Conversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonads

Handle URI:
http://hdl.handle.net/10754/565965
Title:
Conversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonads
Authors:
Gertler, Christoph; Bargiela, Rafael; Mapelli, Francesca; Han, Xifang; Chen, Jianwei; Hai, Tran; Amer, Ranya A.; Mahjoubi, Mouna; Malkawi, Hanan Issa; Magagnini, Mirko; Cherif, Ameur; Abdel-Fattah, Yasser Refaat; Kalogerakis, Nicolas E.; Daffonchio, Daniele ( 0000-0003-0947-925X ) ; Ferrer, Manuel; Golyshin, Peter N.
Abstract:
Uric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria. © 2015 Springer Science+Business Media New York
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Springer Science + Business Media
Journal:
Microbial Ecology
Issue Date:
29-Apr-2015
DOI:
10.1007/s00248-015-0606-7
Type:
Article
Description:
An erratum to this article (correcting the affiliation information for one author) can be found at http://​dx.​doi.​org/​10.​1007/​s00248-015-0631-6.
ISSN:
00953628
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorGertler, Christophen
dc.contributor.authorBargiela, Rafaelen
dc.contributor.authorMapelli, Francescaen
dc.contributor.authorHan, Xifangen
dc.contributor.authorChen, Jianweien
dc.contributor.authorHai, Tranen
dc.contributor.authorAmer, Ranya A.en
dc.contributor.authorMahjoubi, Mounaen
dc.contributor.authorMalkawi, Hanan Issaen
dc.contributor.authorMagagnini, Mirkoen
dc.contributor.authorCherif, Ameuren
dc.contributor.authorAbdel-Fattah, Yasser Refaaten
dc.contributor.authorKalogerakis, Nicolas E.en
dc.contributor.authorDaffonchio, Danieleen
dc.contributor.authorFerrer, Manuelen
dc.contributor.authorGolyshin, Peter N.en
dc.date.accessioned2015-08-12T08:57:13Zen
dc.date.available2015-08-12T08:57:13Zen
dc.date.issued2015-04-29en
dc.identifier.issn00953628en
dc.identifier.doi10.1007/s00248-015-0606-7en
dc.identifier.urihttp://hdl.handle.net/10754/565965en
dc.descriptionAn erratum to this article (correcting the affiliation information for one author) can be found at http://​dx.​doi.​org/​10.​1007/​s00248-015-0631-6.en
dc.description.abstractUric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria. © 2015 Springer Science+Business Media New Yorken
dc.publisherSpringer Science + Business Mediaen
dc.subjectAlcanivoraxen
dc.subjectBioremediationen
dc.subjectCrude oil degradationen
dc.titleConversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonadsen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalMicrobial Ecologyen
dc.eprint.versionPost-printen
dc.contributor.institutionSchool of Biological Sciences, Environment Centre Wales, Bangor UniversityBangor, Gwynedd, United Kingdomen
dc.contributor.institutionConsejo Superior de Investigaciones Científicas (CSIC), Institute of CatalysisMadrid, Spainen
dc.contributor.institutionDepartment of Food, Environment and Nutritional Sciences (DeFENS), University of Milan, via Celoria 2Milan, Italyen
dc.contributor.institutionBGI Tech Solutions Co., Ltd, Main Building, Beishan Industrial ZoneShenzhen, Yantian District, Chinaen
dc.contributor.institutionGenetic Engineering and Biotechnology Research Institute, City for Scientific Research & Technology ApplicationsAlexandria, Egypten
dc.contributor.institutionHighe Higher Institute for Biotechnology, Biotechpole of Sidi Thabet, University of Manouba, LR11ES31Sidi Thabet, Ariana, Tunisiaen
dc.contributor.institutionDeanship of Research & Doctoral Studies, Hamdan Bin Mohammad Smart UniversityDubai, United Arab Emiratesen
dc.contributor.institutionEcoTechSystems Ltd.Ancona, Italyen
dc.contributor.institutionSchool of Environmental Engineering, Technical University of CreteChania, Greeceen
dc.contributor.institutionFriedrich-Loeffler-Institut - Federal research Institute for Animal Health, Institute of Novel and Emerging Diseases, Südufer 10Greifswald, Insel Riems, Germanyen
kaust.authorDaffonchio, Danieleen
kaust.authorMapelli, Francescaen
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