Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols

Handle URI:
http://hdl.handle.net/10754/563614
Title:
Isolation of cancer cells by "in situ" microfluidic biofunctionalization protocols
Authors:
De Vitis, Stefania; Matarise, Giuseppina; Pardeo, Francesca; Catalano, Rossella; Malara, Natalia Maria; Trunzo, Valentina; Tallerico, Rossana; Gentile, Francesco T.; Candeloro, Patrizio; Coluccio, Maria Laura; Massaro, Alessandro S.; Viglietto, Giuseppe; Carbone, Ennio; Kutter, Jörg Peter; Perozziello, Gerardo; Di Fabrizio, Enzo M. ( 0000-0001-5886-4678 )
Abstract:
The aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs. © 2014 Elsevier B.V. All rights reserved.
KAUST Department:
Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program
Publisher:
Elsevier BV
Journal:
Microelectronic Engineering
Issue Date:
Jul-2014
DOI:
10.1016/j.mee.2014.04.013
Type:
Article
ISSN:
01679317
Sponsors:
This work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (code: PON01_02782), the European project EUROMBR (grant agreement n.608104), the project for Young Researchers financed from the Italian Ministry of Health (CUP J65C13001350001, project n. GR-2010-2311677) and the project FIRB "ReteNazionale di Ricerca sulle Nanoscienze ItalNanoNet" (code: RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro.
Appears in Collections:
Articles; Physical Sciences and Engineering (PSE) Division; Materials Science and Engineering Program

Full metadata record

DC FieldValue Language
dc.contributor.authorDe Vitis, Stefaniaen
dc.contributor.authorMatarise, Giuseppinaen
dc.contributor.authorPardeo, Francescaen
dc.contributor.authorCatalano, Rossellaen
dc.contributor.authorMalara, Natalia Mariaen
dc.contributor.authorTrunzo, Valentinaen
dc.contributor.authorTallerico, Rossanaen
dc.contributor.authorGentile, Francesco T.en
dc.contributor.authorCandeloro, Patrizioen
dc.contributor.authorColuccio, Maria Lauraen
dc.contributor.authorMassaro, Alessandro S.en
dc.contributor.authorViglietto, Giuseppeen
dc.contributor.authorCarbone, Ennioen
dc.contributor.authorKutter, Jörg Peteren
dc.contributor.authorPerozziello, Gerardoen
dc.contributor.authorDi Fabrizio, Enzo M.en
dc.date.accessioned2015-08-03T12:04:21Zen
dc.date.available2015-08-03T12:04:21Zen
dc.date.issued2014-07en
dc.identifier.issn01679317en
dc.identifier.doi10.1016/j.mee.2014.04.013en
dc.identifier.urihttp://hdl.handle.net/10754/563614en
dc.description.abstractThe aim of this work is the development of a microfluidic immunosensor for the immobilization of cancer cells and their separation from healthy cells by using "in situ" microfluidic biofunctionalization protocols. These protocols allow to link antibodies on microfluidic device surfaces and can be used to study the interaction between cell membrane and biomolecules. Moreover they allow to perform analysis with high processing speed, small quantity of reagents and samples, short reaction times and low production costs. In this work the developed protocols were used in microfluidic devices for the isolation of cancer cells in heterogeneous blood samples by exploiting the binding of specific antibody to an adhesion protein (EpCAM), overexpressed on the tumor cell membranes. The presented biofunctionalization protocols can be performed right before running the experiment: this allows to have a flexible platform where biomolecules of interest can be linked on the device surface according to the user's needs. © 2014 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipThis work was partially supported by the project PON "Nuove strategie nanotecnologiche per la messa a punto di farmaci e presidi diagnostici diretti verso cellule cancerose circolanti" (code: PON01_02782), the European project EUROMBR (grant agreement n.608104), the project for Young Researchers financed from the Italian Ministry of Health (CUP J65C13001350001, project n. GR-2010-2311677) and the project FIRB "ReteNazionale di Ricerca sulle Nanoscienze ItalNanoNet" (code: RBPR05JH2P_010, CUP B41J09000110005) granted to the nanotechnology laboratory of the Department of Experimental Medicine of the University of Magna Graecia of Catanzaro.en
dc.publisherElsevier BVen
dc.subjectBiofunctionalization protocolsen
dc.subjectCancer cell isolationsen
dc.subjectCell sortingen
dc.subjectMicrofluidic assayen
dc.titleIsolation of cancer cells by "in situ" microfluidic biofunctionalization protocolsen
dc.typeArticleen
dc.contributor.departmentPhysical Sciences and Engineering (PSE) Divisionen
dc.contributor.departmentMaterials Science and Engineering Programen
dc.identifier.journalMicroelectronic Engineeringen
dc.contributor.institutionItalian Institute of Technology IIT, Genova, Italyen
dc.contributor.institutionUniversity of Catanzaro magna Græcia, 88100, Catanzaro, Italyen
dc.contributor.institutionDepartment of Pharmacy, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmarken
dc.contributor.institutionDepartment of Microbiology Tumor and Cell Biology (MTC), Karolinska Institutet, 17177, Stockolm, Swedenen
dc.contributor.institutionIstituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies (CBN), Via Barsanti, 73010 Arnesano (LE), Italyen
kaust.authorDi Fabrizio, Enzo M.en
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