Proteomic changes in brain tissues of marine medaka (Oryzias melastigma) after chronic exposure to two antifouling compounds: Butenolide and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT)

Handle URI:
http://hdl.handle.net/10754/563888
Title:
Proteomic changes in brain tissues of marine medaka (Oryzias melastigma) after chronic exposure to two antifouling compounds: Butenolide and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT)
Authors:
Chen, Lianguo; Zhang, Huoming ( 0000-0001-5416-0358 ) ; Sun, Jin; Wong, Yuehim; Han, Zhuang; Au, Doris W T; Bajic, Vladimir B. ( 0000-0001-5435-4750 ) ; Qian, Peiyuan
Abstract:
SeaNine 211 with active ingredient of 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) has been used as a "green" antifouling agent worldwide but has raised serious biosafety concerns in coastal environments. DCOIT has the potential to disrupt the neurotransmission in nervous system, but the underlying mechanism has not been clarified. In the present study, we used TMT six-plex labeling coupled with two-dimensional LC-MS/MS analysis to investigate the protein expression profiles in brain tissues of the marine medaka (Oryzias melastigma) after a 28-day exposure to environmentally-realistic concentration of DCOIT at 2.55. μg/L (0.009. μM) or butenolide, one promising antifouling compound, at 2.31. μg/L (0.012. μM). DCOIT and butenolide induced differential expression of 26 and 18 proteins in male brains and of 27 and 23 proteins in female brains, respectively. Distinct mechanisms of toxicity were initiated by DCOIT and butenolide in males, whereas the protein expression profiles were largely similar in females treated by these two compounds. In males, DCOIT exposure mainly led to disruption of mitogen-activated protein kinase (MAPK) signaling pathway, while butenolide affected proteins related to the cytoskeletal disorganization that is considered as a general response to toxicant stress. Furthermore, a sex-dependent protein expression profile was also noted between male and female fish, as evident by the inverse changes in the expressions of common proteins (5 proteins for butenolide- and 2 proteins for DCOIT-exposed fish). Overall, this study provided insight into the molecular mechanisms underlying the toxicity of DCOIT and butenolide. The extremely low concentrations used in this study highlighted the ecological relevance, arguing for thorough assessments of their ecological risks before the commercialization of any new antifouling compound.
KAUST Department:
Biosciences Core Lab; Computational Bioscience Research Center (CBRC); Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division; Applied Mathematics and Computational Science Program
Publisher:
Elsevier BV
Journal:
Aquatic Toxicology
Issue Date:
Dec-2014
DOI:
10.1016/j.aquatox.2014.09.010
Type:
Article
ISSN:
0166445X
Sponsors:
This study was supported by grants from China Mineral Resources Research and Development Association (COMR-RDA12SC01) and from the Research Grant Council of HKSAR government (662413), and the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and the State Key Laboratory in Marine Pollution, City University of Hong Kong. VB was supported by the Base Research Fund from King Abdullah University of Science and Technology.
Appears in Collections:
Articles; Applied Mathematics and Computational Science Program; Computational Bioscience Research Center (CBRC); Bioscience Core Lab; Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Lianguoen
dc.contributor.authorZhang, Huomingen
dc.contributor.authorSun, Jinen
dc.contributor.authorWong, Yuehimen
dc.contributor.authorHan, Zhuangen
dc.contributor.authorAu, Doris W Ten
dc.contributor.authorBajic, Vladimir B.en
dc.contributor.authorQian, Peiyuanen
dc.date.accessioned2015-08-03T12:18:26Zen
dc.date.available2015-08-03T12:18:26Zen
dc.date.issued2014-12en
dc.identifier.issn0166445Xen
dc.identifier.doi10.1016/j.aquatox.2014.09.010en
dc.identifier.urihttp://hdl.handle.net/10754/563888en
dc.description.abstractSeaNine 211 with active ingredient of 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT) has been used as a "green" antifouling agent worldwide but has raised serious biosafety concerns in coastal environments. DCOIT has the potential to disrupt the neurotransmission in nervous system, but the underlying mechanism has not been clarified. In the present study, we used TMT six-plex labeling coupled with two-dimensional LC-MS/MS analysis to investigate the protein expression profiles in brain tissues of the marine medaka (Oryzias melastigma) after a 28-day exposure to environmentally-realistic concentration of DCOIT at 2.55. μg/L (0.009. μM) or butenolide, one promising antifouling compound, at 2.31. μg/L (0.012. μM). DCOIT and butenolide induced differential expression of 26 and 18 proteins in male brains and of 27 and 23 proteins in female brains, respectively. Distinct mechanisms of toxicity were initiated by DCOIT and butenolide in males, whereas the protein expression profiles were largely similar in females treated by these two compounds. In males, DCOIT exposure mainly led to disruption of mitogen-activated protein kinase (MAPK) signaling pathway, while butenolide affected proteins related to the cytoskeletal disorganization that is considered as a general response to toxicant stress. Furthermore, a sex-dependent protein expression profile was also noted between male and female fish, as evident by the inverse changes in the expressions of common proteins (5 proteins for butenolide- and 2 proteins for DCOIT-exposed fish). Overall, this study provided insight into the molecular mechanisms underlying the toxicity of DCOIT and butenolide. The extremely low concentrations used in this study highlighted the ecological relevance, arguing for thorough assessments of their ecological risks before the commercialization of any new antifouling compound.en
dc.description.sponsorshipThis study was supported by grants from China Mineral Resources Research and Development Association (COMR-RDA12SC01) and from the Research Grant Council of HKSAR government (662413), and the King Abdullah University of Science and Technology (SA-C0040/UK-C0016) and the State Key Laboratory in Marine Pollution, City University of Hong Kong. VB was supported by the Base Research Fund from King Abdullah University of Science and Technology.en
dc.publisherElsevier BVen
dc.subjectBrain neurotoxicityen
dc.subjectButenolideen
dc.subjectDCOITen
dc.subjectGender differenceen
dc.subjectMarine medakaen
dc.subjectProteomeen
dc.titleProteomic changes in brain tissues of marine medaka (Oryzias melastigma) after chronic exposure to two antifouling compounds: Butenolide and 4,5-dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT)en
dc.typeArticleen
dc.contributor.departmentBiosciences Core Laben
dc.contributor.departmentComputational Bioscience Research Center (CBRC)en
dc.contributor.departmentComputer, Electrical and Mathematical Sciences and Engineering (CEMSE) Divisionen
dc.contributor.departmentApplied Mathematics and Computational Science Programen
dc.identifier.journalAquatic Toxicologyen
dc.contributor.institutionDivision of Life Science and Environmental Science Programs, Hong Kong University of Science and Technology, Hong Kongen
dc.contributor.institutionDepartment of Biology, Hong Kong Baptist University, Hong Kongen
dc.contributor.institutionState Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Hong Kongen
kaust.authorZhang, Huomingen
kaust.authorBajic, Vladimir B.en
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