Distribution and diversity of Prochlorococcus ecotypes in the Red Sea

Handle URI:
http://hdl.handle.net/10754/563605
Title:
Distribution and diversity of Prochlorococcus ecotypes in the Red Sea
Authors:
Shibl, Ahmed A. ( 0000-0002-8147-8406 ) ; Thompson, Luke R.; Ngugi, David ( 0000-0002-0442-4279 ) ; Stingl, Ulrich ( 0000-0002-0684-2597 )
Abstract:
Photosynthetic prokaryotes of the genus Prochlorococcus play a major role in global primary production in the world's oligotrophic oceans. A recent study on pelagic bacterioplankton communities in the northern and central Red Sea indicated that the predominant cyanobacterial 16S rRNA gene sequence types were from Prochlorococcus cells belonging to a high-light-adapted ecotype (HL II). In this study, we analyzed microdiversity of Prochlorococcus sp. at multiple depths within and below the euphotic zone in the northern, central, and southern regions of the Red Sea, as well as in surface waters in the same locations, but in a different season. Prochlorococcus dominated the communities in clone libraries of the amplified 16S-23S rRNA internal transcribed spacer (ITS) region. Almost no differences were found between samples from coastal or open-water sites, but a high diversity of Prochlorococcus ecotypes was detected at 100-meter depth in the water column. In addition, an unusual dominance of HL II-related sequences was observed in deeper waters. Our results indicate that the Red Sea harbors diverse Prochlorococcus lineages, but no novel ecotypes, despite its unusual physicochemical properties. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.
KAUST Department:
Red Sea Research Center (RSRC); Biological and Environmental Sciences and Engineering (BESE) Division; Marine Science Program; Marine Microbial Ecology Research Group
Publisher:
Oxford University Press (OUP)
Journal:
FEMS Microbiology Letters
Issue Date:
19-Jun-2014
DOI:
10.1111/1574-6968.12490
Type:
Article
ISSN:
03781097
Sponsors:
We thank AbdulAziz Al-Suwailem, the scientists, the captain and the crew of the R/V Aegaeo for the KAUST Red Sea Expeditions in 2010 and 2011, and the Coastal and Marine Resources Core Lab (CMRC) and the Biosciences Core Laboratory (BCL) at King Abdullah University for Science and Technology (KAUST) for their logistical and technical support. This study was supported by the SABIC Career Development Chair (US).
Appears in Collections:
Articles; Red Sea Research Center (RSRC); Marine Science Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorShibl, Ahmed A.en
dc.contributor.authorThompson, Luke R.en
dc.contributor.authorNgugi, Daviden
dc.contributor.authorStingl, Ulrichen
dc.date.accessioned2015-08-03T11:55:30Zen
dc.date.available2015-08-03T11:55:30Zen
dc.date.issued2014-06-19en
dc.identifier.issn03781097en
dc.identifier.doi10.1111/1574-6968.12490en
dc.identifier.urihttp://hdl.handle.net/10754/563605en
dc.description.abstractPhotosynthetic prokaryotes of the genus Prochlorococcus play a major role in global primary production in the world's oligotrophic oceans. A recent study on pelagic bacterioplankton communities in the northern and central Red Sea indicated that the predominant cyanobacterial 16S rRNA gene sequence types were from Prochlorococcus cells belonging to a high-light-adapted ecotype (HL II). In this study, we analyzed microdiversity of Prochlorococcus sp. at multiple depths within and below the euphotic zone in the northern, central, and southern regions of the Red Sea, as well as in surface waters in the same locations, but in a different season. Prochlorococcus dominated the communities in clone libraries of the amplified 16S-23S rRNA internal transcribed spacer (ITS) region. Almost no differences were found between samples from coastal or open-water sites, but a high diversity of Prochlorococcus ecotypes was detected at 100-meter depth in the water column. In addition, an unusual dominance of HL II-related sequences was observed in deeper waters. Our results indicate that the Red Sea harbors diverse Prochlorococcus lineages, but no novel ecotypes, despite its unusual physicochemical properties. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.en
dc.description.sponsorshipWe thank AbdulAziz Al-Suwailem, the scientists, the captain and the crew of the R/V Aegaeo for the KAUST Red Sea Expeditions in 2010 and 2011, and the Coastal and Marine Resources Core Lab (CMRC) and the Biosciences Core Laboratory (BCL) at King Abdullah University for Science and Technology (KAUST) for their logistical and technical support. This study was supported by the SABIC Career Development Chair (US).en
dc.publisherOxford University Press (OUP)en
dc.subjectDiversityen
dc.subjectEcotypesen
dc.subjectProchlorococcusen
dc.subjectRed Seaen
dc.subjectrRNAen
dc.subjectrRNA internal transcribed spaceren
dc.titleDistribution and diversity of Prochlorococcus ecotypes in the Red Seaen
dc.typeArticleen
dc.contributor.departmentRed Sea Research Center (RSRC)en
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.contributor.departmentMarine Science Programen
dc.contributor.departmentMarine Microbial Ecology Research Groupen
dc.identifier.journalFEMS Microbiology Lettersen
dc.contributor.institutionBioFrontiers Institute, University of Colorado, Boulder, CO, United Statesen
kaust.authorThompson, Luke R.en
kaust.authorNgugi, Daviden
kaust.authorStingl, Ulrichen
kaust.authorShibl, Ahmed A.en
All Items in KAUST are protected by copyright, with all rights reserved, unless otherwise indicated.