Coral transcriptome and bacterial community profiles reveal distinct Yellow Band Disease states in Orbicella faveolata

Handle URI:
http://hdl.handle.net/10754/563606
Title:
Coral transcriptome and bacterial community profiles reveal distinct Yellow Band Disease states in Orbicella faveolata
Authors:
Closek, Collin J.; Sunagawa, Shinichi; DeSalvo, Michael K.; Piceno, Yvette M.; Desantis, Todd Z.; Brodie, Eoin L.; Weber, Michele X.; Voolstra, Christian R. ( 0000-0003-4555-3795 ) ; Andersen, Gary L.; Medina, Mónica M.
Abstract:
Coral diseases impact reefs globally. Although we continue to describe diseases, little is known about the etiology or progression of even the most common cases. To examine a spectrum of coral health and determine factors of disease progression we examined Orbicella faveolata exhibiting signs of Yellow Band Disease (YBD), a widespread condition in the Caribbean. We used a novel combined approach to assess three members of the coral holobiont: the coral-host, associated Symbiodinium algae, and bacteria. We profiled three conditions: (1) healthy-appearing colonies (HH), (2) healthy-appearing tissue on diseased colonies (HD), and (3) diseased lesion (DD). Restriction fragment length polymorphism analysis revealed health state-specific diversity in Symbiodinium clade associations. 16S ribosomal RNA gene microarrays (PhyloChips) and O. faveolata complimentary DNA microarrays revealed the bacterial community structure and host transcriptional response, respectively. A distinct bacterial community structure marked each health state. Diseased samples were associated with two to three times more bacterial diversity. HD samples had the highest bacterial richness, which included components associated with HH and DD, as well as additional unique families. The host transcriptome under YBD revealed a reduced cellular expression of defense- and metabolism-related processes, while the neighboring HD condition exhibited an intermediate expression profile. Although HD tissue appeared visibly healthy, the microbial communities and gene expression profiles were distinct. HD should be regarded as an additional (intermediate) state of disease, which is important for understanding the progression of YBD. © 2014 International Society for Microbial Ecology. All rights reserved.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Reef Genomics Lab
Publisher:
Springer Nature
Journal:
The ISME Journal
Issue Date:
20-Jun-2014
DOI:
10.1038/ismej.2014.85
PubMed ID:
24950107
PubMed Central ID:
PMC4260706
Type:
Article
ISSN:
17517362
Sponsors:
We would like to thank the Instituto de Ciencias del Mar y Limnologia (ICML), the Universidad Nacional Autonoma de Mexico for providing facilities and collection permits. Additionally we would like to thank those at the ICML, as well as the Medina & Andersen Lab members who provided assistance in collecting the sample, and in experimental and analytical methods. Especially, Adan Guillermo Jordan-Garza, Julia Schnetzer and Erika M Diaz-Almeyda for helping with the sample collection. Nicholas R Polato & Elizabeth Green for genotyping coral colonies. Lauren M Tom for assistance with the analyses. Justin L Matthews for statistical input. Bishoy SK Kamel and Erika M Diaz-Almeyda for additional draft comments. This study was supported by NSF awards IOS 0644438 and IOS 0926906 from NSF to MM.
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4260706
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Marine Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorClosek, Collin J.en
dc.contributor.authorSunagawa, Shinichien
dc.contributor.authorDeSalvo, Michael K.en
dc.contributor.authorPiceno, Yvette M.en
dc.contributor.authorDesantis, Todd Z.en
dc.contributor.authorBrodie, Eoin L.en
dc.contributor.authorWeber, Michele X.en
dc.contributor.authorVoolstra, Christian R.en
dc.contributor.authorAndersen, Gary L.en
dc.contributor.authorMedina, Mónica M.en
dc.date.accessioned2015-08-03T11:55:32Zen
dc.date.available2015-08-03T11:55:32Zen
dc.date.issued2014-06-20en
dc.identifier.issn17517362en
dc.identifier.pmid24950107en
dc.identifier.doi10.1038/ismej.2014.85en
dc.identifier.urihttp://hdl.handle.net/10754/563606en
dc.description.abstractCoral diseases impact reefs globally. Although we continue to describe diseases, little is known about the etiology or progression of even the most common cases. To examine a spectrum of coral health and determine factors of disease progression we examined Orbicella faveolata exhibiting signs of Yellow Band Disease (YBD), a widespread condition in the Caribbean. We used a novel combined approach to assess three members of the coral holobiont: the coral-host, associated Symbiodinium algae, and bacteria. We profiled three conditions: (1) healthy-appearing colonies (HH), (2) healthy-appearing tissue on diseased colonies (HD), and (3) diseased lesion (DD). Restriction fragment length polymorphism analysis revealed health state-specific diversity in Symbiodinium clade associations. 16S ribosomal RNA gene microarrays (PhyloChips) and O. faveolata complimentary DNA microarrays revealed the bacterial community structure and host transcriptional response, respectively. A distinct bacterial community structure marked each health state. Diseased samples were associated with two to three times more bacterial diversity. HD samples had the highest bacterial richness, which included components associated with HH and DD, as well as additional unique families. The host transcriptome under YBD revealed a reduced cellular expression of defense- and metabolism-related processes, while the neighboring HD condition exhibited an intermediate expression profile. Although HD tissue appeared visibly healthy, the microbial communities and gene expression profiles were distinct. HD should be regarded as an additional (intermediate) state of disease, which is important for understanding the progression of YBD. © 2014 International Society for Microbial Ecology. All rights reserved.en
dc.description.sponsorshipWe would like to thank the Instituto de Ciencias del Mar y Limnologia (ICML), the Universidad Nacional Autonoma de Mexico for providing facilities and collection permits. Additionally we would like to thank those at the ICML, as well as the Medina & Andersen Lab members who provided assistance in collecting the sample, and in experimental and analytical methods. Especially, Adan Guillermo Jordan-Garza, Julia Schnetzer and Erika M Diaz-Almeyda for helping with the sample collection. Nicholas R Polato & Elizabeth Green for genotyping coral colonies. Lauren M Tom for assistance with the analyses. Justin L Matthews for statistical input. Bishoy SK Kamel and Erika M Diaz-Almeyda for additional draft comments. This study was supported by NSF awards IOS 0644438 and IOS 0926906 from NSF to MM.en
dc.publisherSpringer Natureen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4260706en
dc.subject16S rRNA geneen
dc.subjectCoral reefsen
dc.subjectMontastraea faveolataen
dc.subjectOrbicella faveolataen
dc.subjectPhyloChipen
dc.subjectYellow band blotch diseaseen
dc.titleCoral transcriptome and bacterial community profiles reveal distinct Yellow Band Disease states in Orbicella faveolataen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
dc.contributor.departmentReef Genomics Laben
dc.identifier.journalThe ISME Journalen
dc.identifier.pmcidPMC4260706en
dc.contributor.institutionDepartment of Biology, Pennsylvania State University, 208 Mueller Lab BuildingUniversity Park, PA, United Statesen
dc.contributor.institutionSchool of Natural Sciences, University of CaliforniaMerced, CA, United Statesen
dc.contributor.institutionStructural and Computational Biology Unit, European Molecular Biology LaboratoryHeidelberg, Germanyen
dc.contributor.institutionPhalanx Biotech Group Inc.San Diego, CA, United Statesen
dc.contributor.institutionCenter for Environmental Biotechnology, Lawrence Berkeley National LaboratoryBerkeley, CA, United Statesen
dc.contributor.institutionSecond Genome Inc.South San Francisco, CA, United Statesen
kaust.authorVoolstra, Christian R.en

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