Diverse Reductive Dehalogenases Are Associated with Clostridiales-Enriched Microcosms Dechlorinating 1,2-Dichloroethane

Handle URI:
http://hdl.handle.net/10754/565936
Title:
Diverse Reductive Dehalogenases Are Associated with Clostridiales-Enriched Microcosms Dechlorinating 1,2-Dichloroethane
Authors:
Merlino, Giuseppe ( 0000-0001-5182-2301 ) ; Balloi, Annalisa ( 0000-0002-1889-0422 ) ; Marzorati, Massimo; Mapelli, Francesca ( 0000-0002-2928-6538 ) ; Rizzi, Aurora; Lavazza, Davide; de Ferra, Francesca; Carpani, Giovanna; Daffonchio, Daniele ( 0000-0003-0947-925X )
Abstract:
The achievement of successful biostimulation of active microbiomes for the cleanup of a polluted site is strictly dependent on the knowledge of the key microorganisms equipped with the relevant catabolic genes responsible for the degradation process. In this work, we present the characterization of the bacterial community developed in anaerobic microcosms after biostimulation with the electron donor lactate of groundwater polluted with 1,2-dichloroethane (1,2-DCA). Through a multilevel analysis, we have assessed (i) the structural analysis of the bacterial community; (ii) the identification of putative dehalorespiring bacteria; (iii) the characterization of functional genes encoding for putative 1,2-DCA reductive dehalogenases (RDs). Following the biostimulation treatment, the structure of the bacterial community underwent a notable change of the main phylotypes, with the enrichment of representatives of the order Clostridiales . Through PCR targeting conserved regions within known RD genes, four novel variants of RDs previously associated with the reductive dechlorination of 1,2-DCA were identified in the metagenome of the Clostridiales-dominated bacterial community.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Diverse Reductive Dehalogenases Are Associated with Clostridiales-Enriched Microcosms Dechlorinating 1,2-Dichloroethane 2015, 2015:1 BioMed Research International
Publisher:
Hindawi Publishing Corporation
Journal:
BioMed Research International
Issue Date:
6-Mar-2015
DOI:
10.1155/2015/242856
PubMed ID:
26273600
Type:
Article
ISSN:
2314-6133; 2314-6141
Additional Links:
http://www.hindawi.com/journals/bmri/2015/242856/
Appears in Collections:
Articles; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMerlino, Giuseppeen
dc.contributor.authorBalloi, Annalisaen
dc.contributor.authorMarzorati, Massimoen
dc.contributor.authorMapelli, Francescaen
dc.contributor.authorRizzi, Auroraen
dc.contributor.authorLavazza, Davideen
dc.contributor.authorde Ferra, Francescaen
dc.contributor.authorCarpani, Giovannaen
dc.contributor.authorDaffonchio, Danieleen
dc.date.accessioned2015-08-12T08:49:39Zen
dc.date.available2015-08-12T08:49:39Zen
dc.date.issued2015-03-06en
dc.identifier.citationDiverse Reductive Dehalogenases Are Associated with Clostridiales-Enriched Microcosms Dechlorinating 1,2-Dichloroethane 2015, 2015:1 BioMed Research Internationalen
dc.identifier.issn2314-6133en
dc.identifier.issn2314-6141en
dc.identifier.pmid26273600-
dc.identifier.doi10.1155/2015/242856en
dc.identifier.urihttp://hdl.handle.net/10754/565936en
dc.description.abstractThe achievement of successful biostimulation of active microbiomes for the cleanup of a polluted site is strictly dependent on the knowledge of the key microorganisms equipped with the relevant catabolic genes responsible for the degradation process. In this work, we present the characterization of the bacterial community developed in anaerobic microcosms after biostimulation with the electron donor lactate of groundwater polluted with 1,2-dichloroethane (1,2-DCA). Through a multilevel analysis, we have assessed (i) the structural analysis of the bacterial community; (ii) the identification of putative dehalorespiring bacteria; (iii) the characterization of functional genes encoding for putative 1,2-DCA reductive dehalogenases (RDs). Following the biostimulation treatment, the structure of the bacterial community underwent a notable change of the main phylotypes, with the enrichment of representatives of the order Clostridiales . Through PCR targeting conserved regions within known RD genes, four novel variants of RDs previously associated with the reductive dechlorination of 1,2-DCA were identified in the metagenome of the Clostridiales-dominated bacterial community.en
dc.language.isoenen
dc.publisherHindawi Publishing Corporationen
dc.relation.urlhttp://www.hindawi.com/journals/bmri/2015/242856/en
dc.rightsThis is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/en
dc.titleDiverse Reductive Dehalogenases Are Associated with Clostridiales-Enriched Microcosms Dechlorinating 1,2-Dichloroethaneen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalBioMed Research Internationalen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDepartment of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, 20133 Milan, Italyen
dc.contributor.institutionLaboratory for Microbial Ecology and Technology (LabMET), Ghent University, 9000 Ghent, Belgiumen
dc.contributor.institutionResearch Center for Non-Conventional Energy, Istituto Eni Donegani, Environmental Technologies, 20097 San Donato Milanese, Italyen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorDaffonchio, Danieleen
kaust.authorMerlino, Giuseppeen

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