Adolescent Mouse Takes on An Active Transcriptomic Expression During Postnatal Cerebral Development

Handle URI:
http://hdl.handle.net/10754/563581
Title:
Adolescent Mouse Takes on An Active Transcriptomic Expression During Postnatal Cerebral Development
Authors:
Xu, Wei; Xin, Chengqi; Lin, Qiang; Ding, Feng ( 0000-0001-8237-4062 ) ; Gong, Wei; Zhou, Yuanyuan; Yu, Jun; Cui, Peng ( 0000-0003-3076-0070 ) ; Hu, Songnian
Abstract:
Postnatal cerebral development is a complicated biological process precisely controlled by multiple genes. To understand the molecular mechanism of cerebral development, we compared dynamics of mouse cerebrum transcriptome through three developmental stages using high-throughput RNA-seq technique. Three libraries were generated from the mouse cerebrum at infancy, adolescence and adulthood, respectively. Consequently, 44,557,729 (infancy), 59,257,530 (adolescence) and 72,729,636 (adulthood) reads were produced, which were assembled into 15,344, 16,048 and 15,775 genes, respectively. We found that the overall gene expression level increased from infancy to adolescence and decreased later on upon reaching adulthood. The adolescence cerebrum has the most active gene expression, with expression of a large number of regulatory genes up-regulated and some crucial pathways activated. Transcription factor (TF) analysis suggested the similar dynamics as expression profiling, especially those TFs functioning in neurogenesis differentiation, oligodendrocyte lineage determination and circadian rhythm regulation. Moreover, our data revealed a drastic increase in myelin basic protein (MBP)-coding gene expression in adolescence and adulthood, suggesting that the brain myelin may be generated since mouse adolescence. In addition, differential gene expression analysis indicated the activation of rhythmic pathway, suggesting the function of rhythmic movement since adolescence; Furthermore, during infancy and adolescence periods, gene expression related to axon. repulsion and attraction showed the opposite trends, indicating that axon repulsion was activated after birth, while axon attraction might be activated at the embryonic stage and declined during the postnatal development. Our results from the present study may shed light on the molecular mechanism underlying the postnatal development of the mammalian cerebrum. © 2014 .
KAUST Department:
Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division
Publisher:
Elsevier BV
Journal:
Genomics, Proteomics & Bioinformatics
Issue Date:
Jun-2014
DOI:
10.1016/j.gpb.2014.04.004
PubMed ID:
24953867
PubMed Central ID:
PMC4411375
Type:
Article
ISSN:
16720229
Additional Links:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411375
Appears in Collections:
Articles; Bioscience Program; Biological and Environmental Sciences and Engineering (BESE) Division

Full metadata record

DC FieldValue Language
dc.contributor.authorXu, Weien
dc.contributor.authorXin, Chengqien
dc.contributor.authorLin, Qiangen
dc.contributor.authorDing, Fengen
dc.contributor.authorGong, Weien
dc.contributor.authorZhou, Yuanyuanen
dc.contributor.authorYu, Junen
dc.contributor.authorCui, Pengen
dc.contributor.authorHu, Songnianen
dc.date.accessioned2015-08-03T11:54:57Zen
dc.date.available2015-08-03T11:54:57Zen
dc.date.issued2014-06en
dc.identifier.issn16720229en
dc.identifier.pmid24953867en
dc.identifier.doi10.1016/j.gpb.2014.04.004en
dc.identifier.urihttp://hdl.handle.net/10754/563581en
dc.description.abstractPostnatal cerebral development is a complicated biological process precisely controlled by multiple genes. To understand the molecular mechanism of cerebral development, we compared dynamics of mouse cerebrum transcriptome through three developmental stages using high-throughput RNA-seq technique. Three libraries were generated from the mouse cerebrum at infancy, adolescence and adulthood, respectively. Consequently, 44,557,729 (infancy), 59,257,530 (adolescence) and 72,729,636 (adulthood) reads were produced, which were assembled into 15,344, 16,048 and 15,775 genes, respectively. We found that the overall gene expression level increased from infancy to adolescence and decreased later on upon reaching adulthood. The adolescence cerebrum has the most active gene expression, with expression of a large number of regulatory genes up-regulated and some crucial pathways activated. Transcription factor (TF) analysis suggested the similar dynamics as expression profiling, especially those TFs functioning in neurogenesis differentiation, oligodendrocyte lineage determination and circadian rhythm regulation. Moreover, our data revealed a drastic increase in myelin basic protein (MBP)-coding gene expression in adolescence and adulthood, suggesting that the brain myelin may be generated since mouse adolescence. In addition, differential gene expression analysis indicated the activation of rhythmic pathway, suggesting the function of rhythmic movement since adolescence; Furthermore, during infancy and adolescence periods, gene expression related to axon. repulsion and attraction showed the opposite trends, indicating that axon repulsion was activated after birth, while axon attraction might be activated at the embryonic stage and declined during the postnatal development. Our results from the present study may shed light on the molecular mechanism underlying the postnatal development of the mammalian cerebrum. © 2014 .en
dc.publisherElsevier BVen
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4411375en
dc.subjectCerebrumen
dc.subjectGene expressionen
dc.subjectHigh-throughput sequencingen
dc.subjectPostnatal developmenten
dc.subjectTranscriptomeen
dc.titleAdolescent Mouse Takes on An Active Transcriptomic Expression During Postnatal Cerebral Developmenten
dc.typeArticleen
dc.contributor.departmentBioscience Programen
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Divisionen
dc.identifier.journalGenomics, Proteomics & Bioinformaticsen
dc.identifier.pmcidPMC4411375en
dc.contributor.institutionCAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, Chinaen
kaust.authorDing, Fengen
kaust.authorCui, Pengen

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