Numerical model for the deformation of nucleated cells by optical stretchers

Handle URI:
http://hdl.handle.net/10754/566140
Title:
Numerical model for the deformation of nucleated cells by optical stretchers
Authors:
Sraj, Ihab ( 0000-0002-6158-472X ) ; Francois, Joshua; Marr, David W M; Eggleton, Charles D.
Abstract:
In this paper, we seek to numerically study the deformation of nucleated cells by single diode-laser bar optical stretchers. We employ a recently developed computational model, the dynamic ray-tracing method, to determine the force distribution induced by optical stretchers on a cell encapsulating a nucleus of different optical properties. These optical forces are shape dependent and can deform real non-rigid objects; thus resulting in dynamically changing distributions with cell and nucleus deformation. A Chinese hamster ovary (CHO) cell is a common biological cell that is of interest to the biomedical community because of its use in recombinant protein therapeutics and is an example of a nucleated cell. To this end, we model CHO cells as two concentric three-dimensional elastic capsules immersed in a fluid where the hydrodynamic forces are calculated using the immersed boundary method. We vary the inner capsule size to simulate different nucleus sizes. Our results show that the presence of a nucleus has a major effect on the force distribution on the cell surface and consequently on its net deformation. Scattering and gradient forces are reported for different nucleus sizes and the effect of nucleus size on the cell deformation is discussed quantitatively. © 2015 IOP Publishing Ltd.
KAUST Department:
Chemical and Biological Engineering Program; Mechanical Engineering Program; Physical Sciences and Engineering (PSE) Division; Earth Science and Engineering Program
Publisher:
IOP Publishing
Journal:
Journal of Optics
Issue Date:
1-Jul-2015
DOI:
10.1088/2040-8978/17/7/075403
Type:
Article
ISSN:
2040-8978; 2040-8986
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Chemical and Biological Engineering Program; Earth Science and Engineering Program; Mechanical Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorSraj, Ihaben
dc.contributor.authorFrancois, Joshuaen
dc.contributor.authorMarr, David W Men
dc.contributor.authorEggleton, Charles D.en
dc.date.accessioned2015-08-12T09:29:50Zen
dc.date.available2015-08-12T09:29:50Zen
dc.date.issued2015-07-01en
dc.identifier.issn2040-8978en
dc.identifier.issn2040-8986en
dc.identifier.doi10.1088/2040-8978/17/7/075403en
dc.identifier.urihttp://hdl.handle.net/10754/566140en
dc.description.abstractIn this paper, we seek to numerically study the deformation of nucleated cells by single diode-laser bar optical stretchers. We employ a recently developed computational model, the dynamic ray-tracing method, to determine the force distribution induced by optical stretchers on a cell encapsulating a nucleus of different optical properties. These optical forces are shape dependent and can deform real non-rigid objects; thus resulting in dynamically changing distributions with cell and nucleus deformation. A Chinese hamster ovary (CHO) cell is a common biological cell that is of interest to the biomedical community because of its use in recombinant protein therapeutics and is an example of a nucleated cell. To this end, we model CHO cells as two concentric three-dimensional elastic capsules immersed in a fluid where the hydrodynamic forces are calculated using the immersed boundary method. We vary the inner capsule size to simulate different nucleus sizes. Our results show that the presence of a nucleus has a major effect on the force distribution on the cell surface and consequently on its net deformation. Scattering and gradient forces are reported for different nucleus sizes and the effect of nucleus size on the cell deformation is discussed quantitatively. © 2015 IOP Publishing Ltd.en
dc.publisherIOP Publishingen
dc.subjectcell deformationen
dc.subjectdynamic ray tracingen
dc.subjectoptical trappingen
dc.titleNumerical model for the deformation of nucleated cells by optical stretchersen
dc.typeArticleen
dc.contributor.departmentChemical and Biological Engineering Programen
dc.contributor.departmentMechanical Engineering Programen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentEarth Science and Engineering Programen
dc.identifier.journalJournal of Opticsen
kaust.authorSraj, Ihaben
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.