Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae

Handle URI:
http://hdl.handle.net/10754/566047
Title:
Transcription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiae
Authors:
Aouida, Mustapha ( 0000-0001-7945-5320 ) ; Li, Lixin; Mahjoub, Ali ( 0000-0002-9510-3218 ) ; Alshareef, Sahar ( 0000-0002-0377-1113 ) ; Ali, Zahir ( 0000-0002-7814-8908 ) ; Piatek, Agnieszka Anna ( 0000-0001-9587-6360 ) ; Mahfouz, Magdy M. ( 0000-0002-0616-6365 )
Abstract:
Targeted engineering of microbial genomes holds much promise for diverse biotechnological applications. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/Cas9 systems are capable of efficiently editing microbial genomes, including that of Saccharomyces cerevisiae. Here, we demonstrate the use of TALENs to edit the genome of S.cerevisiae with the aim of inducing the overproduction of fatty acids. Heterodimeric TALENs were designed to simultaneously edit the FAA1 and FAA4 genes encoding acyl-CoA synthetases in S.cerevisiae. Functional yeast double knockouts generated using these TALENs over-produce large amounts of free fatty acids into the cell. This study demonstrates the use of TALENs for targeted engineering of yeast and demonstrates that this technology can be used to stimulate the enhanced production of free fatty acids, which are potential substrates for biofuel production. This proof-of-principle study extends the utility of TALENs as excellent genome editing tools and highlights their potential use for metabolic engineering of yeast and other organisms, such as microalgae and plants, for biofuel production. © 2015 The Society for Biotechnology, Japan.
KAUST Department:
Center for Desert Agriculture
Publisher:
Elsevier BV
Journal:
Journal of Bioscience and Bioengineering
Issue Date:
Apr-2015
DOI:
10.1016/j.jbiosc.2015.02.017
Type:
Article
ISSN:
13891723
Appears in Collections:
Articles; Center for Desert Agriculture

Full metadata record

DC FieldValue Language
dc.contributor.authorAouida, Mustaphaen
dc.contributor.authorLi, Lixinen
dc.contributor.authorMahjoub, Alien
dc.contributor.authorAlshareef, Saharen
dc.contributor.authorAli, Zahiren
dc.contributor.authorPiatek, Agnieszka Annaen
dc.contributor.authorMahfouz, Magdy M.en
dc.date.accessioned2015-08-12T09:00:57Zen
dc.date.available2015-08-12T09:00:57Zen
dc.date.issued2015-04en
dc.identifier.issn13891723en
dc.identifier.doi10.1016/j.jbiosc.2015.02.017en
dc.identifier.urihttp://hdl.handle.net/10754/566047en
dc.description.abstractTargeted engineering of microbial genomes holds much promise for diverse biotechnological applications. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/Cas9 systems are capable of efficiently editing microbial genomes, including that of Saccharomyces cerevisiae. Here, we demonstrate the use of TALENs to edit the genome of S.cerevisiae with the aim of inducing the overproduction of fatty acids. Heterodimeric TALENs were designed to simultaneously edit the FAA1 and FAA4 genes encoding acyl-CoA synthetases in S.cerevisiae. Functional yeast double knockouts generated using these TALENs over-produce large amounts of free fatty acids into the cell. This study demonstrates the use of TALENs for targeted engineering of yeast and demonstrates that this technology can be used to stimulate the enhanced production of free fatty acids, which are potential substrates for biofuel production. This proof-of-principle study extends the utility of TALENs as excellent genome editing tools and highlights their potential use for metabolic engineering of yeast and other organisms, such as microalgae and plants, for biofuel production. © 2015 The Society for Biotechnology, Japan.en
dc.publisherElsevier BVen
dc.subjectBiofuel productionen
dc.subjectFree fatty acidsen
dc.subjectGenome engineeringen
dc.subjectMetabolic engineeringen
dc.subjectSynthetic biologyen
dc.subjectTranscription activator-like effector nucleasesen
dc.titleTranscription activator-like effector nucleases mediated metabolic engineering for enhanced fatty acids production in Saccharomyces cerevisiaeen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.identifier.journalJournal of Bioscience and Bioengineeringen
kaust.authorAouida, Mustaphaen
kaust.authorLi, Lixinen
kaust.authorMahjoub, Alien
kaust.authorAli, Zahiren
kaust.authorMahfouz, Magdy M.en
kaust.authorAlshareef, Saharen
kaust.authorPiatek, Agnieszka Annaen
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