Membrane-anchored MucR mediates nitrate-dependent regulation of alginate production in Pseudomonas aeruginosa

Handle URI:
http://hdl.handle.net/10754/566131
Title:
Membrane-anchored MucR mediates nitrate-dependent regulation of alginate production in Pseudomonas aeruginosa
Authors:
Wang, Yajie; Hay, Iain D.; Rehman, Zahid Ur ( 0000-0001-6904-6435 ) ; Rehm, Bernd H A
Abstract:
Alginates exhibit unique material properties suitable for medical and industrial applications. However, if produced by Pseudomonas aeruginosa, it is an important virulence factor in infection of cystic fibrosis patients. The alginate biosynthesis machinery is activated by c-di-GMP imparted by the inner membrane protein, MucR. Here, it was shown that MucR impairs alginate production in response to nitrate in P. aeruginosa. Subsequent site-specific mutagenesis of MucR revealed that the second MHYT sensor motif (MHYT II, amino acids 121–124) of MucR sensor domain was involved in nitrate sensing. We also showed that both c-di-GMP synthesizing and degrading active sites of MucR were important for alginate production. Although nitrate and deletion of MucR impaired alginate promoter activity and global c-di-GMP levels, alginate yields were not directly correlated with alginate promoter activity or c-di-GMP levels, suggesting that nitrate and MucR modulate alginate production at a post-translational level through a localized pool of c-di-GMP. Nitrate increased pel promoter activity in the mucR mutant while in the same mutant the psl promoter activity was independent of nitrate. Nitrate and deletion of mucR did not impact on swarming motility but impaired attachment to solid surfaces. Nitrate and deletion of mucR promoted the formation of biofilms with increased thickness, cell density, and survival. Overall, this study provided insight into the functional role of MucR with respect to nitrate-mediated regulation of alginate biosynthesis. © 2015 Springer-Verlag Berlin Heidelberg
KAUST Department:
Water Desalination and Reuse Research Center (WDRC)
Publisher:
Springer Science + Business Media
Journal:
Applied Microbiology and Biotechnology
Issue Date:
29-Apr-2015
DOI:
10.1007/s00253-015-6591-4
Type:
Article
ISSN:
01757598
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC)

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Yajieen
dc.contributor.authorHay, Iain D.en
dc.contributor.authorRehman, Zahid Uren
dc.contributor.authorRehm, Bernd H Aen
dc.date.accessioned2015-08-12T09:29:30Zen
dc.date.available2015-08-12T09:29:30Zen
dc.date.issued2015-04-29en
dc.identifier.issn01757598en
dc.identifier.doi10.1007/s00253-015-6591-4en
dc.identifier.urihttp://hdl.handle.net/10754/566131en
dc.description.abstractAlginates exhibit unique material properties suitable for medical and industrial applications. However, if produced by Pseudomonas aeruginosa, it is an important virulence factor in infection of cystic fibrosis patients. The alginate biosynthesis machinery is activated by c-di-GMP imparted by the inner membrane protein, MucR. Here, it was shown that MucR impairs alginate production in response to nitrate in P. aeruginosa. Subsequent site-specific mutagenesis of MucR revealed that the second MHYT sensor motif (MHYT II, amino acids 121–124) of MucR sensor domain was involved in nitrate sensing. We also showed that both c-di-GMP synthesizing and degrading active sites of MucR were important for alginate production. Although nitrate and deletion of MucR impaired alginate promoter activity and global c-di-GMP levels, alginate yields were not directly correlated with alginate promoter activity or c-di-GMP levels, suggesting that nitrate and MucR modulate alginate production at a post-translational level through a localized pool of c-di-GMP. Nitrate increased pel promoter activity in the mucR mutant while in the same mutant the psl promoter activity was independent of nitrate. Nitrate and deletion of mucR did not impact on swarming motility but impaired attachment to solid surfaces. Nitrate and deletion of mucR promoted the formation of biofilms with increased thickness, cell density, and survival. Overall, this study provided insight into the functional role of MucR with respect to nitrate-mediated regulation of alginate biosynthesis. © 2015 Springer-Verlag Berlin Heidelbergen
dc.publisherSpringer Science + Business Mediaen
dc.subjectAlginate productionen
dc.subjectAlginate regulationen
dc.subjectc-di-GMPen
dc.subjectCystic fibrosisen
dc.subjectDiguanylate cyclaseen
dc.subjectNitrateen
dc.subjectPhosphodiesteraseen
dc.titleMembrane-anchored MucR mediates nitrate-dependent regulation of alginate production in Pseudomonas aeruginosaen
dc.typeArticleen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.identifier.journalApplied Microbiology and Biotechnologyen
dc.contributor.institutionInstitute of Fundamental Sciences, Massey UniversityPalmerston North, New Zealanden
dc.contributor.institutionMacDiarmid Institute for Advanced Materials and Nanotechnology, Massey UniversityPalmerston North, New Zealanden
dc.contributor.institutionDepartment of Microbiology, Monash UniversityMelbourne, VIC, Australiaen
kaust.authorRehman, Zahid Uren
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