Characterization and cross-amplification of microsatellite markers in four species of anemonefish (Pomacentridae, Amphiprion spp.)

Handle URI:
http://hdl.handle.net/10754/565960
Title:
Characterization and cross-amplification of microsatellite markers in four species of anemonefish (Pomacentridae, Amphiprion spp.)
Authors:
Bonin, Mary C.; Saenz Agudelo, Pablo; Harrison, Hugo B.; Nanninga, Gerrit B. ( 0000-0002-0134-1689 ) ; Van Der Meer, Martin H.; Mansour, Hicham; Perumal, Sadhasivam; Jones, Geoffrey P.; Berumen, Michael L. ( 0000-0003-2463-2742 )
Abstract:
Anemonefish are iconic symbols of coral reefs and have become model systems for research on larval dispersal and population connectivity in coral reef fishes. Here we present 24 novel microsatellite markers across four species of anemonefish and also test 35 previously published markers for cross-amplification on two anemonefish species in order to facilitate further research on their population genetics and phylogenetics. Novel loci were isolated from sequences derived from microsatellite-enriched or 454 GS-FLX shotgun sequence libraries developed using congeneric DNA. Primer testing successfully identified 15 new microsatellite loci for A. percula, 4 for A. melanopus, 3 for A. akindynos, and 2 for A. omanensis. These novel microsatellite loci were polymorphic with a mean of 10 ± 1.6 SE (standard error) alleles per locus and an average observed heterozygosity of 0.647 ± 0.032 SE. Reliable cross-amplification of 12 and 26 of the 35 previously published Amphiprion markers was achieved for A. melanopus and A. akindynos, respectively, suggesting that the use of markers developed from the DNA of congeners can provide a quick and cost-effective alternative to the isolation of new loci. Together, the markers presented here provide an important resource for ecological, evolutionary, and conservation genetic research on anemonefishes that will inform broader conservation and management actions for coral reef fishes. © 2015 Senckenberg Gesellschaft für Naturforschung and Springer-Verlag Berlin Heidelberg
KAUST Department:
Red Sea Research Center (RSRC); Biosciences Core Lab
Publisher:
Springer Science + Business Media
Journal:
Marine Biodiversity
Issue Date:
9-Apr-2015
DOI:
10.1007/s12526-015-0336-6
Type:
Article
ISSN:
18671616
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Biosciences Core Lab

Full metadata record

DC FieldValue Language
dc.contributor.authorBonin, Mary C.en
dc.contributor.authorSaenz Agudelo, Pabloen
dc.contributor.authorHarrison, Hugo B.en
dc.contributor.authorNanninga, Gerrit B.en
dc.contributor.authorVan Der Meer, Martin H.en
dc.contributor.authorMansour, Hichamen
dc.contributor.authorPerumal, Sadhasivamen
dc.contributor.authorJones, Geoffrey P.en
dc.contributor.authorBerumen, Michael L.en
dc.date.accessioned2015-08-12T08:56:56Zen
dc.date.available2015-08-12T08:56:56Zen
dc.date.issued2015-04-09en
dc.identifier.issn18671616en
dc.identifier.doi10.1007/s12526-015-0336-6en
dc.identifier.urihttp://hdl.handle.net/10754/565960en
dc.description.abstractAnemonefish are iconic symbols of coral reefs and have become model systems for research on larval dispersal and population connectivity in coral reef fishes. Here we present 24 novel microsatellite markers across four species of anemonefish and also test 35 previously published markers for cross-amplification on two anemonefish species in order to facilitate further research on their population genetics and phylogenetics. Novel loci were isolated from sequences derived from microsatellite-enriched or 454 GS-FLX shotgun sequence libraries developed using congeneric DNA. Primer testing successfully identified 15 new microsatellite loci for A. percula, 4 for A. melanopus, 3 for A. akindynos, and 2 for A. omanensis. These novel microsatellite loci were polymorphic with a mean of 10 ± 1.6 SE (standard error) alleles per locus and an average observed heterozygosity of 0.647 ± 0.032 SE. Reliable cross-amplification of 12 and 26 of the 35 previously published Amphiprion markers was achieved for A. melanopus and A. akindynos, respectively, suggesting that the use of markers developed from the DNA of congeners can provide a quick and cost-effective alternative to the isolation of new loci. Together, the markers presented here provide an important resource for ecological, evolutionary, and conservation genetic research on anemonefishes that will inform broader conservation and management actions for coral reef fishes. © 2015 Senckenberg Gesellschaft für Naturforschung and Springer-Verlag Berlin Heidelbergen
dc.publisherSpringer Science + Business Mediaen
dc.subjectAmphiprionen
dc.subjectClownfishen
dc.subjectCoral reefsen
dc.subjectMicrosatelliteen
dc.subjectPopulation geneticsen
dc.titleCharacterization and cross-amplification of microsatellite markers in four species of anemonefish (Pomacentridae, Amphiprion spp.)en
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiosciences Core Laben
dc.identifier.journalMarine Biodiversityen
dc.contributor.institutionARC Centre of Excellence for Coral Reef Studies, James Cook UniversityTownsville, QLD, Australiaen
dc.contributor.institutionCollege of Marine and Environmental Sciences, James Cook UniversityTownsville, QLD, Australiaen
dc.contributor.institutionFacultad de Ciencias, Instituto de Ciencias Ambientales y Evolutivas, Universidad Austral de ChileValdivia, Chileen
kaust.authorSaenz Agudelo, Pabloen
kaust.authorNanninga, Gerrit B.en
kaust.authorMansour, Hichamen
kaust.authorBerumen, Michael L.en
kaust.authorPerumal, Sadhasivamen
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