The potato carotenoid cleavage dioxygenase 4 catalyzes a single cleavage of β-ionone ring-containing carotenes and non-epoxidated xanthophylls

Handle URI:
http://hdl.handle.net/10754/566179
Title:
The potato carotenoid cleavage dioxygenase 4 catalyzes a single cleavage of β-ionone ring-containing carotenes and non-epoxidated xanthophylls
Authors:
Bruno, Mark; Beyer, Peter D.; Al-Babili, Salim ( 0000-0003-4823-2882 )
Abstract:
Down-regulation of the potato carotenoid cleavage dioxygenase 4 (StCCD4) transcript level led to tubers with altered morphology and sprouting activity, which also accumulated higher levels of violaxanthin and lutein leading to elevated carotenoid amounts. This phenotype indicates a role of this enzyme in tuber development, which may be exerted by a cleavage product. In this work, we investigated the enzymatic activity of StCCD4, by expressing the corresponding cDNA in carotenoid accumulating Escherichia coli strains and by performing in vitro assays with heterologously expressed enzyme. StCCD4 catalyzed the cleavage of all-. trans-β-carotene at the C9'-C10' double bond, leading to β-ionone and all-. trans-β-apo-10'-carotenal, both in vivo and in vitro. The enzyme also cleaved β,β-cryptoxanthin, zeaxanthin and lutein either at the C9'-C10' or the C9-C10 double bond in vitro. In contrast, we did not observe any conversion of violaxanthin and only traces of activity with 9-. cis-β-carotene, which led to 9-. cis-β-apo-10'-carotenal. Our data indicate that all-. trans-β-carotene is the likely substrate of StCCD4 in planta, and that this carotene may be precursor of an unknown compound involved in tuber development.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Center for Desert Agriculture
Publisher:
Elsevier BV
Journal:
Archives of Biochemistry and Biophysics
Issue Date:
Apr-2015
DOI:
10.1016/j.abb.2015.02.011
Type:
Article
ISSN:
00039861
Sponsors:
We thank Dr. Hansgeorg Ernst for providing the synthetic substrates and Erdmann Scheffer for skillful technical assistance. This work was supported by the European Union (METAPRO; FP7 KBBE-2009-3-1-01) and by the King Abdullah University of Science and Technology (KAUST).
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorBruno, Marken
dc.contributor.authorBeyer, Peter D.en
dc.contributor.authorAl-Babili, Salimen
dc.date.accessioned2015-08-12T09:31:20Zen
dc.date.available2015-08-12T09:31:20Zen
dc.date.issued2015-04en
dc.identifier.issn00039861en
dc.identifier.doi10.1016/j.abb.2015.02.011en
dc.identifier.urihttp://hdl.handle.net/10754/566179en
dc.description.abstractDown-regulation of the potato carotenoid cleavage dioxygenase 4 (StCCD4) transcript level led to tubers with altered morphology and sprouting activity, which also accumulated higher levels of violaxanthin and lutein leading to elevated carotenoid amounts. This phenotype indicates a role of this enzyme in tuber development, which may be exerted by a cleavage product. In this work, we investigated the enzymatic activity of StCCD4, by expressing the corresponding cDNA in carotenoid accumulating Escherichia coli strains and by performing in vitro assays with heterologously expressed enzyme. StCCD4 catalyzed the cleavage of all-. trans-β-carotene at the C9'-C10' double bond, leading to β-ionone and all-. trans-β-apo-10'-carotenal, both in vivo and in vitro. The enzyme also cleaved β,β-cryptoxanthin, zeaxanthin and lutein either at the C9'-C10' or the C9-C10 double bond in vitro. In contrast, we did not observe any conversion of violaxanthin and only traces of activity with 9-. cis-β-carotene, which led to 9-. cis-β-apo-10'-carotenal. Our data indicate that all-. trans-β-carotene is the likely substrate of StCCD4 in planta, and that this carotene may be precursor of an unknown compound involved in tuber development.en
dc.description.sponsorshipWe thank Dr. Hansgeorg Ernst for providing the synthetic substrates and Erdmann Scheffer for skillful technical assistance. This work was supported by the European Union (METAPRO; FP7 KBBE-2009-3-1-01) and by the King Abdullah University of Science and Technology (KAUST).en
dc.publisherElsevier BVen
dc.subjectApocarotenoidsen
dc.subjectCarotenoid cleavage dioxygenasesen
dc.subjectCarotenoidsen
dc.subjectPotatoen
dc.titleThe potato carotenoid cleavage dioxygenase 4 catalyzes a single cleavage of β-ionone ring-containing carotenes and non-epoxidated xanthophyllsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalArchives of Biochemistry and Biophysicsen
dc.contributor.institutionAlbert-Ludwigs University of Freiburg, Faculty of Biology, Schaenzlestr. 1, D-79104 Freiburg, Germanyen
kaust.authorAl-Babili, Salimen
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