Allochthonous bioaugmentation in ex situ treatment of crude oil-polluted sediments in the presence of an effective degrading indigenous microbiome

Handle URI:
http://hdl.handle.net/10754/564116
Title:
Allochthonous bioaugmentation in ex situ treatment of crude oil-polluted sediments in the presence of an effective degrading indigenous microbiome
Authors:
Fodelianakis, Stylianos ( 0000-0003-2186-6009 ) ; Antoniou, E. A.; Mapelli, Francesca; Magagnini, Mirko; Nikolopoulou, Maria; Marasco, Ramona ( 0000-0003-4776-7519 ) ; Barbato, Marta; Tsiola, Areti; Tsikopoulou, I.; Giaccaglia, L.; Mahjoubi, Mouna; Jaouani, Atef; Amer, R.; Hussein, Emad I.; Al-Horani, Fuad A.; Benzha, Fatiha; Blaghen, Mohamed; Malkawi, Hanan Issa; Abdel-Fattah, Yasser Refaat; Cherif, Ameur; Daffonchio, Daniele ( 0000-0003-0947-925X ) ; Kalogerakis, Nicolas E.
Abstract:
Oil-polluted sediment bioremediation depends on both physicochemical and biological parameters, but the effect of the latter cannot be evaluated without the optimization of the former. We aimed in optimizing the physicochemical parameters related to biodegradation by applying an ex-situ landfarming set-up combined with biostimulation to oil-polluted sediment, in order to determine the added effect of bioaugmentation by four allochthonous oil-degrading bacterial consortia in relation to the degradation efficiency of the indigenous community. We monitored hydrocarbon degradation, sediment ecotoxicity and hydrolytic activity, bacterial population sizes and bacterial community dynamics, characterizing the dominant taxa through time and at each treatment. We observed no significant differences in total degradation, but increased ecotoxicity between the different treatments receiving both biostimulation and bioaugmentation and the biostimulated-only control. Moreover, the added allochthonous bacteria quickly perished and were rarely detected, their addition inducing minimal shifts in community structure although it altered the distribution of the residual hydrocarbons in two treatments. Therefore, we concluded that biodegradation was mostly performed by the autochthonous populations while bioaugmentation, in contrast to biostimulation, did not enhance the remediation process. Our results indicate that when environmental conditions are optimized, the indigenous microbiome at a polluted site will likely outperform any allochthonous consortium.
KAUST Department:
Biological and Environmental Sciences and Engineering (BESE) Division; Bioscience Program; Extreme Systems Microbiology Lab
Publisher:
Elsevier BV
Journal:
Journal of Hazardous Materials
Issue Date:
Apr-2015
DOI:
10.1016/j.jhazmat.2015.01.038
Type:
Article
ISSN:
03043894
Sponsors:
This work was funded by FP-7 PROJECT No. 266473, "Unravelling and exploiting Mediterranean Sea microbial diversity and ecology for xenobiotics' and pollutants' clean up" - ULIXES. The authors would like to thank Prof. Nico Boon for his valuable advice and the five anonymous reviewers for their constructive comments. Francesca Mapelli was supported by Universita degli Studi di Milano, DeFENS, European Social Found (FSE) and Regione Lombardia (contract "Dote Ricerca").
