Hydrophilic interaction liquid chromatography method for measuring the composition of aquatic humic substances

Handle URI:
http://hdl.handle.net/10754/563993
Title:
Hydrophilic interaction liquid chromatography method for measuring the composition of aquatic humic substances
Authors:
Wang, Renqi; Gutiérrez, Leonardo A.; Ng, Siuchoon; Croue, Jean-Philippe
Abstract:
A hydrophilic interaction liquid chromatography (HILIC) method was developed to measure the composition of humic substances from river, reservoir, and treated wastewater based on their physicochemical properties. The current method fractionates the humic substances into four well-defined groups based on parallel analyses with a neutral and a cationic HILIC column, using mobile phases of varied compositions and pH. The results indicate that: (i) the proportion of carboxylic acids in the humic substances from terrestrial origins is less than half of that from treated wastewater (Jeddah, KSA), (ii) a higher content of basic compounds was observed in the humic substances from treated wastewater and Ribou Reservoir (Cholet, France) than in the sample from Loire River (France), (iii) a higher percentage of hydrophobic macromolecules were found in the humic substances from Loire River than in the other samples, and (iv) humic substances of treated wastewater contained less ionic neutral compounds (i.e., pKa 5-9) than the waters from terrestrial origins. The physicochemical property disparity amongst the compounds in each humic substances sample was also evaluated. The humic substances from the lightly humic Loire river displayed the highest disparity, whereas the highly humic Suwannee river (Georgia, USA) showed the most homogeneous humic substances.
KAUST Department:
Water Desalination and Reuse Research Center (WDRC); Water Desalination & Reuse Research Cntr; Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Elsevier BV
Journal:
Analytica Chimica Acta
Issue Date:
Jan-2015
DOI:
10.1016/j.aca.2014.09.026
Type:
Article
ISSN:
00032670
Appears in Collections:
Articles; Water Desalination and Reuse Research Center (WDRC); Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Renqien
dc.contributor.authorGutiérrez, Leonardo A.en
dc.contributor.authorNg, Siuchoonen
dc.contributor.authorCroue, Jean-Philippeen
dc.date.accessioned2015-08-03T12:22:16Zen
dc.date.available2015-08-03T12:22:16Zen
dc.date.issued2015-01en
dc.identifier.issn00032670en
dc.identifier.doi10.1016/j.aca.2014.09.026en
dc.identifier.urihttp://hdl.handle.net/10754/563993en
dc.description.abstractA hydrophilic interaction liquid chromatography (HILIC) method was developed to measure the composition of humic substances from river, reservoir, and treated wastewater based on their physicochemical properties. The current method fractionates the humic substances into four well-defined groups based on parallel analyses with a neutral and a cationic HILIC column, using mobile phases of varied compositions and pH. The results indicate that: (i) the proportion of carboxylic acids in the humic substances from terrestrial origins is less than half of that from treated wastewater (Jeddah, KSA), (ii) a higher content of basic compounds was observed in the humic substances from treated wastewater and Ribou Reservoir (Cholet, France) than in the sample from Loire River (France), (iii) a higher percentage of hydrophobic macromolecules were found in the humic substances from Loire River than in the other samples, and (iv) humic substances of treated wastewater contained less ionic neutral compounds (i.e., pKa 5-9) than the waters from terrestrial origins. The physicochemical property disparity amongst the compounds in each humic substances sample was also evaluated. The humic substances from the lightly humic Loire river displayed the highest disparity, whereas the highly humic Suwannee river (Georgia, USA) showed the most homogeneous humic substances.en
dc.publisherElsevier BVen
dc.subjectElectrostatic forceen
dc.subjectHILICen
dc.subjectHumic substanceen
dc.subjectHydrophilic interactionen
dc.subjectNatural organic matteren
dc.subjectPhysicochemical propertyen
dc.titleHydrophilic interaction liquid chromatography method for measuring the composition of aquatic humic substancesen
dc.typeArticleen
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)en
dc.contributor.departmentWater Desalination & Reuse Research Cntren
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalAnalytica Chimica Actaen
dc.contributor.institutionCollege of Chemistry and Chemical Engineering, Lanzhou UniversityLanzhou, Chinaen
dc.contributor.institutionFacultad del Mary Medio Ambiente, Universidad del PacificoGuayaquil, Ecuadoren
dc.contributor.institutionSchool of Chemical and Biomolecular Engineering, Nanyang Technological University62 Nanyang Drive, Singaporeen
kaust.authorWang, Renqien
kaust.authorCroue, Jean-Philippeen
kaust.authorGutiérrez, Leonardo A.en
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