Extraction, amplification and detection of DNA in microfluidic chip-based assays

Handle URI:
http://hdl.handle.net/10754/566108
Title:
Extraction, amplification and detection of DNA in microfluidic chip-based assays
Authors:
Wu, Jinbo; Kodzius, Rimantas ( 0000-0001-9417-8894 ) ; Cao, Wenbin; Wen, Weijia
Abstract:
This review covers three aspects of PCR-based microfluidic chip assays: sample preparation, target amplification, and product detection. We also discuss the challenges related to the miniaturization and integration of each assay and make a comparison between conventional and microfluidic schemes. In order to accomplish these essential assays without human intervention between individual steps, the micro-components for fluid manipulation become critical. We therefore summarize and discuss components such as microvalves (for fluid regulation), pumps (for fluid driving) and mixers (for blending fluids). By combining the above assays and microcomponents, DNA testing of multi-step bio-reactions in microfluidic chips may be achieved with minimal external control. The combination of assay schemes with the use of micro-components also leads to rapid methods for DNA testing via multi-step bioreactions. Contains 259 references.
KAUST Department:
Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Publisher:
Springer Science + Business Media
Journal:
Microchimica Acta
Issue Date:
20-Dec-2013
DOI:
10.1007/s00604-013-1140-2
Type:
Article
ISSN:
00263672
Sponsors:
The authors acknowledge the financial support provided by the Hong Kong Research Grants Council Grant No. HKUST604710, 605411. This publication is based on work partially supported by Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST).
Appears in Collections:
Articles; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWu, Jinboen
dc.contributor.authorKodzius, Rimantasen
dc.contributor.authorCao, Wenbinen
dc.contributor.authorWen, Weijiaen
dc.date.accessioned2015-08-12T09:28:33Zen
dc.date.available2015-08-12T09:28:33Zen
dc.date.issued2013-12-20en
dc.identifier.issn00263672en
dc.identifier.doi10.1007/s00604-013-1140-2en
dc.identifier.urihttp://hdl.handle.net/10754/566108en
dc.description.abstractThis review covers three aspects of PCR-based microfluidic chip assays: sample preparation, target amplification, and product detection. We also discuss the challenges related to the miniaturization and integration of each assay and make a comparison between conventional and microfluidic schemes. In order to accomplish these essential assays without human intervention between individual steps, the micro-components for fluid manipulation become critical. We therefore summarize and discuss components such as microvalves (for fluid regulation), pumps (for fluid driving) and mixers (for blending fluids). By combining the above assays and microcomponents, DNA testing of multi-step bio-reactions in microfluidic chips may be achieved with minimal external control. The combination of assay schemes with the use of micro-components also leads to rapid methods for DNA testing via multi-step bioreactions. Contains 259 references.en
dc.description.sponsorshipThe authors acknowledge the financial support provided by the Hong Kong Research Grants Council Grant No. HKUST604710, 605411. This publication is based on work partially supported by Award No. SA-C0040/UK-C0016 made by King Abdullah University of Science and Technology (KAUST).en
dc.publisherSpringer Science + Business Mediaen
dc.subjectChipen
dc.subjectDNAen
dc.subjectMicrofluidicsen
dc.subjectMicromixeren
dc.subjectMicropumpen
dc.subjectMicrovalveen
dc.subjectPoint-of-care testingen
dc.subjectPolymerase chain reactionen
dc.titleExtraction, amplification and detection of DNA in microfluidic chip-based assaysen
dc.typeArticleen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.identifier.journalMicrochimica Actaen
dc.contributor.institutionDepartment of Physics, The Hong Kong University of Science and TechnologyKowloon, Clear Water Bay, Hong Kongen
kaust.authorKodzius, Rimantasen
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