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorFodelianakis, Stylianosen
dc.contributor.authorAntoniou, E. A.en
dc.contributor.authorMapelli, Francescaen
dc.contributor.authorMagagnini, Mirkoen
dc.contributor.authorNikolopoulou, Mariaen
dc.contributor.authorMarasco, Ramonaen
dc.contributor.authorBarbato, Martaen
dc.contributor.authorTsiola, Aretien
dc.contributor.authorTsikopoulou, I.en
dc.contributor.authorGiaccaglia, L.en
dc.contributor.authorMahjoubi, Mounaen
dc.contributor.authorJaouani, Atefen
dc.contributor.authorAmer, R.en
dc.contributor.authorHussein, Emad I.en
dc.contributor.authorAl-Horani, Fuad A.en
dc.contributor.authorBenzha, Fatihaen
dc.contributor.authorBlaghen, Mohameden
dc.contributor.authorMalkawi, Hanan Issaen
dc.contributor.authorAbdel-Fattah, Yasser Refaaten
dc.contributor.authorCherif, Ameuren
dc.contributor.authorDaffonchio, Danieleen
dc.contributor.authorKalogerakis, Nicolas E.en
dc.date.accessioned2015-08-03T12:32:51Zen
dc.date.available2015-08-03T12:32:51Zen
dc.date.issued2015-04en
dc.identifier.issn03043894en
dc.identifier.doi10.1016/j.jhazmat.2015.01.038en
dc.identifier.urihttp://hdl.handle.net/10754/564116en
dc.description.abstractOil-polluted sediment bioremediation depends on both physicochemical and biological parameters, but the effect of the latter cannot be evaluated without the optimization of the former. We aimed in optimizing the physicochemical parameters related to biodegradation by applying an ex-situ landfarming set-up combined with biostimulation to oil-polluted sediment, in order to determine the added effect of bioaugmentation by four allochthonous oil-degrading bacterial consortia in relation to the degradation efficiency of the indigenous community. We monitored hydrocarbon degradation, sediment ecotoxicity and hydrolytic activity, bacterial population sizes and bacterial community dynamics, characterizing the dominant taxa through time and at each treatment. We observed no significant differences in total degradation, but increased ecotoxicity between the different treatments receiving both biostimulation and bioaugmentation and the biostimulated-only control. Moreover, the added allochthonous bacteria quickly perished and were rarely detected, their addition inducing minimal shifts in community structure although it altered the distribution of the residual hydrocarbons in two treatments. Therefore, we concluded that biodegradation was mostly performed by the autochthonous populations while bioaugmentation, in contrast to biostimulation, did not enhance the remediation process. Our results indicate that when environmental conditions are optimized, the indigenous microbiome at a polluted site will likely outperform any allochthonous consortium.en
dc.description.sponsorshipThis work was funded by FP-7 PROJECT No. 266473, "Unravelling and exploiting Mediterranean Sea microbial diversity and ecology for xenobiotics' and pollutants' clean up" - ULIXES. The authors would like to thank Prof. Nico Boon for his valuable advice and the five anonymous reviewers for their constructive comments. Francesca Mapelli was supported by Universita degli Studi di Milano, DeFENS, European Social Found (FSE) and Regione Lombardia (contract "Dote Ricerca").en
dc.publisherElsevier BVen
dc.subjectAllochthonous degradersen
dc.subjectAutochthonous degradersen
dc.subjectBioaugmentationen
dc.subjectBioremediationen
dc.subjectBiostimulationen
dc.subjectLandfarmingen
dc.subjectPetroleumen
dc.titleAllochthonous bioaugmentation in ex situ treatment of crude oil-polluted sediments in the presence of an effective degrading indigenous microbiomeen
dc.typeArticleen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentExtreme Systems Microbiology Laben
dc.identifier.journalJournal of Hazardous Materialsen
dc.eprint.versionPost-printen
dc.contributor.institutionSchool of Environmental Engineering, Technical University of CreteChania, Greeceen
dc.contributor.institutionDepartment of Food, Environment and Nutritional Sciences, University of Milan, Italyen
dc.contributor.institutionEcoTechSystems S.r.l., Italyen
dc.contributor.institutionHellenic Center for Marine ResearchHeraklion Crete, Greeceen
dc.contributor.institutionDepartment of Biology, University of CreteHeraklion Crete, Greeceen
dc.contributor.institutionLR11-ES31 Biotechnology and Bio-Geo Resources Valorization, Higher Institute for Biotechnology, Biotechpole SidiThabet University of ManoubaAriana, Tunisiaen
dc.contributor.institutionLaboratory of Microorganisms and Active Biomolecules, Faculty of Sciences of Tunis, University of Tunis El ManarTunis, Tunisiaen
dc.contributor.institutionGenetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications (SRTA-City)Alexandria, Egypten
dc.contributor.institutionDepartment of Biological Sciences, Yarmouk UniversityIrbid, Jordanen
dc.contributor.institutionFaculty of Marine Sciences, The University of Jordan-AqabaAqaba, Jordanen
dc.contributor.institutionLaboratory of Microbiolgy Biotechnology and Environmrent, University Hassan II Casablanca, Faculty of Sciences aîn-chock, B.P. 5366, Moroccoen
dc.contributor.institutionHamdan Bin Mohammed Smart UniversityAcademic City Dubai, United Arab Emiratesen
kaust.authorFodelianakis, Stylianosen
kaust.authorMarasco, Ramonaen
kaust.authorDaffonchio, Danieleen
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