Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites

Handle URI:
http://hdl.handle.net/10754/565962
Title:
Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites
Authors:
Woo, Yong; Ansari, Hifzur Rahman ( 0000-0002-0646-1743 ) ; Otto, Thomas D.; Linger, Christen M K; Olisko, Martin K.; Michálek, Jan; Saxena, Alka; Shanmugam, Dhanasekaran; Tayyrov, Annageldi; Veluchamy, Alaguraj; Ali, Shahjahan; Bernal, Axel; Del Campo, Javier; Cihlář, Jaromír; Flegontov, Pavel; Gornik, Sebastian G.; Hajdušková, Eva; Horák, Aleš; Janouškovec, Jan; Katris, Nicholas J.; Mast, Fred D.; Miranda-Saavedra, Diego; Mourier, Tobias; Naeem, Raeece ( 0000-0003-1734-1007 ) ; Nair, Mridul; Panigrahi, Aswini Kumar; Rawlings, Neil D.; Padron Regalado, Eriko; Ramaprasad, Abhinay ( 0000-0001-9372-5526 ) ; Samad, Nadira; Tomčala, Aleš; Wilkes, Jon; Neafsey, Daniel E.; Doerig, Christian; Bowler, Chris; Keeling, Patrick J.; Roos, David S.; Dacks, Joel B.; Templeton, Thomas J.; Waller, Ross F.; Lukeš, Julius; Oborník, Miroslav; Pain, Arnab ( 0000-0002-1755-2819 )
Abstract:
The eukaryotic phylum Apicomplexa encompasses thousands of obligate intracellular parasites of humans and animals with immense socio-economic and health impacts. We sequenced nuclear genomes of Chromera velia and Vitrella brassicaformis, free-living non-parasitic photosynthetic algae closely related to apicomplexans. Proteins from key metabolic pathways and from the endomembrane trafficking systems associated with a free-living lifestyle have been progressively and non-randomly lost during adaptation to parasitism. The free-living ancestor contained a broad repertoire of genes many of which were repurposed for parasitic processes, such as extracellular proteins, components of a motility apparatus, and DNA- and RNA-binding protein families. Based on transcriptome analyses across 36 environmental conditions, Chromera orthologs of apicomplexan invasion-related motility genes were co-regulated with genes encoding the flagellar apparatus, supporting the functional contribution of flagella to the evolution of invasion machinery. This study provides insights into how obligate parasites with diverse life strategies arose from a once free-living phototrophic marine alga. © Woo et al.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Biosciences Core Lab; Pathogen Genomics Laboratory; Bioscience Program; Core Labs; Computational Bioscience Research Center (CBRC)
Publisher:
eLife Sciences Organisation, Ltd.
Journal:
eLife
Issue Date:
15-Jul-2015
DOI:
10.7554/eLife.06974
Type:
Article
ISSN:
2050-084X
Appears in Collections:
Articles; Bioscience Program; Biosciences Core Lab; Computational Bioscience Research Center (CBRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWoo, Yongen
dc.contributor.authorAnsari, Hifzur Rahmanen
dc.contributor.authorOtto, Thomas D.en
dc.contributor.authorLinger, Christen M Ken
dc.contributor.authorOlisko, Martin K.en
dc.contributor.authorMichálek, Janen
dc.contributor.authorSaxena, Alkaen
dc.contributor.authorShanmugam, Dhanasekaranen
dc.contributor.authorTayyrov, Annageldien
dc.contributor.authorVeluchamy, Alagurajen
dc.contributor.authorAli, Shahjahanen
dc.contributor.authorBernal, Axelen
dc.contributor.authorDel Campo, Javieren
dc.contributor.authorCihlář, Jaromíren
dc.contributor.authorFlegontov, Pavelen
dc.contributor.authorGornik, Sebastian G.en
dc.contributor.authorHajdušková, Evaen
dc.contributor.authorHorák, Alešen
dc.contributor.authorJanouškovec, Janen
dc.contributor.authorKatris, Nicholas J.en
dc.contributor.authorMast, Fred D.en
dc.contributor.authorMiranda-Saavedra, Diegoen
dc.contributor.authorMourier, Tobiasen
dc.contributor.authorNaeem, Raeeceen
dc.contributor.authorNair, Mridulen
dc.contributor.authorPanigrahi, Aswini Kumaren
dc.contributor.authorRawlings, Neil D.en
dc.contributor.authorPadron Regalado, Erikoen
dc.contributor.authorRamaprasad, Abhinayen
dc.contributor.authorSamad, Nadiraen
dc.contributor.authorTomčala, Alešen
dc.contributor.authorWilkes, Jonen
dc.contributor.authorNeafsey, Daniel E.en
dc.contributor.authorDoerig, Christianen
dc.contributor.authorBowler, Chrisen
dc.contributor.authorKeeling, Patrick J.en
dc.contributor.authorRoos, David S.en
dc.contributor.authorDacks, Joel B.en
dc.contributor.authorTempleton, Thomas J.en
dc.contributor.authorWaller, Ross F.en
dc.contributor.authorLukeš, Juliusen
dc.contributor.authorOborník, Miroslaven
dc.contributor.authorPain, Arnaben
dc.date.accessioned2015-08-12T08:57:03Zen
dc.date.available2015-08-12T08:57:03Zen
dc.date.issued2015-07-15en
dc.identifier.issn2050-084Xen
dc.identifier.doi10.7554/eLife.06974en
dc.identifier.urihttp://hdl.handle.net/10754/565962en
dc.description.abstractThe eukaryotic phylum Apicomplexa encompasses thousands of obligate intracellular parasites of humans and animals with immense socio-economic and health impacts. We sequenced nuclear genomes of Chromera velia and Vitrella brassicaformis, free-living non-parasitic photosynthetic algae closely related to apicomplexans. Proteins from key metabolic pathways and from the endomembrane trafficking systems associated with a free-living lifestyle have been progressively and non-randomly lost during adaptation to parasitism. The free-living ancestor contained a broad repertoire of genes many of which were repurposed for parasitic processes, such as extracellular proteins, components of a motility apparatus, and DNA- and RNA-binding protein families. Based on transcriptome analyses across 36 environmental conditions, Chromera orthologs of apicomplexan invasion-related motility genes were co-regulated with genes encoding the flagellar apparatus, supporting the functional contribution of flagella to the evolution of invasion machinery. This study provides insights into how obligate parasites with diverse life strategies arose from a once free-living phototrophic marine alga. © Woo et al.en
dc.publishereLife Sciences Organisation, Ltd.en
dc.titleChromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasitesen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentPathogen Genomics Laboratoryen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentCore Labsen
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.identifier.journaleLifeen
kaust.authorAnsari, Hifzur Rahmanen
kaust.authorAli, Shahjahanen
kaust.authorNaeem, Raeeceen
kaust.authorNair, Mridulen
kaust.authorPanigrahi, Aswini Kumaren
kaust.authorRamaprasad, Abhinayen
kaust.authorPain, Arnaben
kaust.authorWoo, Yongen
kaust.authorSaxena, Alkaen
kaust.authorTayyrov, Annageldien
kaust.authorPadron Regalado, Erikoen
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