A microfluidic dialysis device for complex biological mixture SERS analysis

Handle URI:
http://hdl.handle.net/10754/564194
Title:
A microfluidic dialysis device for complex biological mixture SERS analysis
Authors:
Perozziello, Gerardo; Candeloro, Patrizio; Gentile, Francesco T.; Coluccio, Maria Laura; Tallerico, Marco ( 0000-0002-6791-9068 ) ; De Grazia, Antonio ( 0000-0002-8935-7801 ) ; Nicastri, Annalisa; Perri, Angela Mena; Parrotta, Elvira; Pardeo, Francesca; Catalano, Rossella; Cuda, Giovanni; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
In this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
Aug-2015
DOI:
10.1016/j.mee.2015.02.015
Type:
Article
ISSN:
01679317
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorPerozziello, Gerardoen
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorGentile, Francesco T.en
dc.contributor.authorColuccio, Maria Lauraen
dc.contributor.authorTallerico, Marcoen
dc.contributor.authorDe Grazia, Antonioen
dc.contributor.authorNicastri, Annalisaen
dc.contributor.authorPerri, Angela Menaen
dc.contributor.authorParrotta, Elviraen
dc.contributor.authorPardeo, Francescaen
dc.contributor.authorCatalano, Rossellaen
dc.contributor.authorCuda, Giovannien
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-08-03T12:35:57Zen
dc.date.available2015-08-03T12:35:57Zen
dc.date.issued2015-08en
dc.identifier.issn01679317en
dc.identifier.doi10.1016/j.mee.2015.02.015en
dc.identifier.urihttp://hdl.handle.net/10754/564194en
dc.description.abstractIn this paper, we present a microfluidic device fabricated with a simple and inexpensive process allowing rapid filtering of peptides from a complex mixture. The polymer microfluidic device can be used for sample preparation in biological applications. The device is fabricated by micromilling and solvent assisted bonding, in which a microdialysis membrane (cut-off of 12-14 kDa) is sandwiched in between an upper and a bottom microfluidic chamber. An external frame connects the microfluidic device to external tubes, microvalves and syringe pumps. Bonding strength and interface sealing are pneumatically tested. Microfluidic protocols are also described by using the presented device to filter a sample composed of specific peptides (MW 1553.73 Da, at a concentration of 1.0 ng/μl) derived from the BRCA1 protein, a tumor-suppressor molecule which plays a pivotal role in the development of breast cancer, and albumin (MW 66.5 kDa, at a concentration of 35 μg/μl), the most represented protein in human plasma. The filtered samples coming out from the microfluidic device were subsequently deposited on a SERS (surface enhanced Raman scattering) substrate for further analysis by Raman spectroscopy. By using this approach, we were able to sort the small peptides from the bigger and highly concentrated protein albumin and to detect them by using a label-free technique at a resolution down to 1.0 ng/μl.en
dc.publisherElsevier BVen
dc.subjectComplex mixture analysisen
dc.subjectMicrofluidic filteringen
dc.subjectSERS analysisen
dc.titleA microfluidic dialysis device for complex biological mixture SERS analysisen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalMicroelectronic Engineeringen
dc.contributor.institutionBioNEM Lab, Department of Experimental and Clinical Medicine, University Magna Graecia of CatanzaroCatanzaro, Italyen
dc.contributor.institutionProteomics Lab, Department of Experimental and Clinical Medicine, University Magna Graecia of CatanzaroCatanzaro, Italyen
kaust.authorTallerico, Marcoen
kaust.authorDe Grazia, Antonioen
kaust.authorDi Fabrizio, Enzo M.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.