress. A putative novel p38 mitogen-activated protein kinase (MAPK), which was also isolated with SSH screen, was then cloned and characterized. The MAPK inhibitors assay showed that both p38 MAPK inhibitors SB202190 and SB203580 effectively inhibited the biofilm-induced metamorphosis of H. elegans. A cell stressors assay showed that H2O2 effectively induced larval metamorphosis of H. elegans, but the inductivity of H2O2 was also inhibited by both SB inhibitors. The catalase assay showed that the catalase could effetely inhibit H. elegans larvae from responding to inductive biofilm. These results showed that the p38 MAPK-dependent pathway plays critical role in controlling larval metamorphosis of the marine polychaete H. elegans, and the reactive oxygen radicals produced by biofilm could be the cue inducing larval metamorphosis. © 2010 Wiley-Liss, Inc.en
dc.description.sponsorshipGrant Sponsor: The Hong Kong Government; Grant number: HKUST6418/06M; Grant Sponsor: King Abdullah University of Science and Technology; Grant number: SA-C0040/UK-00016.en
dc.publisherWiley-Blackwellen
dc.titleInvolvement of a novel p38 mitogen-activated protein kinase in larval metamorphosis of the polychaete Hydroides elegans (Haswell)en
dc.typeArticleen
dc.contributor.departmentKAUST Global Collaborative Research Programen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalJournal of Experimental Zoology Part B: Molecular and Developmental Evolutionen
kaust.authorWang, Haoen
kaust.authorQian, Peiyuanen
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