Photolithography and micromolding techniques for the realization of 3D polycaprolactone scaffolds for tissue engineering applications

Handle URI:
http://hdl.handle.net/10754/564178
Title:
Photolithography and micromolding techniques for the realization of 3D polycaprolactone scaffolds for tissue engineering applications
Authors:
Limongi, Tania; Schipani, Rossana ( 0000-0003-3593-6522 ) ; Di Vito, Anna; Giugni, Andrea; Francardi, Marco; Torre, Bruno; Allione, Marco ( 0000-0003-0757-9791 ) ; Miele, Ermanno; Malara, Natalia Maria; Alrasheed, Salma; Raimondo, Raffaella; Candeloro, Patrizio; Mollace, Vincenzo; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
Material science, cell biology, and engineering are all part of the research field of tissue engineering. It is the application of knowledge, methods and instrumentations of engineering and life science to the development of biocompatible solutions for repair and/or replace tissues and damaged organs. Last generation microfabrication technologies utilizing natural and synthetic biomaterials allow the realization of scaffolds resembling tissue-like structures as skin, brain, bones, muscles, cartilage and blood vessels. In this work we describe an effective and simple micromolding fabrication process allowing the realization of 3D polycaprolactone (PCL) scaffold for human neural stem cells (hNSC) culture. Scanning Electron Microscopy has been used to investigate the micro and nano features characterizing the surface of the device. Immunofluorescence analysis showed how, after seeding cells onto the substrate, healthy astrocytes grew up in a well-organized 3D network. Thus, we proposed this effective fabrication method for the production of nanopatterned PCL pillared scaffold providing a biomimetic environment for the growth of hNSC, a promising and efficient means for future applications in tissue engineering and regenerative medicine.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Biological and Environmental Sciences and Engineering (BESE) Division; Materials Science and Engineering Program
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
Jun-2015
DOI:
10.1016/j.mee.2015.02.030
Type:
Article
ISSN:
01679317
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorLimongi, Taniaen
dc.contributor.authorSchipani, Rossanaen
dc.contributor.authorDi Vito, Annaen
dc.contributor.authorGiugni, Andreaen
dc.contributor.authorFrancardi, Marcoen
dc.contributor.authorTorre, Brunoen
dc.contributor.authorAllione, Marcoen
dc.contributor.authorMiele, Ermannoen
dc.contributor.authorMalara, Natalia Mariaen
dc.contributor.authorAlrasheed, Salmaen
dc.contributor.authorRaimondo, Raffaellaen
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorMollace, Vincenzoen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-08-03T12:35:17Zen
dc.date.available2015-08-03T12:35:17Zen
dc.date.issued2015-06en
dc.identifier.issn01679317en
dc.identifier.doi10.1016/j.mee.2015.02.030en
dc.identifier.urihttp://hdl.handle.net/10754/564178en
dc.description.abstractMaterial science, cell biology, and engineering are all part of the research field of tissue engineering. It is the application of knowledge, methods and instrumentations of engineering and life science to the development of biocompatible solutions for repair and/or replace tissues and damaged organs. Last generation microfabrication technologies utilizing natural and synthetic biomaterials allow the realization of scaffolds resembling tissue-like structures as skin, brain, bones, muscles, cartilage and blood vessels. In this work we describe an effective and simple micromolding fabrication process allowing the realization of 3D polycaprolactone (PCL) scaffold for human neural stem cells (hNSC) culture. Scanning Electron Microscopy has been used to investigate the micro and nano features characterizing the surface of the device. Immunofluorescence analysis showed how, after seeding cells onto the substrate, healthy astrocytes grew up in a well-organized 3D network. Thus, we proposed this effective fabrication method for the production of nanopatterned PCL pillared scaffold providing a biomimetic environment for the growth of hNSC, a promising and efficient means for future applications in tissue engineering and regenerative medicine.en
dc.publisherElsevier BVen
dc.subjectBiocompatible substrateen
dc.subjectHuman stem cellsen
dc.subjectMicrofabrication techniqueen
dc.subjectNanostructured PCL pillarsen
dc.subjectNeural networksen
dc.titlePhotolithography and micromolding techniques for the realization of 3D polycaprolactone scaffolds for tissue engineering applicationsen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalMicroelectronic Engineeringen
dc.contributor.institutionDepartment of Experimental and Clinical Medicine, University of Catanzaro Magna Graecia, University Campus Salvatore VenutaGermaneto, Catanzaro, Italyen
dc.contributor.institutionIstituto Italiano di Tecnologia (IIT), via Morego 30Genova, Italyen
dc.contributor.institutionDepartment of Health Science, University Magna Graecia of Catanzaro, Complesso Ninì BarbieriRoccelletta di Borgia, Italyen
dc.contributor.institutionBIONEM, Department of Experimental and Clinical Medicine, University of Magna Graecia Viale EuropaGermaneto, 88100 Catanzaro, Italyen
kaust.authorLimongi, Taniaen
kaust.authorBatra, Nitin Men
kaust.authorGiugni, Andreaen
kaust.authorFrancardi, Marcoen
kaust.authorTorre, Brunoen
kaust.authorAllione, Marcoen
kaust.authorDi Fabrizio, Enzo M.en
kaust.authorAlrasheed, Salmaen
kaust.authorRaimondo, Raffaellaen
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