Ovarian Hormone Estrone Glucuronide (E1G) quantification-impedimetric electrochemical spectroscopy approach

Handle URI:
http://hdl.handle.net/10754/564831
Title:
Ovarian Hormone Estrone Glucuronide (E1G) quantification-impedimetric electrochemical spectroscopy approach
Authors:
Zia, Asif I.; Mukhopadhyay, Subhas Chandra; Yu, Paklam; Al-Bahadly, Ibrahim H.; Yudhana, Anton; Gooneratne, Chinthaka Pasan; Kosel, Jürgen ( 0000-0002-8998-8275 )
Abstract:
A study was conducted on detection and concentration measurement of estrone glucuronide (E1G), an important metabolite of the ovarian hormone estradiol, by using Electrochemical Impedance Spectroscopy (EIS) technique. A miniature planar Inter-digital (ID) capacitive sensor fabricated on single crystal silicon substrate with sputtered gold electrodes coupled with EIS was used to measure conductivity, permeability and dielectric properties of the said hormone metabolite. A thin film of Silicon Nitride (50 um) was coated on the sensor as passivation layer to avoid Faradic currents through the sensor. Impedance spectrums were obtained with various concentrations of E1G in buffer solution by exposing the samples to electrical perturbations at certain frequency range. Relationship of sample conductance with E1G concentration was studied on basis Randle's equivalent circuit model and results were analyzed to deduce Constant Phase Equivalent (CPE) Circuit model in order to evaluate the double layer capacitance produced at the solution-electrode interface due to kinetic processes taking place in the electrochemical cell. The sensitivity of the sensor was evaluated against concentration. The result analysis confirmed that fabricated ID sensor together with EIS can provide a rapid and successful low cost sensing system which can help a lay user to determine peak time for feminine reproductive fertility at home without submitting samples for an expensive and time consuming laboratory test. © 2013 IEEE.
KAUST Department:
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Electrical Engineering Program; Sensing, Magnetism and Microsystems Lab
Publisher:
Institute of Electrical and Electronics Engineers (IEEE)
Journal:
2013 Seventh International Conference on Sensing Technology (ICST)
Conference/Event name:
2013 7th International Conference on Sensing Technology, ICST 2013
Issue Date:
Dec-2013
DOI:
10.1109/ICSensT.2013.6727610
Type:
Conference Paper
ISSN:
21568065
ISBN:
9781467352215
Appears in Collections:
Conference Papers; Electrical Engineering Program; Sensing, Magnetism and Microsystems Lab; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorZia, Asif I.en
dc.contributor.authorMukhopadhyay, Subhas Chandraen
dc.contributor.authorYu, Paklamen
dc.contributor.authorAl-Bahadly, Ibrahim H.en
dc.contributor.authorYudhana, Antonen
dc.contributor.authorGooneratne, Chinthaka Pasanen
dc.contributor.authorKosel, Jürgenen
dc.date.accessioned2015-08-04T07:17:39Zen
dc.date.available2015-08-04T07:17:39Zen
dc.date.issued2013-12en
dc.identifier.isbn9781467352215en
dc.identifier.issn21568065en
dc.identifier.doi10.1109/ICSensT.2013.6727610en
dc.identifier.urihttp://hdl.handle.net/10754/564831en
dc.description.abstractA study was conducted on detection and concentration measurement of estrone glucuronide (E1G), an important metabolite of the ovarian hormone estradiol, by using Electrochemical Impedance Spectroscopy (EIS) technique. A miniature planar Inter-digital (ID) capacitive sensor fabricated on single crystal silicon substrate with sputtered gold electrodes coupled with EIS was used to measure conductivity, permeability and dielectric properties of the said hormone metabolite. A thin film of Silicon Nitride (50 um) was coated on the sensor as passivation layer to avoid Faradic currents through the sensor. Impedance spectrums were obtained with various concentrations of E1G in buffer solution by exposing the samples to electrical perturbations at certain frequency range. Relationship of sample conductance with E1G concentration was studied on basis Randle's equivalent circuit model and results were analyzed to deduce Constant Phase Equivalent (CPE) Circuit model in order to evaluate the double layer capacitance produced at the solution-electrode interface due to kinetic processes taking place in the electrochemical cell. The sensitivity of the sensor was evaluated against concentration. The result analysis confirmed that fabricated ID sensor together with EIS can provide a rapid and successful low cost sensing system which can help a lay user to determine peak time for feminine reproductive fertility at home without submitting samples for an expensive and time consuming laboratory test. © 2013 IEEE.en
dc.publisherInstitute of Electrical and Electronics Engineers (IEEE)en
dc.subjectdielectricen
dc.subjectE1Gen
dc.subjectelectrochemicalen
dc.subjectestrone glucuronideen
dc.subjectImpedance spectroscopyen
dc.subjectInterdigitalen
dc.subjectMEMSen
dc.subjectsensing performanceen
dc.subjectsensorsen
dc.titleOvarian Hormone Estrone Glucuronide (E1G) quantification-impedimetric electrochemical spectroscopy approachen
dc.typeConference Paperen
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentElectrical Engineering Programen
dc.contributor.departmentSensing, Magnetism and Microsystems Laben
dc.identifier.journal2013 Seventh International Conference on Sensing Technology (ICST)en
dc.conference.date3 December 2013 through 5 December 2013en
dc.conference.name2013 7th International Conference on Sensing Technology, ICST 2013en
dc.conference.locationWellingtonen
dc.contributor.institutionSchool of Engineering and Advanced Technology, Massey University, Palmerston North, New Zealanden
dc.contributor.institutionDept. of Electrical Engineering, Ahmad Dahlan University, Indonesiaen
kaust.authorGooneratne, Chinthaka Pasanen
kaust.authorKosel, Jürgenen
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