Analysis of Globodera rostochiensis effectors reveals conserved functions of SPRYSEC proteins in suppressing and eliciting plant immune responses

Handle URI:
http://hdl.handle.net/10754/565913
Title:
Analysis of Globodera rostochiensis effectors reveals conserved functions of SPRYSEC proteins in suppressing and eliciting plant immune responses
Authors:
Ali, Shawkat ( 0000-0003-3282-3186 ) ; Magne, Maxime; Chen, Shiyan; Obradovic, Natasa; Jamshaid, Lubna; Wang, Xiaohong; Bélair, Guy; Moffett, Peter
Abstract:
Potato cyst nematodes (PCNs), including Globodera rostochiensis (Woll.), are important pests of potato. Plant parasitic nematodes produce multiple effector proteins, secreted from their stylets, to successfully infect their hosts. These include proteins delivered to the apoplast and to the host cytoplasm. A number of effectors from G. rostochiensis predicted to be delivered to the host cytoplasm have been identified, including several belonging to the secreted SPRY domain (SPRYSEC) family. SPRYSEC proteins are unique to members of the genus Globodera and have been implicated in both the induction and the repression of host defense responses. We have tested the properties of six different G. rostochiensis SPRYSEC proteins by expressing them in Nicotiana benthamiana and N. tabacum. We have found that all SPRYSEC proteins tested are able to suppress defense responses induced by NB-LRR proteins as well as cell death induced by elicitors, suggesting that defense repression is a common characteristic of members of this effector protein family. At the same time, GrSPRYSEC-15 elicited a defense responses in N. tabacum, which was found to be resistant to a virus expressing GrSPRYSEC-15. These results suggest that SPRYSEC proteins may possess characteristics that allow them to be recognized by the plant immune system.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division
Citation:
Analysis of Globodera rostochiensis effectors reveals conserved functions of SPRYSEC proteins in suppressing and eliciting plant immune responses 2015, 6 Frontiers in Plant Science
Publisher:
Frontiers Media SA
Journal:
Frontiers in Plant Science
Issue Date:
11-Aug-2015
DOI:
10.3389/fpls.2015.00623
Type:
Article
ISSN:
1664-462X
Additional Links:
http://journal.frontiersin.org/article/10.3389/fpls.2015.00623
Appears in Collections:
Articles; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorAli, Shawkaten
dc.contributor.authorMagne, Maximeen
dc.contributor.authorChen, Shiyanen
dc.contributor.authorObradovic, Natasaen
dc.contributor.authorJamshaid, Lubnaen
dc.contributor.authorWang, Xiaohongen
dc.contributor.authorBélair, Guyen
dc.contributor.authorMoffett, Peteren
dc.date.accessioned2015-08-12T08:48:26Zen
dc.date.available2015-08-12T08:48:26Zen
dc.date.issued2015-08-11en
dc.identifier.citationAnalysis of Globodera rostochiensis effectors reveals conserved functions of SPRYSEC proteins in suppressing and eliciting plant immune responses 2015, 6 Frontiers in Plant Scienceen
dc.identifier.issn1664-462Xen
dc.identifier.doi10.3389/fpls.2015.00623en
dc.identifier.urihttp://hdl.handle.net/10754/565913en
dc.description.abstractPotato cyst nematodes (PCNs), including Globodera rostochiensis (Woll.), are important pests of potato. Plant parasitic nematodes produce multiple effector proteins, secreted from their stylets, to successfully infect their hosts. These include proteins delivered to the apoplast and to the host cytoplasm. A number of effectors from G. rostochiensis predicted to be delivered to the host cytoplasm have been identified, including several belonging to the secreted SPRY domain (SPRYSEC) family. SPRYSEC proteins are unique to members of the genus Globodera and have been implicated in both the induction and the repression of host defense responses. We have tested the properties of six different G. rostochiensis SPRYSEC proteins by expressing them in Nicotiana benthamiana and N. tabacum. We have found that all SPRYSEC proteins tested are able to suppress defense responses induced by NB-LRR proteins as well as cell death induced by elicitors, suggesting that defense repression is a common characteristic of members of this effector protein family. At the same time, GrSPRYSEC-15 elicited a defense responses in N. tabacum, which was found to be resistant to a virus expressing GrSPRYSEC-15. These results suggest that SPRYSEC proteins may possess characteristics that allow them to be recognized by the plant immune system.en
dc.language.isoenen
dc.publisherFrontiers Media SAen
dc.relation.urlhttp://journal.frontiersin.org/article/10.3389/fpls.2015.00623en
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.http://creativecommons.org/licenses/by/4.0/en
dc.subjectGloboderaen
dc.subjectplant-parasitic nematodeen
dc.subjectcyst nematodesen
dc.subjectNB-LRR proteinsen
dc.subjecteffector proteinsen
dc.subjectPAMP-triggered immunity (PTI)en
dc.subjectSPRYSECen
dc.titleAnalysis of Globodera rostochiensis effectors reveals conserved functions of SPRYSEC proteins in suppressing and eliciting plant immune responsesen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalFrontiers in Plant Scienceen
dc.eprint.versionPublisher's Version/PDFen
dc.contributor.institutionDépartement de Biologie, Université de Sherbrooke, Sherbrooke, QC, Canadaen
dc.contributor.institutionHorticulture R & D Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, QC, Canadaen
dc.contributor.institutionSchool of Integrative Plant Science, Cornell University, Ithaca, NY, USAen
dc.contributor.institutionUS Department of Agriculture, Robert W. Holley Center for Agriculture and Health, Agricultural Research Service, Ithaca, NY, USAen
dc.contributor.affiliationKing Abdullah University of Science and Technology (KAUST)en
kaust.authorAli, Shawkaten
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