Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells

Handle URI:
http://hdl.handle.net/10754/563624
Title:
Folic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cells
Authors:
Malara, Natalia; Coluccio, Maria Laura; Limongi, Tania; Asande, Monica; Trunzo, Valentina; Cojoc, Gheorghe; Raso, Cinzia; Candeloro, Patrizio; Perozziello, Gerardo; Raimondo, Raffaella; De Vitis, Stefania; Roveda, Laura; Renne, Maria; Prati, Ubaldo; Mollace, Vincenzo; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
Although the detection of methylated cell free DNA represents one of the most promising approaches for relapse risk assessment in cancer patients, the low concentration of cell-free circulating DNA constitutes the biggest obstacle in the development of DNA methylation-based biomarkers from blood. This paper describes a method for the measurement of genomic methylation content directly on circulating tumor cells (CTC), which could be used to deceive the aforementioned problem. Since CTC are disease related blood-based biomarkers, they result essential to monitor tumor's stadiation, therapy, and early relapsing lesions. Within surface's bio-functionalization and cell's isolation procedure standardization, the presented approach reveals a singular ability to detect high 5-methylcytosine CTC-subset content in the whole CTC compound, by choosing folic acid (FA) as transducer molecule. Sensitivity and specificity, calculated for FA functionalized surface (FA-surface), result respectively on about 83% and 60%. FA-surface, allowing the detection and characterization of early metastatic dissemination, provides a unique advance in the comprehension of tumors progression and dissemination confirming the presence of CTC and its association with high risk of relapse. This functionalized surface identifying and quantifying high 5-methylcytosine CTC-subset content into the patient's blood lead significant progress in cancer risk assessment, also providing a novel therapeutic strategy.© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KAUST Department:
Physical Sciences and Engineering (PSE) Division
Publisher:
Wiley-VCH Verlag
Journal:
Small
Issue Date:
Jul-2014
DOI:
10.1002/smll.201400498; 10.1002/smll.201470135
Type:
Article
ISSN:
16136810
Sponsors:
This work was partially supported by the Grants from the PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" project (code: PON01_02782), the Interregional Research Centre for Food Safety & Health (IRC_FSC) project (cod. PON a3-00359) granted to the Department of Health Science of the University Magna Graecia of Catanzaro, the FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" project (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro and the "Fondo Sociale Europeo - POR Calabria FSE 2007/2013" Program. The authors thank R. Giammaria for revising the English text.
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorMalara, Nataliaen
dc.contributor.authorColuccio, Maria Lauraen
dc.contributor.authorLimongi, Taniaen
dc.contributor.authorAsande, Monicaen
dc.contributor.authorTrunzo, Valentinaen
dc.contributor.authorCojoc, Gheorgheen
dc.contributor.authorRaso, Cinziaen
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorPerozziello, Gerardoen
dc.contributor.authorRaimondo, Raffaellaen
dc.contributor.authorDe Vitis, Stefaniaen
dc.contributor.authorRoveda, Lauraen
dc.contributor.authorRenne, Mariaen
dc.contributor.authorPrati, Ubaldoen
dc.contributor.authorMollace, Vincenzoen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-08-03T12:04:42Zen
dc.date.available2015-08-03T12:04:42Zen
dc.date.issued2014-07en
dc.identifier.issn16136810en
dc.identifier.doi10.1002/smll.201400498en
dc.identifier.doi10.1002/smll.201470135en
dc.identifier.urihttp://hdl.handle.net/10754/563624en
dc.description.abstractAlthough the detection of methylated cell free DNA represents one of the most promising approaches for relapse risk assessment in cancer patients, the low concentration of cell-free circulating DNA constitutes the biggest obstacle in the development of DNA methylation-based biomarkers from blood. This paper describes a method for the measurement of genomic methylation content directly on circulating tumor cells (CTC), which could be used to deceive the aforementioned problem. Since CTC are disease related blood-based biomarkers, they result essential to monitor tumor's stadiation, therapy, and early relapsing lesions. Within surface's bio-functionalization and cell's isolation procedure standardization, the presented approach reveals a singular ability to detect high 5-methylcytosine CTC-subset content in the whole CTC compound, by choosing folic acid (FA) as transducer molecule. Sensitivity and specificity, calculated for FA functionalized surface (FA-surface), result respectively on about 83% and 60%. FA-surface, allowing the detection and characterization of early metastatic dissemination, provides a unique advance in the comprehension of tumors progression and dissemination confirming the presence of CTC and its association with high risk of relapse. This functionalized surface identifying and quantifying high 5-methylcytosine CTC-subset content into the patient's blood lead significant progress in cancer risk assessment, also providing a novel therapeutic strategy.© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.en
dc.description.sponsorshipThis work was partially supported by the Grants from the PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" project (code: PON01_02782), the Interregional Research Centre for Food Safety & Health (IRC_FSC) project (cod. PON a3-00359) granted to the Department of Health Science of the University Magna Graecia of Catanzaro, the FIRB "Rete Nazionale di Ricerca sulle Nanoscienze ItalNanoNet" project (cod. RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro and the "Fondo Sociale Europeo - POR Calabria FSE 2007/2013" Program. The authors thank R. Giammaria for revising the English text.en
dc.publisherWiley-VCH Verlagen
dc.subjectFolic aciden
dc.subjectFolic acid receptorsen
dc.subjectFunctionalized surfacesen
dc.subjectHyper-methylated circulating tumor cellsen
dc.titleFolic acid functionalized surface highlights 5-methylcytosine-genomic content within circulating tumor cellsen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.identifier.journalSmallen
dc.contributor.institutionItalian Institute of TechnologyArnesano, Lecce, Italyen
dc.contributor.institutionBIONEM, University of Magna Graecia, Campus S. Venuta, Viale EuropaCatanzaro, Italyen
dc.contributor.institutionFaculty of Pharmacy, University of Magna Graecia, Campus Salvatore Venuta, Viale EuropaCatanzaro, Italyen
dc.contributor.institutionMax Planck Institute of Molecular, Cell Biology and Genetics, Pfotenhauerstrasse 108Dresden, Germanyen
dc.contributor.institutionSystems Biology Ireland, Conway Institute UCDDublin, Belfield, Irelanden
dc.contributor.institutionOncologic Surgery, Cancer Centre of Excellence Tommaso Campanella Foundation, Viale EuropaCatanzaro, Italyen
dc.contributor.institutionInterregional Research Center on Food Safety and Health (IRC-FSH), Department of Health Sciences, University Magna Graecia of CatanzaroCatanzaro, Italyen
kaust.authorLimongi, Taniaen
kaust.authorDi Fabrizio, Enzo M.en
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