Surface enhanced Raman spectroscopy measurements of MCF7 cells adhesion in confined micro-environments

Handle URI:
http://hdl.handle.net/10754/566172
Title:
Surface enhanced Raman spectroscopy measurements of MCF7 cells adhesion in confined micro-environments
Authors:
De Vitis, Stefania; Coluccio, Maria Laura; Gentile, Francesco; Malara, Natalia; Perozziello, Gerardo; Dattola, Elisabetta; Candeloro, Patrizio; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
Undoubtedly cells can perceive the external environment, not only from a biochemical point of view with the related signalling pathways, but also from a physical and topographical perspective. In this sense controlled three dimensional micro-structures as well as patterns at the nano-scale can affect and guide the cell evolution and proliferation, due to the fact that the surrounding environment is no longer isotropic (like the flat surfaces of standard cell culturing) but possesses well defined symmetries and anisotropies. In this work regular arrays of silicon micro-pillars with hexagonal arrangement are used as culturing substrates for MCF-7 breast cancer cells. The characteristic size and spacing of the pillars are tens of microns, comparable with MCF-7 cell dimensions and then well suited to induce acceptable external stimuli. It is shown that these cells strongly modify their morphology for adapting themselves to the micro-structured landscape, by means of protrusions from the main body of the cell. Scanning electron microscopy along with both Raman micro-spectroscopy and surface enhanced Raman spectroscopy are used for topographical and biochemical studies of the new cell arrangement. We have revealed that single MCF-7 cells exploit their capability to produce invadopodia, usually generated to invade the neighboring tissue in metastatic activity, for spanning and growing across separate pillars. © 2015 Elsevier Ltd.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Physical Sciences and Engineering (PSE) Division
Publisher:
Elsevier BV
Journal:
Optics and Lasers in Engineering
Issue Date:
May-2015
DOI:
10.1016/j.optlaseng.2015.04.010
Type:
Article
ISSN:
01438166
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDe Vitis, Stefaniaen
dc.contributor.authorColuccio, Maria Lauraen
dc.contributor.authorGentile, Francescoen
dc.contributor.authorMalara, Nataliaen
dc.contributor.authorPerozziello, Gerardoen
dc.contributor.authorDattola, Elisabettaen
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-08-12T09:31:02Zen
dc.date.available2015-08-12T09:31:02Zen
dc.date.issued2015-05en
dc.identifier.issn01438166en
dc.identifier.doi10.1016/j.optlaseng.2015.04.010en
dc.identifier.urihttp://hdl.handle.net/10754/566172en
dc.description.abstractUndoubtedly cells can perceive the external environment, not only from a biochemical point of view with the related signalling pathways, but also from a physical and topographical perspective. In this sense controlled three dimensional micro-structures as well as patterns at the nano-scale can affect and guide the cell evolution and proliferation, due to the fact that the surrounding environment is no longer isotropic (like the flat surfaces of standard cell culturing) but possesses well defined symmetries and anisotropies. In this work regular arrays of silicon micro-pillars with hexagonal arrangement are used as culturing substrates for MCF-7 breast cancer cells. The characteristic size and spacing of the pillars are tens of microns, comparable with MCF-7 cell dimensions and then well suited to induce acceptable external stimuli. It is shown that these cells strongly modify their morphology for adapting themselves to the micro-structured landscape, by means of protrusions from the main body of the cell. Scanning electron microscopy along with both Raman micro-spectroscopy and surface enhanced Raman spectroscopy are used for topographical and biochemical studies of the new cell arrangement. We have revealed that single MCF-7 cells exploit their capability to produce invadopodia, usually generated to invade the neighboring tissue in metastatic activity, for spanning and growing across separate pillars. © 2015 Elsevier Ltd.en
dc.publisherElsevier BVen
dc.subjectInvadopodiaen
dc.subjectMCF-7 breast cancer cellen
dc.subjectRaman micro-spectroscopyen
dc.subjectSuperhydrophobic surfaceen
dc.subjectSurface enhanced Raman spectroscopy (SERS)en
dc.titleSurface enhanced Raman spectroscopy measurements of MCF7 cells adhesion in confined micro-environmentsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalOptics and Lasers in Engineeringen
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University Magna Graecia of Catanzaro, 88100 Catanzaro, Italyen
dc.contributor.institutionDepartment of Electrical Engineering and Information Technology, University of Naples, 80125 Naples, Italyen
dc.contributor.institutionCellular Toxicological Laboratory, Department of Health Science, University Magna Graecia of Catanzaro, 88100 Catanzaro, Italyen
kaust.authorDi Fabrizio, Enzo M.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.