Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana

Handle URI:
http://hdl.handle.net/10754/564127
Title:
Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana
Authors:
Danquah, Agyemang; Zélicourt, Axel de ( 0000-0003-0797-6308 ) ; Boudsocq, Marie; Neubauer, Jorinde; Frei Dit Frey, Nicolas; Leonhardt, Nathalie; Pateyron, Stéphanie; Gwinner, Frederik; Tamby, Jean Philippe; Ortiz-Masià, Dolores; Marcote, María Jesús; Hirt, Heribert ( 0000-0003-3119-9633 ) ; Colcombet, Jean
Abstract:
Summary Abscisic acid (ABA) is a major phytohormone involved in important stress-related and developmental plant processes. Recent phosphoproteomic analyses revealed a large set of ABA-triggered phosphoproteins as putative mitogen-activated protein kinase (MAPK) targets, although the evidence for MAPKs involved in ABA signalling is still scarce. Here, we identified and reconstituted in vivo a complete ABA-activated MAPK cascade, composed of the MAP3Ks MAP3K17/18, the MAP2K MKK3 and the four C group MAPKs MPK1/2/7/14. In planta, we show that ABA activation of MPK7 is blocked in mkk3-1 and map3k17mapk3k18 plants. Coherently, both mutants exhibit hypersensitivity to ABA and altered expression of a set of ABA-dependent genes. A genetic analysis further reveals that this MAPK cascade is activated by the PYR/PYL/RCAR-SnRK2-PP2C ABA core signalling module through protein synthesis of the MAP3Ks, unveiling an atypical mechanism for MAPK activation in eukaryotes. Our work provides evidence for a role of an ABA-induced MAPK pathway in plant stress signalling. Significance Statement We report in this article the identification of a complete MAPK module, composed of MAP3K17/18, MKK3 and MPK1/2/7/14, which is activated by ABA through the ABA core signalling complex. We showed that the activation of this module requires the MAP3K protein synthesis which occurs after hours of stress treatment, suggesting that the pathway is involved in a delayed wave of cellular responses to ABA and drought. © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.
KAUST Department:
Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program
Publisher:
Wiley-Blackwell
Journal:
The Plant Journal
Issue Date:
Apr-2015
DOI:
10.1111/tpj.12808
Type:
Article
ISSN:
09607412
Sponsors:
We thank Sean Cutler, Sylvain Merlot and Jane Parker for kindly providing hab1G246D line, pyr1pyl1pyl2pyl4 mutant and pEXCS-GW-mYFP-nls plasmid, respectively. This work was supported by the Institut National de Recherche Agronomique and the Agence Nationale de la Recherche. AD was supported by the Ghanaian Ministry of Education.
Appears in Collections:
Articles; Bioscience Program; Center for Desert Agriculture; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorDanquah, Agyemangen
dc.contributor.authorZélicourt, Axel deen
dc.contributor.authorBoudsocq, Marieen
dc.contributor.authorNeubauer, Jorindeen
dc.contributor.authorFrei Dit Frey, Nicolasen
dc.contributor.authorLeonhardt, Nathalieen
dc.contributor.authorPateyron, Stéphanieen
dc.contributor.authorGwinner, Frederiken
dc.contributor.authorTamby, Jean Philippeen
dc.contributor.authorOrtiz-Masià, Doloresen
dc.contributor.authorMarcote, María Jesúsen
dc.contributor.authorHirt, Heriberten
dc.contributor.authorColcombet, Jeanen
dc.date.accessioned2015-08-03T12:33:18Zen
dc.date.available2015-08-03T12:33:18Zen
dc.date.issued2015-04en
dc.identifier.issn09607412en
dc.identifier.doi10.1111/tpj.12808en
dc.identifier.urihttp://hdl.handle.net/10754/564127en
dc.description.abstractSummary Abscisic acid (ABA) is a major phytohormone involved in important stress-related and developmental plant processes. Recent phosphoproteomic analyses revealed a large set of ABA-triggered phosphoproteins as putative mitogen-activated protein kinase (MAPK) targets, although the evidence for MAPKs involved in ABA signalling is still scarce. Here, we identified and reconstituted in vivo a complete ABA-activated MAPK cascade, composed of the MAP3Ks MAP3K17/18, the MAP2K MKK3 and the four C group MAPKs MPK1/2/7/14. In planta, we show that ABA activation of MPK7 is blocked in mkk3-1 and map3k17mapk3k18 plants. Coherently, both mutants exhibit hypersensitivity to ABA and altered expression of a set of ABA-dependent genes. A genetic analysis further reveals that this MAPK cascade is activated by the PYR/PYL/RCAR-SnRK2-PP2C ABA core signalling module through protein synthesis of the MAP3Ks, unveiling an atypical mechanism for MAPK activation in eukaryotes. Our work provides evidence for a role of an ABA-induced MAPK pathway in plant stress signalling. Significance Statement We report in this article the identification of a complete MAPK module, composed of MAP3K17/18, MKK3 and MPK1/2/7/14, which is activated by ABA through the ABA core signalling complex. We showed that the activation of this module requires the MAP3K protein synthesis which occurs after hours of stress treatment, suggesting that the pathway is involved in a delayed wave of cellular responses to ABA and drought. © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.en
dc.description.sponsorshipWe thank Sean Cutler, Sylvain Merlot and Jane Parker for kindly providing hab1G246D line, pyr1pyl1pyl2pyl4 mutant and pEXCS-GW-mYFP-nls plasmid, respectively. This work was supported by the Institut National de Recherche Agronomique and the Agence Nationale de la Recherche. AD was supported by the Ghanaian Ministry of Education.en
dc.publisherWiley-Blackwellen
dc.subjectabscisic aciden
dc.subjectArabidopsis thalianaen
dc.subjectMAP3K18en
dc.subjectMAPK moduleen
dc.subjectMKK3en
dc.subjectsignalling pathwayen
dc.titleIdentification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thalianaen
dc.typeArticleen
dc.contributor.departmentCenter for Desert Agricultureen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.identifier.journalThe Plant Journalen
dc.contributor.institutionUniv Evry Val dEssonne, Inst Plant Sci Paris Saclay, Inst Natl Rech Agron, CNRS,Univ Paris Sud,Univ Paris Diderot,Saclay Pla, F-91405 Orsay, Franceen
dc.contributor.institutionUniv Aix Marseille 2, Inst Biol Environm & Biotechnol, CNRS, Commissariat Energie Atom, F-13108 St Paul Les Durance, Franceen
dc.contributor.institutionInst Pasteur, F-75015 Paris, Franceen
dc.contributor.institutionUniv Valencia, Biochem & Mol Biol Dept, Fac Farm, E-46100 Burjassot, Spainen
kaust.authorHirt, Heriberten
kaust.authorZélicourt, Axel deen
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