Microbial Community Composition and Ultrastructure of Granules from a Full-Scale Anammox Reactor

Handle URI:
http://hdl.handle.net/10754/565996
Title:
Microbial Community Composition and Ultrastructure of Granules from a Full-Scale Anammox Reactor
Authors:
Gonzalez-Gil, Graciela; Sougrat, Rachid; Behzad, Ali Reza; Lens, Piet Nl L; Saikaly, Pascal ( 0000-0001-7678-3986 )
Abstract:
Granules in anammox reactors contain besides anammox bacteria other microbial communities whose identity and relationship with the anammox bacteria are not well understood. High calcium concentrations are often supplied to anammox reactors to obtain sufficient bacterial aggregation and biomass retention. The aim of this study was to provide the first characterization of bacterial and archaeal communities in anammox granules from a full-scale anammox reactor and to explore on the possible role of calcium in such aggregates. High magnification imaging using backscattered electrons revealed that anammox bacteria may be embedded in calcium phosphate precipitates. Pyrosequencing of 16S rRNA gene fragments showed, besides anammox bacteria (Brocadiacea, 32 %), substantial numbers of heterotrophic bacteria Ignavibacteriacea (18 %) and Anaerolinea (7 %) along with heterotrophic denitrifiers Rhodocyclacea (9 %), Comamonadacea (3 %), and Shewanellacea (3 %) in the granules. It is hypothesized that these bacteria may form a network in which heterotrophic denitrifiers cooperate to achieve a well-functioning denitrification system as they can utilize the nitrate intrinsically produced by the anammox reaction. This network may provide a niche for the proliferation of archaea. Hydrogenotrophic methananogens, which scavenge the key fermentation product H2, were the most abundant archaea detected. Cells resembling the polygon-shaped denitrifying methanotroph Candidatus Methylomirabilis oxyfera were observed by electron microscopy. It is hypothesized that the anammox process in a full-scale reactor triggers various reactions overall leading to efficient denitrification and a sink of carbon as biomass in anammox granules.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Water Desalination and Reuse Research Center (WDRC); Advanced Nanofabrication, Imaging and Characterization Core Lab
Publisher:
Springer Science + Business Media
Journal:
Microbial Ecology
Issue Date:
11-Dec-2014
DOI:
10.1007/s00248-014-0546-7
Type:
Article
ISSN:
00953628
Appears in Collections:
Articles; Advanced Nanofabrication, Imaging and Characterization Core Lab; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorGonzalez-Gil, Gracielaen
dc.contributor.authorSougrat, Rachiden
dc.contributor.authorBehzad, Ali Rezaen
dc.contributor.authorLens, Piet Nl Len
dc.contributor.authorSaikaly, Pascalen
dc.date.accessioned2015-08-12T08:58:39Zen
dc.date.available2015-08-12T08:58:39Zen
dc.date.issued2014-12-11en
dc.identifier.issn00953628en
dc.identifier.doi10.1007/s00248-014-0546-7en
dc.identifier.urihttp://hdl.handle.net/10754/565996en
dc.description.abstractGranules in anammox reactors contain besides anammox bacteria other microbial communities whose identity and relationship with the anammox bacteria are not well understood. High calcium concentrations are often supplied to anammox reactors to obtain sufficient bacterial aggregation and biomass retention. The aim of this study was to provide the first characterization of bacterial and archaeal communities in anammox granules from a full-scale anammox reactor and to explore on the possible role of calcium in such aggregates. High magnification imaging using backscattered electrons revealed that anammox bacteria may be embedded in calcium phosphate precipitates. Pyrosequencing of 16S rRNA gene fragments showed, besides anammox bacteria (Brocadiacea, 32 %), substantial numbers of heterotrophic bacteria Ignavibacteriacea (18 %) and Anaerolinea (7 %) along with heterotrophic denitrifiers Rhodocyclacea (9 %), Comamonadacea (3 %), and Shewanellacea (3 %) in the granules. It is hypothesized that these bacteria may form a network in which heterotrophic denitrifiers cooperate to achieve a well-functioning denitrification system as they can utilize the nitrate intrinsically produced by the anammox reaction. This network may provide a niche for the proliferation of archaea. Hydrogenotrophic methananogens, which scavenge the key fermentation product H2, were the most abundant archaea detected. Cells resembling the polygon-shaped denitrifying methanotroph Candidatus Methylomirabilis oxyfera were observed by electron microscopy. It is hypothesized that the anammox process in a full-scale reactor triggers various reactions overall leading to efficient denitrification and a sink of carbon as biomass in anammox granules.en
dc.publisherSpringer Science + Business Mediaen
dc.subjectAnammoxen
dc.subjectAutotrophic denitrificationen
dc.subjectGranular sludgeen
dc.subjectGranulesen
dc.subjectHeterotrophic denitrificationen
dc.subjectMicrobial aggregatesen
dc.subjectPolygon-shaped bacteriaen
dc.subjectPyrosequencingen
dc.titleMicrobial Community Composition and Ultrastructure of Granules from a Full-Scale Anammox Reactoren
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentAdvanced Nanofabrication, Imaging and Characterization Core Laben
dc.identifier.journalMicrobial Ecologyen
dc.contributor.institutionUNESCO–IHEDelft, Netherlandsen
kaust.authorSougrat, Rachiden
kaust.authorBehzad, Ali Rezaen
kaust.authorSaikaly, Pascalen
kaust.authorGonzalez-Gil, Gracielaen
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